# Key Points from #hplusroadmap IRC Discussion

• Researchers discussed nanopore DNA sequencing technology, specifically referencing a Nature Biotechnology paper that used a mutant MspA nanopore with phi29 DNA polymerase to achieve single-nucleotide resolution DNA reading.

• Web security concerns were raised regarding WebGL and browser technologies, highlighting the potential for exploits that could leverage GPU computing through browsers to create "zombie botnets."

• Creative web development techniques were shared, including methods for embedding HTML within PNG images and minimalist JavaScript implementations like a 20-line Castle Wolfenstein game.

• Participants exchanged resources on JavaScript development frameworks, particularly Backbone.js organization using modules, with debate about the utility of scaffolding tools like Yeoman, Bower, and RequireJS.

• A biohacking concept involving wolf spiders and potential life extension was shared (http://www.questsin.com/2012/09/wolf-spider-biohacking-hack.html).

• A detailed technical discussion covered laser etching projects using 405nm laser diodes, variable power supplies, and Blu-ray writer optics for precision etching of silicone.

• The group noted that Next Generation Sequencing (NGS) data processing typically requires cloud computing resources for alignment, with some institutions lacking sufficient computing capacity for this work.