# Key Technical Discussions from #hplusroadmap

• Participants explored custom photolithography systems using Sedgwick3D technology, which can complete projects in under 20 minutes without traditional masks, representing a significant advancement for rapid prototyping.

• The group discussed microfluidic technologies in detail, particularly droplet storage methods, sharing academic papers and AutoCAD files to facilitate DIY implementation of these advanced techniques.

• DIY electroporation techniques for genetic transformation were examined, with specific technical parameters (1.5kV, 25μF, 200 Ohms) for transforming yeast with GFP plasmids, alongside discussions about obtaining materials from AddGene.

• Light-controlled microfluidic systems were a focus, with participants sharing research on optoelectrowetting for droplet manipulation and exploring potential applications using DLP projectors as affordable control mechanisms.

• The group evaluated approaches to creating DIY alternatives to expensive lab equipment, including flow cytometry, cell sorting technologies, and an Agilent Bioanalyzer equivalent for NGS library quality control.

• Technical specifications for microfluidic systems were shared, including the preference for inverted microscopes in microfluidics applications and the typical pressure requirements (5-10 psi) for driving fluid through microchannels.

• A collaborative knowledge-sharing initiative was mentioned regarding a cryonics wiki (bettercryonics.com), with debate about integration with existing transhumanist wikis to maximize contributor participation.