# Key Points from IRC Discussions

• A Frassetto study was discussed that challenges conventional calories-in/calories-out models, showing sedentary non-obese people losing weight despite consuming over 5,000 calories daily.

• Copenhagen Suborbitals released CAD files for their space capsule designs, including Solidworks files for the Tycho Deep Space capsule, demonstrating open-source approaches in aerospace engineering.

• Participants debated 3D printing standards, noting that Makerbot's .thing format (a zip of .stl and .obj files) is insufficient for complex objects, with skepticism expressed about proposed XML-based standards.

• Kluyveromyces lactis and K. marxianus yeasts were discussed as efficient eukaryotes for protein production due to their GRAS status and the Crabtree-negative effect in K. lactis that prevents ethanol production in high glucose environments.

• Ben Krasnow's DIY liquid nitrogen generator was shared, which can be built for under $500, representing accessible approaches to advanced laboratory equipment.

• Alternative methods for accessing academic journals were suggested, including using community college credentials in wealthy areas, Reddit's r/scholar, and Chinese article sharing sites like i-nobel.com/bbs.

• A case study was referenced of a man with a brain tumor occupying 90% of his skull who remained functional though with diminished cognitive capacity, highlighting neuroplasticity and brain function questions.