# Key Points from #hplusroadmap IRC Log

• Researchers discussed building a DIY DNA synthesizer using electrowetting technology (OpenDrop), which allows for manipulating droplets on a printed circuit board to combine oligonucleotides for DNA synthesis (http://www.gaudi.ch/GaudiLabs/?page_id=392).

• The group explored technical challenges in droplet-based DNA synthesis, including evaporation at high temperatures, cross-contamination risks, and alternative approaches like using LCD panels or light patterns (SCOEW) to control droplet movement.

• A user named FourFire described their research goal of using evolutionary algorithms to discover novel proteins that could increase genome stability and delay cellular death from oxygen deprivation, potentially useful for stroke or drowning victims.

• The proposed genome protection mechanism would involve creating proteins that could cross-check multiple copies of chromosomes and repair mutations when only one copy differs, potentially reducing mutation rates by orders of magnitude.

• Participants discussed using magnetic beads for wash steps in DNA synthesis, where a magnet could hold beads in place while surrounding liquids are removed, though there was debate about their practicality.

• The group shared resources on microfluidics including papers on inkjet patterned superhydrophobic paper and a finger-actuated digital microfluidics system that uses piezoelectric elements to convert mechanical energy into voltage pulses.

• One participant mentioned working on follistatin (a myostatin inhibitor) fused to an antibody Fc region, which extends the protein's serum half-life from 1 hour to 2 weeks and aids in clearance of target proteins.