# Key Technical Discussions from #hplusroadmap Channel

• Participants discussed robust data backup strategies following a cryonics expert's data loss, emphasizing the importance of redundant systems like Ceph nodes across ISPs for critical technologies including Bitcoin wallets.

• The conversation explored computing architecture contrasts between traditional cache-based designs and embedded memory approaches like Parallella's, which eliminates cache in favor of direct memory access for better silicon utilization.

• Bitcoin governance was debated, particularly regarding Gavin Andresen's proposal to increase block size limits (starting at 8MB with doubling every two years), with concerns this might threaten Bitcoin's decentralization.

• Researchers shared advanced DNA visualization techniques, criticizing simplified representations and promoting more accurate molecular models that show proper atomic detail.

• The channel discussed building an open-source DNA synthesis system using modified inkjet printer technology, specifically Epson F057020 print heads capable of dispensing 4-10 picoliter droplets suitable for oligonucleotide synthesis.

• Technical specifications for a "molecular printer" were explored, including requirements for nitrogen-filled chambers, FPGA controllers, and regular maintenance cycles to maintain coupling efficiency during DNA synthesis.

• Participants examined DNA assembly techniques like Gibson Assembly for joining multiple DNA fragments to create larger constructs (over 100 kbp), and discussed automation challenges for a complete synthesis pipeline.

• Resources were shared on atomic force microscopy (AFM) for biological applications, including papers on single-molecule reconstruction and video tutorials for implementing AFM in biology research.