# Key Points from Transhumanist IRC Discussion

• Researchers debated DNA synthesis technologies, comparing inkjet-based DNA microarrayers against electrochemical CMOS array systems (like CustomArray/Azco's $1650 chip), with discussions about error rates, cost efficiency (as low as $0.00001 per nucleotide), and the technical challenges of scaling microarray synthesis.

• The group explored advanced DNA retrieval methods including "Sniper Cloning" (using pulse lasers for non-contact retrieval of DNA beads) and Illumina's patterned flow cell technology, which uses surface tension and reaction kinetics to ensure single DNA sequences per well.

• Participants discussed control systems for high-precision DNA synthesizers, debating whether FPGAs, microcontrollers, or LinuxCNC would be optimal for real-time control of systems requiring coordination of motion control and printhead firing at rates exceeding 100 million drops per second.

• There was significant interest in developing low-cost DIY photolithography systems using Blu-ray optical components to achieve sub-micron precision, with applications in microfluidics and DNA synthesis if traditional phosphoramidite chemistry approaches proved challenging.

• The conversation covered single-molecule isolation techniques, including nanopores, proton pumps, and piezoelectric injection systems for isolating individual molecules in subfemtoliter droplets, with references to biological membrane-bound proteins as models for synthetic applications.

• A DNA synthesis machine with 1.2 million spots per chip was offered to biohacker spaces (including CounterCulture Labs), prompting discussions about maintenance requirements and potential alternative applications for the technology if repurposed.