| --- Log opened Wed Apr 11 00:00:30 2012 | ||
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| diginet | is protein size generally inversely proportional to rate of expression? | 00:07 |
|---|---|---|
| yashgaroth | generally, yes | 00:08 |
| kanzure | why am i awake? | 00:14 |
| kanzure | and what is this http://www.youtube.com/watch?v=YwnM3eMh3Q8#t=1910 | 00:14 |
| kanzure | the whole fist of the north star? | 00:14 |
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| diginet | I'm worried that the expression of my protein will be too low to be usable | 00:31 |
| yashgaroth | there are ways around that...titin gets expressed all the time | 00:32 |
| diginet | lol, titin | 00:32 |
| diginet | doesn't titin have an absurdly low half-life, like 2 hours or something? | 00:33 |
| diginet | what are the ways around that? just using a strong promoter? or are there others? | 00:33 |
| yashgaroth | most of your problems are due to the size of the mRNA | 00:33 |
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| yashgaroth | more likely to get cleaved somewhere, slower to export from the nucleus | 00:34 |
| diginet | how does one rectify that? | 00:35 |
| yashgaroth | you can optimize a number of sequence features on the mRNA, though it's likely the spider has already evolved that | 00:35 |
| yashgaroth | also that 2 hour figure for titin is how long it takes to translate, not its half-life | 00:36 |
| diginet | ohhh | 00:36 |
| diginet | wow, that's looooong | 00:37 |
| diginet | I wish there was more research in the way of in vitro expression | 00:37 |
| yashgaroth | there's plenty, but in vitro expression sucks unless you've got some super-toxic protein | 00:38 |
| yashgaroth | also, the glycosylation and chaperone proteins don't usually work as well in vitro, if at all | 00:38 |
| diginet | true | 00:39 |
| yashgaroth | your main problem will be isolating a stable cell line with high expression | 00:40 |
| diginet | if you had to guess, for a 300 kDa protein, what kind of yields could I expect per liter, for a batch reactor? | 00:40 |
| yashgaroth | oh man there's way too many variables | 00:40 |
| diginet | I figured :( | 00:41 |
| diginet | what are the most important one? | 00:41 |
| diginet | *ones | 00:41 |
| yashgaroth | if you had all the money of big biotech, and if the protein doesn't interfere with the cells during/after its synthesis... | 00:41 |
| diginet | (generally speaking) | 00:41 |
| diginet | go on. . . | 00:41 |
| yashgaroth | stability of the protein after secretion is a major one | 00:42 |
| yashgaroth | if it immediately gets eaten by the cells then you're screwed | 00:42 |
| yashgaroth | so silk might fare well in that regard | 00:42 |
| diginet | it should | 00:42 |
| yashgaroth | that's part of why antibodies are so easy to grow - cells have FcRn receptor that spits them back out when they get ingested | 00:43 |
| yashgaroth | so you get baller yields | 00:43 |
| diginet | one thing that's bothered me is that I can't find any realiable information on the solubility of the dope | 00:43 |
| diginet | that's good | 00:43 |
| yashgaroth | I do wonder how the silk proteins are stored in the glands | 00:43 |
| yashgaroth | but generally the interior of a gland is a lot easier to modify (for an organism) than an entire flask of cell culture media | 00:44 |
| diginet | what do you mean? | 00:44 |
| yashgaroth | if they need to be kept at high pH or something, that's fine for cells that have a side that's kept exposed to normal blood | 00:45 |
| yashgaroth | the interior of the gland can have all sorts of weird conditions to store silk monomers in | 00:45 |
| yashgaroth | but at least part of the cell needs access to normal blood/media to keep it alive | 00:45 |
| yashgaroth | when your entire flask resembles the interior of said gland, the cells might not like it | 00:45 |
| yashgaroth | but I have yet to research what conditions the 'dope' is kept in, after being excreted | 00:46 |
| diginet | oh yeah, good point | 00:47 |
| diginet | how hard is it to sort proteins by size? I mean the silk proteins I gather would be significantly larger than most any others | 00:48 |
| yashgaroth | sort, in what sense | 00:49 |
| yashgaroth | the typical method of protein analysis is to run them through a gel by electricity, whereby the heavier proteins will migrate slower | 00:49 |
| diginet | err, seperate I guess | 00:50 |
| yashgaroth | if you don't go with affinity purification, size exclusion could indeed be the easiest way | 00:50 |
| diginet | can size different be used as a means to efficiently purify a protein? | 00:50 |
| diginet | ah | 00:50 |
| diginet | so affinity would be easier? | 00:50 |
| yashgaroth | not necessarily | 00:51 |
| diginet | constructing the collumn seems like it would be a pain | 00:51 |
| yashgaroth | nah | 00:51 |
| diginet | oh it isn't? | 00:51 |
| diginet | hmmm | 00:51 |
| yashgaroth | if you can get the specialized resin and a tube | 00:51 |
| diginet | sounds expensive | 00:51 |
| yashgaroth | yeeeeeah | 00:52 |
| diginet | well how do you get the protein out of the matrix? | 00:52 |
| yashgaroth | they come out eventually | 00:52 |
| yashgaroth | size exclusion works with a non-interacting resin that has nanometer size pores all over it | 00:52 |
| diginet | sounds expensive as well | 00:52 |
| diginet | :( | 00:52 |
| yashgaroth | the smaller proteins tend to get caught in said pores more easily, so they run slower | 00:52 |
| diginet | what about ammoniun sulphate precipitation? | 00:53 |
| diginet | *ammonium | 00:53 |
| yashgaroth | you can certainly try, hard to say how the silk would react | 00:53 |
| diginet | I read reports of it working | 00:53 |
| diginet | would that be easier, or more importantly, cheaper? | 00:53 |
| yashgaroth | it's a solid method, sure | 00:53 |
| diginet | (specifically with spider silk that is) | 00:54 |
| yashgaroth | oh way cheaper, if it works | 00:54 |
| diginet | ahhh | 00:54 |
| yashgaroth | silk proteins strike me as not having the highest solubility, which works both for and against you | 00:54 |
| diginet | In one paper, which produced the proteins in E. Coli, the cells were lysed and "acid precipitated" | 00:55 |
