# Summary of ##hplusroadmap IRC Log (Dec 31, 2016)

* A new CRISPR technology resource was shared (crispr.technology), along with research on efficient CRISPR/Cas9-mediated mutagenesis in Ciona that includes a one-step cloning-free protocol for sgRNA expression cassettes.

* Genome-wide association studies were discussed that identify loci influencing human facial variation (DCHS2, RUNX2, GLI3, PAX1, EDAR) and factors affecting facial and scalp hair features.

* Research on mechanosensory hair cells was shared, revealing they express two molecularly distinct mechanotransduction channels, including the PIEZO2 protein.

* A paper on storing digital information in living cells was discussed, with skepticism expressed about the proposed application of distributing encrypted pathogen genomes in cells.

* An alternative to fluorescence in optogenetics was highlighted: coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing.

* Research on DNA structure was shared, including direct observation of DNA knots using solid-state nanopores and DNA condensation in one dimension.