# Key Points from #hplusroadmap IRC Discussion

• A DIYbio lab in Carlsbad secured a $1 lease on a 6000 sq ft facility and committed to hosting 8 biotech startups, but faces funding challenges for equipment like power-hungry -80°C freezers, exploring revenue streams including teaching lab classes for community colleges.

• Yashgaroth described a personal project on "DIY human genetic engineering" using plasmids to produce desired genes/proteins, with particular interest in direct electroporation of human muscle tissue.

• Cory Tobin from Los Angeles Biohackers is working on a biological Haber process using Streptomyces thermoautotrophicus, a bacterium reportedly capable of fixing nitrogen in the presence of oxygen while growing on carbon monoxide or hydrogen and CO2.

• The team sequenced S. thermoautotrophicus (with approximately 78% GC content) but is redoing the sequencing due to sample contamination, and Cory is attempting to reproduce controversial research by purifying its nitrogenase using sepharose columns.

• A link was shared to an academic paper in Science magazine about injectable, cellular-scale optoelectronics with applications for wireless optogenetics.

• Other projects mentioned include "Project Skynet" focused on reverse engineering an in vivo neural network on a chip, and discussions about novel 3D printing materials like wood filament (Laywoo-D3).