| diginet | any idea what that means? they claimed that the silk proteins were the only ones solluble in the acid | 00:55 |
| yashgaroth | it means they acidified it until everything but the silk proteins dropped out, yeah | 00:56 |
| diginet | the process I've seen that seemed to be the most promising was to heat up the mixture to 90 C, which denatures most of the proteins (but not the silk) and then precipitate it via ammonium sulphate | 00:56 |
| diginet | hmm, that sounds like a possible option | 00:56 |
| yashgaroth | you should really try collecting a bunch of webs and seeing what conditions you can use to purify | 00:57 |
| diginet | oooohhhh, good idea | 00:57 |
| diginet | I could dissolve the proteins, mix them with some other stuff, and try to get them back out | 00:57 |
| yashgaroth | pretty sure that enormous communal spider web was found in texas | 00:57 |
| diginet | haha | 00:57 |
| diginet | I feel pretty confident about being able to transform the yeast cells, what I'm nervous about, as previously mentioned, is the purification process, and the yield rates | 00:58 |
| yashgaroth | at least you can optimize the purification with some collected silk | 00:59 |
| diginet | true | 00:59 |
| yashgaroth | optimizing yield tends to involve plating out thousands of single cells and testing the colonies til you get one with respectable levels of expression | 00:59 |
| diginet | ahhh I see | 00:59 |
| diginet | that sound difficult\ | 01:00 |
| yashgaroth | usually under selection conditions as well | 01:00 |
| yashgaroth | yeah it is | 01:00 |
| diginet | I don't have a microscope | 01:00 |
| diginet | so | 01:00 |
| yashgaroth | everyone needs a microscope | 01:00 |
| diginet | hah, yes, I wish I had one | 01:01 |
| yashgaroth | surely some hacker space has one, if nothing else | 01:01 |
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| diginet | true true | 01:02 |
| diginet | I'm wondering, if I were to estimate my yields at say, 250mg/L, if I bought a 200 liter drum, that would be around 50g per batch | 01:03 |
| diginet | I wonder how far that goes | 01:03 |
| diginet | (in terms of the fiber) | 01:03 |
| diginet | I remember seeing a video on those guys trying to use goats to make the protein, they had a tiny vile of the purified protein that they said would produce a meter of fiber. I believe a single silkworm cocoon will generally yield around 600 m of silk, and it takes around, IIRC, 5000 cocoons to make a silk kimono | 01:05 |
| diginet | meaning you need 3 million meters of silk to make one kimono. Also meaning I'd need one million batches to make an equivalent garment out of spider silk | 01:06 |
| diginet | (given this process) | 01:06 |
| diginet | err | 01:06 |
| diginet | wat | 01:06 |
| diginet | bad math | 01:06 |
| diginet | I wonder how much silk was in that vile | 01:07 |
| diginet | I'd guess it was maybe around half a gram | 01:11 |
| diginet | meaning, each batch would yield 100 meters. thus putting my necessary batches at 30k | 01:12 |
| yashgaroth | better put on yer spider ranchin' hat, pardner | 01:12 |
| JayDugger | Good luck with that. Ever notice all animals raised on a ranch don't belong to the Predators' Union? | 01:14 |
| yashgaroth | naw man, we'll just, like...keep them super blazed on the chronic all the time, and they'll all get along dude | 01:15 |
| diginet | spiders have the endearing habbit of eating each other, so unfortunately I don't know if that's possible | 01:15 |
| JayDugger | Hmmm.... | 01:15 |
| yashgaroth | communal spiders seem to do ok | 01:15 |
| diginet | haha, communal spider rancher? | 01:16 |
| JayDugger | Yeah, you might find a particular breed or species that would serve. | 01:16 |
| JayDugger | The idea of intoxicated spiders might have more use than you'd think. | 01:16 |
| JayDugger | I remember years ago seeing photographs of spiders under the influence of hallucinogens. | 01:16 |
| yashgaroth | peep dis shit http://texasento.net/Social_Spider.htm | 01:16 |
| yashgaroth | yeah I think that was faked, at least the video version Jay | 01:16 |
| diginet | http://youtu.be/sHzdsFiBbFc | 01:17 |
| diginet | that comes to mind :P | 01:17 |
| JayDugger | That's quite possible. I don't remember where I read it, and I don't have a reference. | 01:17 |
| yashgaroth | but when you have a faux-tree structure, filled with a swarm of fruit flies...stick a colony of those spiders in and see what happens | 01:18 |
| yashgaroth | or a few really big flies, whatever makes the spiders decide to band together | 01:18 |
| diginet | yeah, then dissolve the webs, and reconstitute them as a single fiber | 01:19 |
| yashgaroth | bam | 01:19 |
| diginet | lol, spider farms | 01:22 |
| diginet | I think we can thus conclude that the only reasonable way of producing the silk in any appreciable amount is either farming or perhaps transgenic plants | 01:29 |
| yashgaroth | look we're either calling it ranching or rustling | 01:31 |
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| diginet | oohhh, look at this: apparently, if you insert the gene into the genome of the chloroplast, you get ridiculously high yields, like up to 30% total soluble protein | 01:32 |
| yashgaroth | plant cells are a bitch to lyse | 01:33 |
| diginet | sure but it would be worth it | 01:33 |
| yashgaroth | might be a way to selectively ultracentrifuge out the chloroplasts, but I don't work with plant cells | 01:34 |
| diginet | what do you work with generally? | 01:35 |
| yashgaroth | mammalian cells and e.coli | 01:36 |
| yashgaroth | plus pseudomonas or insect cells, depending on where my next job's at | 01:36 |
| diginet | what are the largest proteins one can produce from e.coli? | 01:37 |
| yashgaroth | I've never tried, but probably not much larger than whatever their largest naturally occurring protein is | 01:37 |
| diginet | I'm thinking plants are the most attractive option right now | 01:39 |
| yashgaroth | regardless, | 01:41 |
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| archels | http://trueskinfilm.com/ | 05:21 |
| archels | must... see... :O | 05:21 |
| phryk | swf? | 05:22 |
| phryk | sfw* | 05:22 |
| archels | yes | 05:22 |
| phryk | ah nice visuals | 05:23 |
