# Key Points from #hplusroadmap IRC Discussion

• Political tensions within the transhumanist movement were evident through a petition against Zoltan Istvan's presidential candidacy and reports of members leaving the USA Transhumanist Party over concerns about "non-inclusive leadership."

• A significant longevity research paper was discussed that analyzed 4,698 single-gene deletion strains, identifying the tRNA exporter LOS1 as extending lifespan when deleted, with connections to dietary restriction and mTOR inhibition pathways.

• A participant detailed their DIY microfluidics setup for DNA synthesis, including plans to integrate spectrometry, peristaltic pumps, electrophoresis capabilities, and nanoporous membrane interfaces.

• Invivofectamine 3.0 Reagent was highlighted as an advancement in genetic delivery systems, using lipid nanoparticles for efficient in vivo delivery of siRNA and miRNA to mouse liver cells.

• Technical discussions covered low-cost Chinese CNC laser systems (NEJE) for photolithography applications, including specifications and firmware upgrade possibilities from GRBL 0.8 to newer versions.

• A systematic review on the effects of ketogenic diets on autism spectrum disorder was shared, representing interest in nutritional approaches to neurological conditions.