# Key Points from #hplusroadmap IRC Discussion

• Open-source biohacking tools were discussed, including 3D printable micropipettes that could be calibrated using milligram scales, and the potential for partially 3D-printed machines for screening small molecules against cancer cell lines.

• A novel drug discovery approach was proposed that would develop assays better predicting in vivo responses by screening molecules with known human outcomes to identify which assay best correlates with actual clinical results.

• Research on planarian flatworms revealed that transient perturbation of cell connectivity using gap junction blockers resulted in regenerated heads with shapes matching other species despite having the same genome, suggesting epigenetic control of morphology.

• A South Korean company (Sooam) successfully cloned a dog named Missy after 10 years of research at a cost of approximately $100,000 per clone, demonstrating advances in mammalian cloning technology.

• Researchers discussed NRF2 activators like curcumin and berberine as potential anti-inflammatory compounds, noting that despite bioavailability challenges, berberine performed similarly to metformin in diabetes trials and was potentially superior for treating polycystic ovary syndrome.

• A significant cryopreservation advancement from Oregon State University was shared, detailing a mathematical model for minimizing cell damage during vitrification through a two-step cryoprotectant exposure process, achieving 81% cell recovery compared to only 10% with conventional methods.

• Novel cancer treatment approaches were discussed, including research on oxygen cold-plasma therapy and the potential for molecules that could selectively up-regulate Nrf2 in healthy cells while down-regulating it in cancer cells.