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| archels | totally epic | 05:30 |
| archels | I can't wait to see it. :) | 05:30 |
| phryk | I'll have to watch the trailer later on. too lazy to plug in my headphones into another machine :P | 05:36 |
| archels | not much added value there, it's mostly the visuals. | 05:39 |
| phryk | the visuals are very interesting, I'll have to give it that much^^ | 05:39 |
| phryk | doesn't look like a full fledged professional production, but it very interesting. | 05:40 |
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| phryk | s/it// | 05:46 |
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| -!- Topic for ##hplusroadmap: biohacking, nootropics, transhumanism, open hardware http://gnusha.org/logs/ http://bit.ly/diybionews2 http://gadaprize.org/ http://groups.google.com/group/diybio | 06:16 | |
| -!- Topic set by kanzure [~kanzure@131.252.130.248] [Mon Feb 13 12:33:19 2012] | 06:16 | |
| [Users ##hplusroadmap] | 06:16 | |
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| -!- Channel ##hplusroadmap created Thu Feb 25 23:40:30 2010 | 06:16 | |
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| phryk | sylph_mako: you mean not actually projected into the physical world but some kind of AR layer? | 06:55 |
| sylph_mako | phryk, yeah. | 06:56 |
| sylph_mako | It just makes so much more sense. | 06:56 |
| phryk | Yup. | 06:56 |
| phryk | The formulation you just used… It's basically what I want to realize :D | 06:56 |
| sylph_mako | It offends me that the public still havn't caught on that that's how it's going to go. By the time we do get true holograms they'll seem /inconvenient/. | 06:56 |
| Vicarious | hi | 06:57 |
| phryk | I want to code a little service for AR devices that associates images (vector graphics if possible) with gps coordinates (including altitude) and orientation… | 06:57 |
| phryk | So you can go somewhere and the service will tell your device 'Hey over there, there's graphic X so load and display it' | 06:58 |
| phryk | One thing I haven't figured out is how I could make them disappear behind buildings | 06:58 |
| phryk | but that will prolly end up being the AR devices job, else I'd have to have a 3d map of the whole world and shitloads of calculations to do for every query… | 06:59 |
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| phryk | so the client on the ar device will prolly have some device to detect distances and calculate if the coordinate of the graphic is in front or behind the next object | 07:00 |
| sylph_mako | On the other hand, who wouldn't want to be able to see through buildings? | 07:00 |
| kanzure | sounds like a shared image cache | 07:01 |
| phryk | sylph_mako: you could have a noclip mode in your client of course :P | 07:01 |
| phryk | kanzure: cache sounds more volatile, but yes, something like that. | 07:02 |
| phryk | but my vision of it is that you can paint around and for instance mark special spots in your city. | 07:02 |
| phryk | you could have 'protected' tracks that only you and your friends can access for instance to mark good parkour routes. | 07:03 |
| kanzure | A. K. Barbey et al., An integrative architecture for general intelligence and executive function revealed by lesion mapping, Brain: A Journal of Neurology, 2012 | 07:04 |
| kanzure | http://dx.doi.org/10.1093/brain/aws021 | 07:04 |
| kanzure | http://brain.oxfordjournals.org/content/early/2012/03/05/brain.aws021 | 07:04 |
| kanzure | "Impaired performance on these measures was associated with damage to a distributed network of left lateralized brain areas, including regions of frontal and parietal cortex and white matter association tracts, which bind these areas into a coordinated system." | 07:05 |
| kanzure | "supporting their reliance upon a shared fronto-parietal network for the integration and control of cognitive representations" | 07:05 |
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| phryk | this is actually a further development of an earlier idea i had about geolocated content. | 07:06 |
| kanzure | why would i want my content inaccessible to me from other geolocations | 07:07 |
| phryk | Not like that. More like a pointer to some content. | 07:08 |
| phryk | The content would be accessible from everywhere. But you could place a pointer to a specific content of interest at a coordinate. | 07:08 |
| phryk | For instance you stand in front of a special building and in front of it is a pointer to the corresponding wikipedia article. | 07:09 |
| phryk | Or you're in front of a restaurant and you get a link to their menu and current specials | 07:09 |
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| kanzure | restaurant menus are never that high tech | 07:11 |
| kanzure | the only way to penetrate restaurants is through their point of sales system, sadly | 07:11 |
| kanzure | notice how yelp doesn't even have menus | 07:12 |
| phryk | kanzure: well not necessarily restaurants, but local pizza and other delivery services use services like pizza.de so they could just post a link to that ;) | 07:12 |
| kanzure | post a link to yelp? why | 07:13 |
| phryk | not yelp, to stuff like pizza.de | 07:13 |
| phryk | and pizza.de has the menus, since you can order from there ;) | 07:13 |
| kanzure | so, generally any programmer who has ever thought about AR has had your idea | 07:14 |
| kanzure | in addition to this, any programmer who has seen any AR demonstration video, has had your idea | 07:15 |
| kanzure | so how can you prepare for the influx of 10,000 programmers doing this exact project? | 07:15 |
| kanzure | openstreetmap prepared for this sorta | 07:15 |
| phryk | mhh | 07:15 |
| phryk | true. but i don't really care if it get's used much, it's nice playing around. | 07:15 |
| phryk | maybe i'll be motivated enough to invest a lot of time so that my implementation will be the 'best' one. | 07:16 |
| kanzure | well. there are already a few implementations out there. | 07:18 |
| kanzure | i was playing around with some. | 07:18 |
| phryk | can you point me to them? | 07:18 |
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| kanzure | https://github.com/haseman/Android-AR-Kit | 07:18 |
