# Summary of #hplusroadmap IRC Discussion (Nov 19, 2015)

* A discussion was initiated about protocols for reduced-risk DIY gene therapy, including suggestions to test therapies in vitro on biopsied tissues and implement quality control to ensure specificity of RNA/DNA targeting.

* The conversation explored cryonics adaptation through gene therapy, with debate about whether selective breeding (potentially with millions of mice and increased mutation rates) or direct genetic engineering would be more effective for developing cryonics-compatible organisms within a reasonable timeframe (<20 years).

* Research on trehalose expression for desiccation tolerance in human cells was discussed, referencing a 15-year-old study where human fibroblasts expressing E. coli trehalose biosynthetic enzymes could be maintained in a dry state for up to five days with no detectable water.

* Recent advances in cell-penetrating peptides (CPPs) designed through molecular simulations were highlighted as a method for delivering trehalose into mammalian cells with high efficiency and low cytotoxicity.

* The group discussed the theoretical advantages of larger populations in genetic experiments, noting that increased parallelism allows faster searching of the genetic landscape, though this approach requires significant resources.

* A hackerspace design was mentioned featuring a grid of electrical outlets and data jacks throughout the facility (15'×15' grid) to accommodate frequent rearrangement of equipment and workspaces.