# Key Points from #hplusroadmap IRC Log

• The conversation focused on neural recording techniques for Lymnaea stagnalis (pond snail) ganglia, with researchers discussing how to test whether neurons survive vitrification (a cryopreservation technique).

• A budget-friendly approach using the $99 SpikerBox kit from Backyard Brains was proposed as an alternative to expensive commercial multi-electrode array (MEA) systems for neural recording.

• The open-source NeuroRighter platform was highlighted as a potential hardware solution for multi-channel neural recording and stimulation experiments, with PCB designs available online.

• Eleitl outlined a methodical testing protocol for snail ganglia vitrification using cryoprotectants like M22 and VM-1, involving controlled chilling, rewarming, and diffusion-perfusion techniques.

• Ethical considerations in cryonics were discussed, particularly the controversial practice of "active shutdown" (beginning preservation while patients are still alive) and varying death pronouncement criteria across countries.

• L. stagnalis was noted as an ideal model organism due to its large neurons (up to 100μm diameter), which facilitate electrophysiological recording and the study of neural networks controlling specific behaviors.

• CERN's contributions to open hardware design tools were mentioned, including support for Icarus Verilog and KiCad development through their technical student program.

• A raster-to-SVG conversion tool called "derasterizer" was shared (http://svg.hipokrit.com/), which converts pixel blocks to SVG circles based on average pixel values, useful for laser cutting applications.