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| kanzure | http://github.com/zac/iphonearkit | 07:19 |
| kanzure | http://www.meetup.com/augmentedreality/ | 07:19 |
| kanzure | i don't know. that stuff. | 07:19 |
| kanzure | http://arq.freebaseapps.com/ | 07:19 |
| kanzure | http://www.androidkit.com/developing-augmented-reality-applications-for-android | 07:19 |
| phryk | that seems more like clients. I want to provide the service for those. | 07:19 |
| kanzure | just look at them | 07:20 |
| phryk | arquery seems more like what I want | 07:20 |
| kanzure | it wont hurt you | 07:20 |
| phryk | of course not, it's nice looking at different approaches to a topic :) | 07:20 |
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| delinquentme | ok serious question | 07:36 |
| delinquentme | anyone know how to stop google from putting up pics that a person has sent you | 07:37 |
| delinquentme | basically im reading emails and getting dirty pics when I shouldnt be seeing them | 07:37 |
| kanzure | in gmail? there's a setting to not display images from emails | 07:42 |
| delinquentme | i mean in the side bar | 07:43 |
| delinquentme | theres a fucking slide show in the side bar under the persons "google circles" | 07:43 |
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| kanzure | "As an aside, this is one of IBM's secret tricks - hardware accelerated money datatype. No other major vendor has this." | 07:48 |
| kanzure | http://www.ibm.com/developerworks/data/library/techarticle/dm-0801chainani/ | 07:48 |
| kanzure | wut | 07:48 |
| delinquentme | so signing out of google circles works for the time being =/ | 07:49 |
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| delinquentme | kanzure, sooo | 07:53 |
| delinquentme | i was thinking | 07:54 |
| delinquentme | the academic journals stuff would totally be something that google ventures could get behind | 07:54 |
| kanzure | ? | 07:56 |
| F71 | I wonder if diginet knows the golden orb weaving spider is like, ubiquitous in this area. | 07:57 |
| F71 | They used to scare the crap out of me with their bright colors and communal webs | 07:57 |
| F71 | Before the days of ubiquitous cameras I found a web with a finch caught in it. The big supporting silk was really strong. | 08:00 |
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| kanzure | ParahSailin__: hi | 08:11 |
| ParahSailin__ | hey | 08:11 |
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| Steel2 | kanzure | 08:15 |
| kanzure | what | 08:15 |
| Steel2 | I have a friend who's a (non bio-) chemist and is a little curious about what research in that field is specifically relevant to h+ stuff | 08:16 |
| Steel2 | they were looking at maybe doing polymers, but can you think of anything in specific? | 08:16 |
| kanzure | organic or inorganic guy | 08:16 |
| Steel2 | junior year, starting to look at grad schools | 08:17 |
| Steel2 | so either/or | 08:17 |
| Steel2 | more interest in inorganic | 08:17 |
| kanzure | organic chemistry: anything related to dna | 08:17 |
| kanzure | inorganic: anything related to diamondoid synthesis http://diyhpl.us/~bryan/papers2/nanotech/ | 08:17 |
| kanzure | just make sure he's not scared away by the "NANO NANO!!!" stuff | 08:17 |
| Steel2 | ...she >_> | 08:18 |
| kanzure | there's actually some good science going on there.. | 08:18 |
| Steel2 | it's my ex | 08:18 |
| Steel2 | also, isn't that more matsci than chem? | 08:18 |
| kanzure | wait. diamonds are organic | 08:18 |
| kanzure | nope, nvm | 08:18 |
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| Steel2 | diamonds aren't organic | 08:19 |
| Steel2 | they're carbon based but don't meet any of the other requirements | 08:19 |
| kanzure | huh? fertilizers are inorganic? | 08:20 |
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| kanzure | well i guess so | 08:21 |
| kanzure | so yeah, those are the two areas that come to mind. | 08:22 |
| kanzure | drazak: maybe you have better suggestions | 08:22 |
| delinquentme | kanzure, you're a linear string pot | 08:22 |
| delinquentme | Steel2, recruit em! | 08:23 |
| kanzure | i'm really just a bot http://heybryan.org/mac.html | 08:23 |
| Steel2 | delinquentme: Working on it | 08:25 |
| Steel2 | oh shit | 08:25 |
| Steel2 | I recognize a name in this irc channel | 08:25 |
| Steel2 | I think | 08:25 |
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| phryk | kanzure: heh, and did you meet him?^^ | 08:35 |
| kanzure | mac? yeah he's at nearly any conference i go to | 08:35 |
| phryk | Aah, okay :) | 08:36 |
| phryk | But you've never been to any of the CCC events in germany, have you? | 08:36 |
| kanzure | nope | 08:37 |
| joshcryer | how do we know mac isn't a bot too | 08:37 |
| phryk | kanzure: any particular reason for it? | 08:37 |
| kanzure | the twist is that everyone but me is a bot | 08:37 |
| kanzure | phryk: for not going to CCC? nope. i just haven't done it yet. | 08:38 |
| phryk | kanzure: If you do so, tell me :P | 08:38 |
| kanzure | in general i have stopped going to conferences where i am not speaking. but CCC, defcon, hope, might be interesting exceptions | 08:38 |
| Steel2 | kanzure: I'm at the same school as azonenberg, just released. | 08:38 |
| phryk | Seeing that you at least in some way associate with nick farr, you'd have a pretty easy way of getting there too, i think. | 08:38 |
| Steel2 | And we have a number of intersecting friends | 08:39 |
| Steel2 | released = realized | 08:39 |
| kanzure | Steel2: you should totally go be drinking buddies | 08:39 |
| Steel2 | talk about microstereolithography while drinking microbrews | 08:39 |
| Steel2 | :V | 08:39 |
| kanzure | phryk: yeah, i know nick a little | 08:39 |
| phryk | kanzure: I think he organizes a group-trip every year. | 08:39 |
| gedankenstuecke | phryk: are you going to ccc's sigint next month? | 08:41 |
| phryk | gedankenstuecke: i don't know, but hi there. | 08:41 |
| phryk | gedankenstuecke: what'll it cost / where is it? | 08:41 |
| phryk | the flyer is somewhere at home, only used for handling weed ^^; | 08:41 |
| gedankenstuecke | phryk: it's in cologne, dunno about the costs, but ticket sale will start today at 2200 UTC+2 | 08:42 |
| phryk | gedankenstuecke: this month i couldn't pay for the ticket… | 08:42 |
