# Key Points from ##hplusroadmap IRC Discussion (July 3, 2016)

• Researchers discussed nanopore-based DNA sequencing technologies, including a paper on MoS2 nanopores that improves signal-to-noise ratio by slowing DNA movement through viscous media, highlighting advances in sequencing technology.

• A significant conversation on DNA synthesis techniques covered modified inkjet technology, microfluidic channels, and photolithography on thin plastic sheets for creating high-density DNA arrays on flexible substrates.

• Participants shared information about a novel reverse transcriptase with error-correction capabilities ("Synthetic evolutionary origin of a proofreading reverse transcriptase"), which could substantially improve transcriptomics methods.

• The group explored DNA-based information storage technology, referencing papers on error correction in microchip-synthesized genes that achieved reduced error rates of approximately 1 in 8700 base pairs.

• Multiple CRISPR-related research papers were discussed, covering applications in oncolytic viruses, neuroscience, virus-inducible systems in insect cells, and AAV-mediated gene editing of retinal cells in vivo.

• A detailed exchange on plant tissue culturing techniques covered Murashige and Skoog Medium, improvised sterile environments, and proper laboratory techniques for avoiding contamination.

• The conversation highlighted laboratory safety concerns, particularly regarding ultracentrifuges, sodium azide handling, and the dangers of working with vacuum systems and organolithium compounds.