# Key Points from the ##hplusroadmap IRC Discussion (October 1, 2012)

• OpenPCR bill of materials was shared (https://docs.google.com/spreadsheet/ccc?key=0AiaWH3PL_9CndG1GbmtSZ1I3aGdkQkE4YVhwZ3o5c1E#gid=0), providing resources for DIY PCR machine construction as part of the practical transhumanism focus of the channel.

• A bioscientist discussed research comparing gel electrophoresis buffers (TAE/TBE vs. TA/TB and sodium/lithium boric acid) with the goal of achieving single base pair resolution on agarose gels instead of using polyacrylamide.

• Participants explored aptamer technology for DNA analysis, describing them as loops of DNA that strongly hybridize to targets and require multiple iterations to develop through a process similar to genetic algorithms.

• The conversation compared different methods for SNP detection, debating whether aptamers, microarrays, or commercial sequencing would be most cost-effective and practical for DIY applications.

• A controversial DIY 3D-printed gun project was discussed after Stratasys reclaimed their printer upon learning it was being used to create firearms components, highlighting tensions between open hardware and potentially dangerous applications.

• The discussion touched on how potentially effective treatments like hookworm for Crohn's disease remain unevaluated because they aren't patentable, illustrating how patent considerations can create inefficiencies in medical research.