| phryk | my last money went into the ticket for the mayday… | 08:42 |
| gedankenstuecke | ic | 08:42 |
| phryk | I might get it organized though… | 08:42 |
| gedankenstuecke | my guess is that they will have tickets left (at least this is how it was in 2010 iirc) | 08:43 |
| phryk | cologne wouldn't be too far, either and I suspect that some other guys of the zone are going, too. | 08:43 |
| phryk | I think I could sleep at a friends place, too. | 08:44 |
| gedankenstuecke | uh, your at the warpzone right? | 08:45 |
| phryk | yes, i believe we have met one or two times. | 08:45 |
| gedankenstuecke | haha, i'm to bad with nicknames but somehow your's felt familiar :D | 08:45 |
| phryk | You're 'Bastian Greshake', right? | 08:46 |
| gedankenstuecke | yup | 08:46 |
| phryk | I don't know when you've been here the last time, but I'm colloqually know as 'the guy with the magnet' :P | 08:46 |
| gedankenstuecke | it's been a while since i moved to frankfurt for my master studies, but i think plaetzchen told me about "the guy with the magnet" a couple of times. and i'm still lacking mine, as finding a date with the piercer was harder than expected | 08:47 |
| phryk | But you have a date for it now? | 08:47 |
| phryk | I don't know about frankfurt, but there are piercers in mannheim, berlin and cologne doing it. | 08:48 |
| gedankenstuecke | nope, as i'm currently in tübingen for a job at the max planck institute there, but i'll get a date as soon as i'm back | 08:48 |
| gedankenstuecke | i already visited the guy in mannheim to get it implanted, but the american seller of his magnets had some shipping problems, so he hadn't have a single magnet left at the time i arrived :D | 08:49 |
| phryk | Ah sucks. You're talking about Marucs, right? | 08:49 |
| phryk | He seemed very able, even though I got mine done by Haworth. | 08:49 |
| gedankenstuecke | yeah :) | 08:49 |
| phryk | If they take pictures of it, let them give you them on the spot though. | 08:50 |
| phryk | Haworth told them not to give me my pictures -_- | 08:50 |
| gedankenstuecke | why's that? | 08:50 |
| phryk | No clue, I guess because of the anaesthetics… | 08:51 |
| gedankenstuecke | mh k | 08:51 |
| phryk | Also you might want to get in contact with rin, she'll like to get a few more experience reports :) | 08:51 |
| gedankenstuecke | i already follow her on twitter :) | 08:52 |
| phryk | Good ^_^ | 08:52 |
| Steel2 | rin who? | 08:52 |
| phryk | rinpaku | 08:54 |
| phryk | on the twitters | 08:54 |
| phryk | I'll be on my way to the warpzone now ;) | 08:54 |
| kanzure | gedankenstuecke: which max planck institute are you working at? | 08:54 |
| gedankenstuecke | phryk: hf and greetings to the other guys :) | 08:54 |
| phryk | Will relay. ttyl | 08:55 |
| gedankenstuecke | kanzure: i'm in this group right now: http://webdav.tuebingen.mpg.de/u/karsten/group/index.html?page=news | 08:55 |
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| kanzure | hrm.. alright "Graph-based Functional Classification of Proteins using Kernel Methods" | 08:58 |
| delinquentme | http://evolutionarypsychiatry.blogspot.com/2011/02/lithium-and-longevity.html | 08:58 |
| kanzure | "Representative Subgraph Sampling using Monte Carlo Markov Chain Methods" | 08:58 |
| delinquentme | anti-aging nootropic? | 08:58 |
| kanzure | gedankenstuecke: i spent a bunch of time in a graph theory lab, heh.. | 08:58 |
| kanzure | ooh ooh "ShapePheno: Unsupervised extraction of shape phenotypes from biological image collections" | 08:59 |
| gedankenstuecke | kanzure: i have no clue about graph theory, kernel methods etc. i'm just here to program a web-application :P | 08:59 |
| kanzure | gedankenstuecke: meh. just use networkx and you have 80% of the knowledge you need. | 09:00 |
| delinquentme | gedankenstuecke, howdeh. | 09:00 |
| kanzure | gedankenstuecke: what's the web app you're building? | 09:00 |
| gedankenstuecke | kanzure: basically an upgrade on what we've done with opensnp | 09:01 |
| kanzure | snpedia? | 09:01 |
| gedankenstuecke | enabling scientists to upload their genotype/phenotype-data no matter what species and enable gwas-computation in the cloud using the algorithms which have been developed here | 09:01 |
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| kanzure | crossbow? | 09:02 |
| gedankenstuecke | i think their algorithms are unpublished and i haven't seen them so far tbh | 09:02 |
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| delinquentme | OMG | 09:12 |
| delinquentme | someone BRING ME CAKE. | 09:12 |
| delinquentme | kanzure, whos the intern today? | 09:12 |
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| Urchin | do any of you confuse irc commands and unix commands | 09:26 |
| Urchin | ? | 09:26 |
| delinquentme | Urchin, nah sorry | 09:27 |
| delinquentme | I confuse tacos and taquitos often though | 09:27 |
| delinquentme | kanzure, I dont need to scrape elsevier do I | 09:35 |
| delinquentme | nm ill get their indexes bc I think we're working w slightly diff stuff | 09:35 |
| kanzure | well at least their site has bibtex export of an issue's citations/index, so take advantage of that | 09:36 |
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| kanzure | delinquentme: don't forget about http://diyhpl.us/cgit/pyscholar/tree/zotero-import | 10:14 |
| delinquentme | those are publishers right? | 10:16 |
| kanzure | these are zotero plugins for scraping pages and turning metadata into bibtex. also for grabbing pdfs from the pages. | 10:18 |
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| kanzure | hello malaimo | 10:43 |
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| F71 | here | 10:45 |
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| audy | http://i.imgur.com/0eQcO.png | 11:15 |
| audy | source: groupon for kidz advert | 11:15 |
| audy | e-m@le the footless transhumanist | 11:16 |
| F71 | what the | 11:20 |
| nmz787 | fenn kanzure: ping | 11:27 |
| kanzure | nmz787: pong | 11:27 |
| nmz787 | how fast do we need to cut again? | 11:27 |
| kanzure | fenn is probably away until friday night | 11:27 |
| nmz787 | ahh | 11:27 |
| nmz787 | ok | 11:27 |
| kanzure | nmz787: i'd like to keep it under 30 minutes per chip :P | 11:27 |
