# Key Points from #hplusroadmap IRC Discussion (March 5, 2013)

• Researchers discussed electroporation techniques for cell manipulation, noting that buffer solution composition is critical—glycerol/DI water works better than PBS, as ionic strength can "cook" cells during the process, with sonoporation suggested as a potentially gentler alternative.

• Scientific paper access was a recurring theme, with participants sharing papers on C. elegans muscular forces, intestinal microbiome research, and various electroporation techniques, highlighting the ongoing challenges of accessing paywalled academic content.

• An anecdote about George Hotz (geohot) described how he hacked a door key reader at RIT by recording the FM signal from an officer's card swipe and replaying it through a modified headphone jack, demonstrating practical security vulnerabilities.

• Biohacking resources were shared, including a German book on garage genetic engineering ("Biohacking, Gentechnik aus der Garage") and an article from The Scientist about biology hacklabs, with mention of Chesapeake BioWorks selling biotechnology education materials.

• Technical web automation tools were discussed, with kanzure mentioning using PhantomJS for web scraping and visualization generation, while others inquired about Selenium WebDriver for similar purposes.

• Mozilla developer bkero explained Firefox's remote debugging capabilities, noting that while a command-line version of developer tools wasn't yet available, the remote debugging protocol could support building a curses interface communicating over a pipe.