# Key Points from #hplusroadmap IRC Discussions

• IndieBio announced a significant funding package of $250,000 per startup for 15 companies in their next batch, with applications available through their website sf.indiebio.co, representing substantial investment in biotech innovation.

• Advanced protein engineering techniques were discussed, including TEV protease for self-cleavage of fusion proteins, BASIC assembly for DNA cloning, and theoretical possibilities of multi-ribosome protein synthesis for creating complex 3D structures.

• Researchers shared breakthroughs in brain research, including a Nature Neuroscience paper identifying 32 reproducible gene expression signatures representing distinct cell types in the human brain, and a promising Alzheimer's treatment using scanning ultrasound to remove amyloid-β plaques in mouse models.

• China's genetic research was highlighted, particularly CRISPR work at Yunnan Key Laboratory where scientists are augmenting neurological development in monkeys, sparking debate about ethical boundaries between disease correction and enhancement.

• A novel application of CRISPR technology was proposed: adapting mammals to cryogenic conditions by introducing genes for cryoprotectant synthesis into cells, potentially enabling a "cryonics tolerance gene therapy" for humans.

• DIY biohacking accessibility was discussed, with information about acquiring used DNA synthesizers for under $10,000 on eBay and resources including the DIYbio FAQ wiki (http://diyhpl.us/wiki/diybio/faq), reagent supplier p212121.com, and "the-odin" for biohacking supplies.