| kanzure | 10-12 minutes would be pretty great | 11:27 |
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| delinquentme | kanzure, http://www.elsevier.com/wps/product/cws_home/717248 | 11:30 |
| delinquentme | where bibtex | 11:30 |
| nmz787 | kanzure: so i calculate 1,000,000 drops, * 1.25 for overhead * 25 to get 31,250,000 microns, or 31,250 mm | 11:31 |
| nmz787 | kanzure: so 2000 mm/min is 15 mins | 11:31 |
| nmz787 | kanzure: 20:53 <@fenn> at 20kHz, maximum speed would be 3969 microns/s @ 16 microstep | 11:33 |
| nmz787 | which is 238.14mm/min | 11:33 |
| nmz787 | kanzure: thats 131 minutes | 11:34 |
| nmz787 | kanzure: that's with linuxcnc though | 11:36 |
| kanzure | delinquentme: access that through sciencedirect and use the "export citation" tool like in the phantomjs javascript i showed you | 11:38 |
| kanzure | nmz787: yeah, that does sound sorta slow | 11:38 |
| kanzure | how much space is that for a million drops? | 11:39 |
| kanzure | also the time it takes to pump out a million drops is probably astronomical? | 11:39 |
| nmz787 | depends on pump speed i guess | 11:40 |
| nmz787 | :/ | 11:40 |
| nmz787 | parallelize? | 11:40 |
| kanzure | we should really do the math on the pressure requirements | 11:40 |
| F71 | What about digital light solutions? | 11:43 |
| F71 | I think that's common in microfluidics. It's certainly highly paralell | 11:43 |
| kanzure | F71: then you have to wait for it to polymerize | 11:43 |
| F71 | depends on the polymerization speed | 11:43 |
| F71 | also, it's probably easier to make bricks of clever routing, as opposed to stacking layers with vias | 11:44 |
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| kanzure | F71: i bet we can get per chip fabrication time down to 10-20 minutes per chip with a laser | 11:45 |
| kanzure | with polymerization i'm not really sure this would work | 11:45 |
| kanzure | "The dominant rate limiting step is curing, and high temperature cures used to speed the curing process have adverse effects on the shape of the parts produced" | 11:47 |
| kanzure | "This thesis examines the PDMS cure process and presents a methodology to intelligently design faster cure processes without compromising the quality of parts produced. The first part of this thesis applies statistical mechanics to relate the time evolution of cure with the modulus of elasticity." | 11:47 |
| kanzure | http://dspace.mit.edu/handle/1721.1/61615 | 11:47 |
| kanzure | "Room temperatures very accurately replicated the dimensions of the master tooling, but required that PDMS sit undisturbed for two days while it solidified [19]." | 11:50 |
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| kanzure | "The recommended cure time for Dow Corning Sylgard 184 is 2 hours at 65C. Increasing the process temperature increases the cure rate, so this time can be reduced to 1 hr at 75C or 30min at 85C. Similarly, decreasing the process temperature to room temperature increases the cure time to two days." | 11:53 |
| nmz787 | yeah its easier to have pre cured PDMS blanks | 11:54 |
| nmz787 | simon replied to me | 11:54 |
| kanzure | i didn't get the email | 11:54 |
| nmz787 | forming a response onw | 11:54 |
| nmz787 | now | 11:54 |
| nmz787 | i noticed | 11:55 |
| kanzure | nmz787: precured pdms? i thought you cure it with the pattern projected over it | 11:55 |
| kanzure | oh you probably mean "it's easier to not do masked curing" | 11:56 |
| kanzure | i wonder if running the laser cutter with an fpga would be a better idea or not | 11:59 |
| kanzure | oh right. impossibly proprietary fpga tools. | 11:59 |
| nmz787 | no propeller would be fine | 12:01 |
| nmz787 | fenn didn't like the idea of writing an XYZ interpreter | 12:01 |
| nmz787 | doesn't seem like a terrible idea to me | 12:02 |
| nmz787 | i dont know about g-code, but it seems like XYZ positions for each step in a file on an 8gb SD card would be enough to do anything | 12:02 |
| nmz787 | XYZ, laser on/off | 12:03 |
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| kanzure | nmz787: i don't think it should be an sdcard | 12:14 |
| kanzure | take a look at some gcode from the web.. see what sorta things it should handle | 12:14 |
| delinquentme | http://www.elsevier.com/wps/find/bibliographicdatabasedescription.cws_home/422597/description#description | 12:18 |
| delinquentme | when companies have internal structure exposed to the internet | 12:21 |
| delinquentme | you can begin to get an idea of how ill structure their schema is | 12:21 |
| delinquentme | I really want to tweet this at one of the elsevier accounts | 12:22 |
| delinquentme | :D | 12:22 |
| kanzure | don't.. | 12:23 |
| kanzure | jesus man. don't do it. | 12:23 |
| kanzure | nmz787: thx for writing out that email | 12:24 |
| delinquentme | lol | 12:28 |
| delinquentme | but its so true | 12:29 |
| delinquentme | kanzure, is there any way to get traffic analytics for a website that I dont own? | 12:30 |
| delinquentme | like would I be able to tell when peak hours are for a website which I dont run? | 12:30 |
| kanzure | no. alexa is a scam. | 12:34 |
| delinquentme | haha realluuuuu | 12:35 |
| delinquentme | also http://pastie.org/3770073 | 12:36 |
| delinquentme | can I run a grep replace on that ^^ | 12:36 |
| kanzure | grep replace on what? | 12:36 |
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| kanzure | do you mean .gsub or sed? | 12:38 |
| delinquentme | ermm yeah | 12:41 |
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| delinquentme | oh wait its grep and awk that people use right? | 12:42 |
| kanzure | curl http://pastie.org/pastes/3770073/download | sed -i 's/puts/print' | 12:42 |
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| delinquentme | kanzure, whut is dis black majik | 12:52 |
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| F71 | My interpreter borked. What is that? | 13:05 |
| kanzure | ruby | 13:05 |
| kanzure | delinquentme: bash | 13:05 |
| F71 | I know that | 13:05 |
| kanzure | F71: related to http://diyhpl.us/~bryan/irc/ezproxy.py | 13:06 |
| diginet | you know what drives me nuts? GMO fearmongers. Granted there are a few legitimate concerns, like unforseen consequences for the environment. However, most people I hear complaning about them think we're all going to be poisoned by them or something idiotic like that. | 13:09 |
