Session Start: Sun Oct 07 09:45:46 2007 Session Ident: #Chemistry [09:45] * Now talking in #Chemistry [09:45] * beta.PilotAge.com sets mode: +nt [12:33] * ll226 has joined #Chemistry [12:33] hi [12:33] * ll226 has quit IRC (Leaving) Session Start: Sun Oct 14 13:40:42 2007 Session Ident: #Chemistry [13:40] * Now talking in #Chemistry [13:41] * Taon has left #Chemistry [13:41] * Elephant has quit IRC (Leaving) [13:41] * Taon has joined #Chemistry [13:42] * Taon has left #Chemistry [13:42] * Disconnected Session Close: Sun Oct 14 13:42:15 2007 Session Start: Sun Oct 14 13:42:28 2007 Session Ident: #Chemistry [13:42] * Now talking in #Chemistry [13:42] * beta.PilotAge.com sets mode: +nt [13:42] * DrSturm has joined #Chemistry [13:42] * DrSturm has quit IRC [17:59] * DrSturm has joined #Chemistry [18:02] * second has joined #Chemistry [18:05] Hello Dr. Sturm... [18:05] I have a ? [18:06] In monday's lab we obtained a an image that was smeared, only half the columns can be seen [18:07] with that being the case, what should we do, as fas as the report is concerned? [18:07] I sent an e-mail but didn't have everyone's addresses, we actually did not finish the lab on Monday as we needed to do the desitometry. [18:08] I will hand out the data in lab on Monday so the report will be due the following Monday (10/22) [18:08] Ok. Thank you very much [18:12] Does the conversion of NAD --> NADH transfer 2e- [18:13] * DrSturm1 has joined #Chemistry [18:13] * DrSturm1 has quit IRC (Leaving) [18:14] yes [18:15] * agee has joined #Chemistry [18:15] * strssdout has joined #Chemistry [18:17] i'm justcurious, then why isn't the product of the conversion isn't NADH2 [18:17] NADH + H [18:17] what is the answer to Qs4 on work sheet 10?? [18:18] What is the question? [18:18] oh, i see now. that's exactly what your notes say... i guess i just missed that. thank you [18:19] why a combined analysis of plasma glutamyl transpeptidase and alanine aminotransferase is desirable for diagnosing hepatitis in a patient? [18:21] Glutamyl transpeptidase diagoses liver disease in general, Ala aminotransferase is specific for hepatitis [18:23] oh ok thanks. also why histidine is found in the active site of many diverse proteases? [18:23] for its ability to donate/accept protons [18:23] * second has quit IRC (Leaving) [18:25] what is competitive inhibitor? I'm still confused on that... [18:26] An ihibitor that competes with the substrate for the active site on the enzyme [18:29] is tomorrow's lecture going to be on the exam as well?? [18:30] yes [18:31] ok thanks Dr. Sturm :) [18:31] * agee has quit IRC (Leaving) [18:35] * Olivia has joined #Chemistry [18:36] * k9luv has joined #Chemistry [18:37] Do we need to know/trace the movement of e- in FAD? [18:38] Yes [18:39] Lab Question: For wednesday's lab, there are only 4 peaks in the SPE scan, but in the data table we have 5 different types of proteins. HOw do we know which protein is which? Do we use the range relative %? [18:40] Okay, that's where I'm confused: I see where 2 H's come from to bond to the N; but where do the other H's come from for the C's that lost the double bond? [18:41] Use the relative %'s [18:42] FADH2 is without double bonds, FAD i with double bonds [18:43] * sharpie has joined #Chemistry [18:44] Do we need to know the RDA's of the water-soluble vitamins? [18:44] Do we need to know the essential amino acids for the exam? [18:45] How do we define protein quality? Does it have to be an ideal protein and have good biological value? [18:45] No RDA's [18:45] No essential amino acids [18:46] A protein that is easily digested and contains all of the essential amino acids [18:46] Do we need to know the 8-step mechanism of Chymotrypsin action? [18:51] Are hypervariable regions=CDR's? [18:52] You need to understand the catalytic triad and the roles for Ser, Asp, His [18:52] Yes, hypervariable regions are CDR's [18:55] What does "TPP", the coenzyme for Thiamin, stand for? [18:56] Thiamin diphosphate [18:56] Would you define transition state as the state of highest energy in a rxn? [18:58] Yes, the transition state occurs at the top of the Ea peak [19:01] * k9luv has quit IRC (Leaving) [19:04] * Hb has joined #Chemistry [19:04] * sharpie has quit IRC (Leaving) [19:13] * Hb has quit IRC (Leaving) [19:27] * Olivia has quit IRC (Leaving) [19:30] * sp has joined #Chemistry [19:31] * kchap has joined #Chemistry [19:32] Is our quiz on Monday's lecture? [19:32] The quiz is on ser proteases [19:34] ok [19:35] Q. on worksheet 9 question #5 I would like to why Vmax is decresing and km is increasing in the non competitive inhibitor [19:40] * sharpie has joined #Chemistry [19:40] The inhibitor "drags" on the enzyme reducing it's maximum rate, the change in shape from the non-competitive inhibitor causes Km to increase (the affinity of the enzyme for its substrate to decrease) [19:41] similar topic, will Vmax also go down [19:44] for a non-competitive inhibitor Vmax decreases [19:45] thank you [19:46] * sharpie has quit IRC (Leaving) [19:46] on worksheet 10 question #2 I can't find the isozyme pattern for liver in the creatine phosphokinase or LDH [19:50] Don't worry about the liver isozyme just the heart isozymes [19:51] Thank you [19:54] on worksheet 11, #2 why is histidine found in the active site of many diverse proteases? [19:54] is it because it can donate and accept protons? [19:56] Worksheet #12 in the part of the vitamins of carbohydrate & nitrogen metabolism I'm having problems with Q. 1 &2 [19:57] yes, because it can donate/accept protons [19:57] thanks [19:59] The naswer key for Worksheet #12 will be posted in the glass case tomorrow AM [19:59] Any last questions/ [19:59] Thank you [19:59] * sp has quit IRC (Leaving) [20:00] * strssdout has quit IRC (Leaving) [20:00] * DrSturm has quit IRC [20:00] * kchap has quit IRC (Leaving) [20:01] * Disconnected Session Close: Sun Oct 14 20:01:33 2007