# Key Points from IRC Discussions on Transhumanism and Synthetic Biology

• Participants discussed DIY bio-printing technology, with specific plans to build a cell pick-and-place machine based on the GaudiLab algae picker design for creating neural tissue models, highlighting the ongoing democratization of advanced biotechnology tools.

• A significant technical challenge in tissue engineering was identified: creating extracellular matrices (ECM) and successfully transporting live cells into these structures, representing a frontier in DIY biohacking.

• The conversation explored synthetic protein interaction networks, particularly focusing on engineering protein kinase networks and the mechanisms by which kinases recognize their targets through electrostatic attraction and phosphate donation.

• G-protein coupled receptors (GPCRs) were discussed as versatile biological components with significant diversity, with participants noting they require eukaryotic expression systems (with yeast suggested as an easier alternative to mammalian cell lines).

• Participants explored the potential of using GPCRs as biosensors for detecting small organic molecules and pollutants, with specific interest in developing a serotonin receptor-based biosensor.

• The practical challenges of directed evolution with GPCRs were highlighted, with estimates suggesting such work might require "ten years in a well-equipped lab," indicating this approach remains primarily viable for well-funded research applications.

• Alternative approaches to biosensing were discussed, including using bacterial transcriptional regulators that respond to compounds of interest, which was noted as being more accessible than engineering GPCRs.