# Key Points from ##hplusroadmap IRC Discussion

• Kelu124 reported progress on the Murgen DIY ultrasound development kit, which has been redesigned to use breadboard modules with more off-the-shelf components including STM32 on Arduino-compatible WiFi boards, making it more accessible and cost-effective.

• Participants discussed advanced ultrasound techniques including synthetic aperture imaging, time-reversal methods, and the potential for single-element ultrasound in applications like biofeedback for stroke patients and body fat measurement.

• Ligandal's nanotechnology for precise delivery of nucleic acids (CRISPR, TALEN, RNA) to specific cells without viral limitations was highlighted as a potential breakthrough for more effective genetic medicine.

• The DEC2 genetic mutation, which allows people to function well on less than 5 hours of sleep, was discussed as potentially representing a "40% lifespan increase" in terms of active waking time—possibly the largest known longevity impact.

• Researchers shared information about phage-assisted continuous evolution (PACE), which uses bacteriophages to continuously evolve proteins or nucleic acids by linking phage reproduction to target molecular activity.

• A Nature paper on gamma frequency entrainment that attenuates amyloid load and modifies microglia was shared, suggesting potential applications for neurodegenerative disease research.

• "Sniper Cloning" technology was mentioned as enabling precise mapping and non-contact retrieval of target DNA clones from next-generation sequencing platforms, producing thousands of error-free synthetic DNAs in a single run.