# Summary of #hplusroadmap IRC Discussion

• Research on gene therapy delivery methods was shared, including papers on nanoparticle-based vectors and extrasynaptic volume transmission in C. elegans connectomics that maps connections beyond traditional synaptic mapping.

• Multiple approaches for crossing the blood-brain barrier were discussed, including functionalized dendrimers with transferrin, rabies viral glycoprotein peptide complexes, and targeting agents like angiopep, lactoferrin, and chlorotoxin.

• Sonoporation was highlighted as a promising technique that uses microbubbles with ultrasound to temporarily disrupt the blood-brain barrier, creating micropores large enough for macromolecules to enter the brain.

• A needle-free blood drawing device called Hemolink by Tasso was shared, representing an advancement in less invasive medical testing technology.

• Skepticism was expressed about JangoBio's claims to reverse aging through parabiosis experiments, specifically criticizing their lack of cited mechanisms of action.

• A multilayer connectome of C. elegans was discussed, which extends beyond traditional synaptic mapping to include extrasynaptic connections via monoamines and neuropeptides, with a related GitHub issue for the OpenWorm project.