# Key Points from #hplusroadmap IRC Discussion

• Michael Greve committed $10 million to the SENS Research Foundation through his Forever Healthy Foundation, funding critical projects including Allotopic Expression of Mitochondrial Genes and glucosepane crosslink cleavage as part of a larger "Project|21" campaign targeting $50 million in private support for longevity research.

• Participants explored advanced DNA sequencing technologies using electrical conductance methods, discussing patents where capacitive labels attached to nucleotides produce measurable changes at nanoscale electrodes during polymerase activity.

• The concept of a "writozyme" (laser-inducible DNA synthesis system) was proposed as a potential solution for controlled DNA synthesis without reliance on expensive commercial reagents, representing an innovation in synthetic biology.

• The discussion highlighted E. coli's elegant solution to the replication paradox, where its 20-minute division time despite 40-minute genome replication is achieved through nested replication forks that begin replicating granddaughter genomes while daughter cells are still forming.

• Zinc sulfide (ZnS) powder was examined as a photocatalyst capable of producing hydrogen from water when illuminated, though its practical application is limited by high costs ($7,920-$33,075/kg) and the need for specific particulation with sulfur vacancies.

• Several patents on nucleotide technology were shared, including innovations in polymerase enzyme substrates with protein shields, molecular adaptors for dye conjugates, and recombinant polymerases with increased phototolerance.

• The thermodynamic limits of polymerization were debated, with participants questioning cellular replication constraints and identifying genome replication as a critical bottleneck that prevents uncontrolled "grey goo" scenarios in biological systems.