# Key Points from #hplusroadmap IRC Conversations

• DIY biohackers discussed gene therapy approaches using follistatin plasmids delivered via in vivo electroporation to enhance muscle growth, noting this method avoids both immunogenic rejection and the immunosuppression requirements of viral vectors by using normal human proteins.

• Participants explored advanced DNA synthesis technologies, including microfluidic PicoArray systems capable of synthesizing and assembling multiple DNA sequences simultaneously to create 10kb sequences, with discussions of purification methods to remove bacterial endotoxins.

• The San Diego DIYbio lab received city approval with a $1/year lease and utilities covered, representing significant progress in establishing community biohacking infrastructure.

• Technical discussions about microfluidics fabrication included using DLP (Digital Light Processing) technology to create molds, with plans to refine them using Focused Ion Beam technology to create nano-features like nanocapillaries for gel-free DNA separations.

• 3D printing techniques using photoinitiated polymers were analyzed, comparing UV-curable resins from different suppliers (Sigma Aldrich at ~$150/kg versus alternatives) and discussing the creation of thin polymer layers (~400nm) for microscale fabrication.

• A novel approach to reduce DNA synthesis errors was proposed using nanochannel technology to physically separate growing DNA molecules from active chemistry, protecting early-added nucleotides from damage during subsequent synthesis steps.

• Tactus Technology's microfluidic-based tactile interface that creates physical buttons on touchscreens was discussed, though with some skepticism about practical applications.

• The intersection of DIY biology with traditional hacking was highlighted through discussions of using Arduino boards and 3D printing for lab equipment, demonstrating how maker technologies are enabling citizen science in biotechnology.