| delinquentme | diginet, me too! | 13:09 |
| Steel_ | diginet, did you see Amanda Stoel's post on facebook? | 13:11 |
| diginet | no, I didn't? Who's that? | 13:11 |
| Steel_ | one of the sing network people | 13:11 |
| Steel_ | she's posted some un-backed up gmo stuff in the past, which I hit with actual articles and she took down | 13:11 |
| diginet | hahaha | 13:11 |
| Steel_ | like anyone who says shit about monsanto and bees | 13:11 |
| Steel_ | .... | 13:11 |
| Steel_ | monsanto doesn't even produce the type of insecticide that has been linked with CCD | 13:12 |
| Steel_ | that's all Bayer's stuff | 13:12 |
| diginet | Like I said, there is some genuine cause to be concerned, with things like non-seed-bearing plants cross-polinating, but that is NOT what people these tools are usually whining about | 13:12 |
| Steel_ | and honestly, even that's not a huge worry | 13:13 |
| Steel_ | terminator genes breed themselves out of existence | 13:13 |
| kanzure | diginet: you should eat some GMO food on DNA day this year | 13:13 |
| Steel_ | not to mention monsanto I believe pulled them from shelves (I don't know for sure) due to backlash | 13:13 |
| kanzure | diginet: https://groups.google.com/forum/?fromgroups#!topic/diybio/FdpzU6-T6KE | 13:13 |
| diginet | here's the thing: transgenic pesticides, or DDT? Yeah, I'll take the former | 13:15 |
| Steel_ | Also, I'm trying to make a connection with Alex Knapp, Forbes' head tech writer, who is about to start writing on the singularity | 13:15 |
| diginet | Honestly, do anti-GMO people WANT thousands of people to starve? Because that's what would happen if they were banned | 13:15 |
| Steel_ | he said he might take my advice and point out in one of his articles the difference between singularity and transhumanism | 13:15 |
| Steel_ | he's the one who wrote the article pointing out kurzweil's inaccuracies | 13:16 |
| diginet | oh good | 13:16 |
| diginet | then I like him :) | 13:17 |
| diginet | kurzweil is an arrogant douche, but that's just me | 13:17 |
| kanzure | no that's everyone's opinion of ray | 13:17 |
| kanzure | that's the general consensus, i mean. | 13:17 |
| kanzure | i don't know why you think otherwise? | 13:17 |
| diginet | I see | 13:17 |
| diginet | well there seems to be a lot of people who take him seriously | 13:17 |
| kanzure | who? | 13:17 |
| Steel_ | people who aren't already in the culture, or who are lured by scientism instead of science | 13:18 |
| nmz787 | I would love to find some more on this kind of stuff: | 13:18 |
| nmz787 | http://arxiv.org/PS_cache/arxiv/pdf/1104/1104.3113v1.pdf | 13:18 |
| nmz787 | http://www.homeopathy.org/files/LucMontaigner2009.pdf | 13:18 |
| delinquentme | nmz787, what about it | 13:18 |
| delinquentme | what makes it desireable? bc isnt that a google search away? | 13:19 |
| kanzure | nmz787: "muck lower frequencies" ? | 13:19 |
| nmz787 | if thats true, it'd be cool to set up some bacterial oscillators between culture flasks | 13:19 |
| nmz787 | delinquentme: its hard to find anything about bacteria and radio waves | 13:19 |
| diginet | are we taking homeopathy seriously here? tell me no | 13:19 |
| F71 | Oh, diginet | 13:19 |
| diginet | F71: hmm? | 13:19 |
| nmz787 | diginet: it was published in Interdiscip Sci Comput Life Sci | 13:20 |
| delinquentme | nmz787, "bacteria" AND "radio" | 13:20 |
| delinquentme | ? | 13:20 |
| F71 | You know Golden Orb Weaving Spiders are indigenous to our area, right? | 13:20 |
| nmz787 | delinquentme: yes, i know how to search google | 13:20 |
| F71 | Like, I used to run into their communal webs when I was little and scare the crap out of myself | 13:20 |
| diginet | F71: yes, but I decided to go with L. hesperus (black widow) because the genes are already sequenced | 13:20 |
| delinquentme | ... | 13:20 |
| F71 | How does black widow silk compare to GOWS? | 13:21 |
| diginet | nmz787, doesn't really matter who publishes it, anyone who actually thinks that water has "memory" is a crank | 13:21 |
| diginet | about the same | 13:21 |
| diginet | it might be slightly stronger | 13:21 |
| kanzure | diginet: there doesn't seem to be anything about memory-water in that arxiv paper | 13:21 |
| kanzure | dunno what you're talking about | 13:22 |
| F71 | how conductive is it? | 13:22 |
| diginet | probably not very much, why? I've heard of people implanting gold in silk to make it conductive | 13:22 |
| nmz787 | diginet: its specifically talking about DNA in the water | 13:23 |
| diginet | just to be clear, you guys ARE saying homeopathy plasubily exists, and isn't just naturopathy woo, or placebo? | 13:23 |
| diginet | *plausibly | 13:23 |
| nmz787 | diginet: nothing of the sort, I'm interested in the claim that plasmids can resonate EM radiation | 13:23 |
| F71 | no, thermally conductive | 13:24 |
| F71 | GOW spider dragline silk is more conductive than copper | 13:24 |
| diginet | oh oh, actually I don't know. That's a very good question | 13:24 |
| F71 | thermally, I mean | 13:24 |
| diginet | Where do you find that data? | 13:24 |
| diginet | *did | 13:24 |
| F71 | I'll grab some papers | 13:24 |
| diginet | thanks | 13:24 |
| diginet | nmz787, oh good! hahah | 13:25 |
| diginet | sorry for the misunderstanding | 13:25 |
| diginet | I was worried there for a moment | 13:25 |
| nmz787 | :D nononono | 13:25 |
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| F71 | http://onlinelibrary.wiley.com/doi/10.1002/adma.201104668/full | 13:26 |
| F71 | need the paper still, ffff | 13:27 |
| F71 | but it gets 20% more conductive when stretched to 20% | 13:27 |
| F71 | it scales conductivity very linearly | 13:27 |
| F71 | one could use it to make adjustible thermal conductivity | 13:27 |
| nmz787 | F71: so what about modifying spiders, i.e. just removing their legs surgically/genomically | 13:28 |
| F71 | and fabric made from it would conduct heat better than copper | 13:28 |
| F71 | that'd be a good idea | 13:28 |
| diginet | F71: I'm in a bit of a quandry as to how I'm going to actually make the protein. I figured that even with optimistic yields, it would take on the order of 20k batchs in a 55-gallon drum to make one pound of silk | 13:28 |
