# Key Points from #hplusroadmap IRC Discussions

• A participant is designing an in vivo human muscle electroporation device targeting 200V at 1 amp with 10-50ms pulse duration, while noting that bacterial electroporation requires higher voltages (kilovolts) due to cell walls and smaller size.

• Oxford Nanopore Technologies announced a USB stick-sized DNA sequencing device priced at $900, capable of sequencing a human genome in 2 hours for approximately $1000, though some participants expressed skepticism about these claims.

• Researchers discussed microfluidics experiments, including one attempting to reproduce maze learning in C. elegans nematodes using the "shrinky-dink" method to create maze channels approximately 1mm wide.

• 3Scan's brain mapping technology was highlighted for its ability to scan a cubic centimeter of tissue (like a mouse brain) at submicron resolution in under 100 hours, contributing to whole brain emulation research.

• The Science for Life Extension Foundation's work was referenced, with links shared to their regenerative medicine roadmaps, aging research plans, and telomere research.

• Participants debated the theoretical possibility of engineering significantly larger polymerases (500x bigger), discussing whether such modifications would be functional or if multi-protein complexes would be more effective.

• The community shared resources from diyhpl.us containing papers on gene therapy and other research, demonstrating the collaborative nature of open-source biohacking knowledge exchange.