# Key Technical and Conceptual Points from #hplusroadmap IRC Discussion

• Researchers explored DNA nanopore sequencing challenges, specifically why translocation speed varies between bases, suggesting it may result from different electronic configurations, physical deformation of DNA, or strand unwinding rather than power supply issues.

• A novel selective breeding project was proposed for neural recording and stimulation that would select for brains with large-scale connectivity to easily-accessible regions, potentially eliminating the need for invasive deep brain probes.

• Participants discussed alternative power sources for brain implants, including glucose-powered devices and ultrasound-based transmission of power and data, addressing key challenges in brain-computer interface development.

• The conversation included detailed technical analysis of molecular "ticker tapes" that could use DNA/RNA polymerization for recording neural activity, with calculations about maximum recording rates based on cellular metabolism constraints.

• Researchers debated mechanical versus enzymatic DNA ligation techniques, including using AFM/STM tips for forced joining of DNA strands, with references to mechanosynthesis research and explanations of how enzymes facilitate ligation by manipulating electron distribution.

• A surprising discussion about hydrocephalus cases revealed that some patients with 95% of cranial space filled with cerebrospinal fluid maintain normal or above-average IQs, challenging fundamental assumptions about brain volume and cognitive function.