# Key Points from #hplusroadmap IRC Discussion

• Participants discussed tDCS (transcranial direct current stimulation) research related to lucid dreaming, with resources shared from a collection at diyhpl.us focusing on neuromodulation techniques.

• The conversation included detailed technical discussion of DNA synthesis chemistry, particularly the phosphoramidite method, covering silane bonding, washing steps, and challenges in oligonucleotide synthesis on solid supports.

• Cambrian Genomics' DNA synthesis technology was examined, which utilized laser-based selection of DNA-containing beads after quality control via PCR and sequencing, though the company was reportedly facing liquidation following its CEO's suicide.

• Several innovative approaches to genome synthesis were explored, including "flood group" methods to combine DNA droplets without extensive pipetting, using techniques like wax printing/melting, electrostatic guides, and superhydrophobic surfaces.

• Participants discussed physical manipulation methods for DNA droplets, including infrared or heated pin needles to melt barriers and using gas (nitrogen/argon) via syringe pumps to move droplets.

• The Russian fox domestication experiment was referenced with video documentation shared (https://www.youtube.com/watch?v=0jFGNQScRNY), showing the long-term selective breeding project for domestication traits.

• Technical limitations of current gene assembly methods were highlighted, particularly the challenges of emulsion PCR techniques described as "a nightmare to develop" and the inefficiency of current thermocycler-based DNA synthesis approaches.