# Key Technical Discussions from #hplusroadmap

• Slur.io was introduced as an anonymous marketplace operating over the Tor network with Bitcoin transactions through libbitcoin, enabling unrestricted data trading between buyers and sellers.

• Participants explored enzymatic DNA synthesis as a promising alternative to current methods, with proof-of-concept examples suggesting entire genomes could be produced for less than $1.

• The conversation highlighted a significant limitation in biotechnology: solid-phase protein synthesis can only produce approximately 70 amino acids, restricting the creation of larger proteins needed for many applications.

• Nonribosomal peptide synthetases (NRPS) were discussed as an alternative to ribosomal peptide synthesis, capable of incorporating hundreds of different building blocks beyond the standard 20 amino acids.

• Technical aspects of ribosome function were examined, including translation speed (approximately 22 tRNAs per second) and accuracy (error rates of 10^-3 to 10^-6), along with questions about how long ribosomes can pause while maintaining functionality.

• Electrochemical discharge machining (ECDM) was presented as a method for machining ceramics and glass, using techniques like platinum-wire cathodes in sodium hydroxide solution.

• The group discussed CRISPR/Cas9 technology with reference to a foundational paper on "A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity."