# Key Points from #hplusroadmap IRC Discussion

• Jordan Miller presented groundbreaking research on 3D printing with sugar to create vascular networks for tissue engineering, where the printed sugar structures can be lined with endothelial cells to form functional blood vessels, with the sugar formulation hardening shortly after extrusion at room temperature.

• Loop-Mediated Isothermal Amplification (LAMP) was discussed as an innovative DNA amplification technique that works under isothermal conditions and has been successfully used to detect parasite DNA from processed blood samples.

• A DIY biology project called "BlueGene" was announced, aiming to transfer a gene between bacteria that codes for an enzyme breaking down indole to produce indigo, with an estimated cost under $200 per person, representing accessible biohacking.

• Participants debated climate change framing, noting that concerns are primarily about local energy distribution supporting human survival rather than violations of thermodynamic laws, with technical critiques pointing out that tidal friction and radioactivity are dynamic energy sources.

• A paper on hybrid error correction and de novo assembly of single-molecule sequencing reads was shared, indicating ongoing interest in advanced genomic sequencing technologies among channel participants.

• The community demonstrated technical collaboration through discussions of CAD tools for 3D modeling (including a Python-based approach called "esolid.py") and file transfer protocols, showing the practical, hands-on nature of the transhumanist community.