| kanzure | nmz787: that sounds harder than protein purification ;) | 13:28 |
| Moksparagus | nmz787: why not grow the silk glands ex-vitro/ | 13:28 |
| F71 | yeah, it's the glands I'm wondering about | 13:28 |
| diginet | Moksparagus, I have a feeling that's a lot harder than it sounds | 13:28 |
| nmz787 | Moksparagus: that sounds alright if you can get glands to grow on their own easily from the egg | 13:29 |
| F71 | I havent seen any papers on taking the raw monomers and 'spinning' them | 13:29 |
| nmz787 | F71: so you don't know if you can produce in liquid or not? | 13:29 |
| diginet | and actualyl in vitro means outside of the organism, so ex vitro means the opposite of what you think | 13:29 |
| diginet | F71, really? I have | 13:29 |
| nmz787 | in vitro means in glass | 13:29 |
| Moksparagus | really? | 13:29 |
| diginet | I have about five or so | 13:29 |
| * Moksparagus just makes up words without understanding them. | 13:29 | |
| F71 | cool, I'd like to see those | 13:29 |
| diginet | nmz787, oh right, of course, as in vitreous | 13:30 |
| diginet | hold on | 13:30 |
| diginet | I'll look for them in a minute | 13:30 |
| diginet | but anyhow, the best option I could think of was plants' | 13:30 |
| diginet | by inserting the gene into chloroplasts, you can reliably yield at least 10% total protein content | 13:31 |
| F71 | just gluing a spiders in arrays, ass-up, and letting a machine slowly draw thread sounds elegant | 13:31 |
| kanzure | delinquentme: curl http://pastie.org/pastes/3770073/download | sed -i 's/puts/print' | 13:31 |
| Steel_ | wait, so who's genetically modifying spiders here? O_o | 13:31 |
| diginet | F71: feeding them? | 13:31 |
| kanzure | Steel_: the twist is that we're all spiders | 13:31 |
| kanzure | Steel_: diginet is our resident spiderman | 13:31 |
| F71 | crickets | 13:31 |
| delinquentme | kanzure, theres just lots going on here | 13:31 |
| diginet | I would feel really guilty about doing that though :( | 13:31 |
| delinquentme | but anyways | 13:31 |
| F71 | they're crickets | 13:31 |
| kanzure | delinquentme: yeah, but i think people can help possibly better than i can :) | 13:31 |
| diginet | F71, sure but how would they wrap the crickets up | 13:31 |
| diginet | no, the spiders | 13:32 |
| kanzure | bash piping is pretty simple | 13:32 |
| delinquentme | 's/puts/print' puts needs to be a big ass long strinf | 13:32 |
| F71 | they get a free lunch | 13:32 |
| delinquentme | string** or that curl input | 13:32 |
| F71 | It's not much different than a dairy cow | 13:32 |
| kanzure | delinquentme: like what | 13:32 |
| F71 | except less neurons | 13:32 |
| diginet | I hate milk though, bleh | 13:32 |
| delinquentme | like the contents of that pastie | 13:32 |
| diginet | hah | 13:32 |
| nmz787 | seems like you could knock out some neurons too | 13:32 |
| kanzure | delinquentme: you want to replace the entire contents? | 13:32 |
| diginet | but where do I get the spiders from? | 13:32 |
| nmz787 | if all you want them for is their sex organs, silk organs, and digestive system (mouth to anus) | 13:33 |
| delinquentme | I need to replace that big ass string with another big ass string | 13:33 |
| Moksparagus | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1409362/pdf/immunology00401-0045.pdf | 13:33 |
| F71 | there's a lot of parks in the area, and it's almost summer | 13:33 |
| Moksparagus | in vitro salivary glands | 13:33 |
| F71 | try not to walk into a communal web | 13:33 |
| delinquentme | i want to search for http://pastie.org/3770073 and replace it with an almost as large string | 13:33 |
| delinquentme | i mean search for the contents of that pastie | 13:33 |
| kanzure | whaat i don't understand | 13:34 |
| Moksparagus | n/m, not what I was thinking | 13:34 |
| kanzure | do you have this verify_data in places? | 13:34 |
| kanzure | and you want to replace the code? | 13:34 |
| Moksparagus | they just cultured cells and analyzed the medium afterwards | 13:34 |
| F71 | I've got some gloveboxes if they freak you out | 13:34 |
| kanzure | delinquentme: a few options.. first.. just delete all the others, and import a single file | 13:34 |
| kanzure | delinquentme: or redefine the function in ruby | 13:34 |
| kanzure | so that you don't have to rewrite the other ones. i don't understand why you have more than 1 copy of that function? | 13:34 |
| diginet | well I was thinking of trying to implant the gene into the duckweed, they double their mass every 24hrs, and have up to 45% protein by dry weight | 13:34 |
| delinquentme | kanzure, ok how about i just search for that string and delete it | 13:35 |
| F71 | crap that's awesome | 13:35 |
| delinquentme | that should be easier | 13:35 |
| diginet | indeed | 13:35 |
| diginet | I believe their plastome has been sequenced as well | 13:35 |
| kanzure | delinquentme: like this? sed -i 's/verify_data/old_verify_data/g' *.rb | 13:35 |
| diginet | they also grow on water, so they're hella easy to grow hydroponically | 13:35 |
| F71 | suddenly, buffalo bayou is covered in indestructible duckweed | 13:36 |
| delinquentme | kanzure, sure where verify_data = that entire ~30 lines of code | 13:36 |
| Steel_ | diginet, which gene is this? | 13:36 |
| diginet | MaSpI and MaSpII Major Ampullate Spidroins 1 and 2 | 13:36 |
| diginet | the nice part is that the spidroins are stable up to like 200 F, so you can just heat a stew of the particalized leaves to near boiling and denature most of the other protein | 13:37 |
| Steel_ | hmmm | 13:37 |
| Steel_ | but it wouldn't be in strand form no? | 13:37 |
| diginet | no, you need to make a spinneret for that | 13:37 |
| diginet | (which is a different struggle altogether) | 13:37 |
| kanzure | delinquentme: possibly this.... sed -i 's/^ def verify_data\(.*\)^ end//p' my_file.rb | 13:38 |
| Steel_ | is there a benefit to having the spidroins not in strand form? | 13:38 |
| diginet | that's how they come naturally | 13:38 |
| diginet | their secreted by the gland into a dope and spun out by the spider | 13:38 |
| diginet | *they'[re | 13:38 |
| F71 | put all your spider science papers and artificial spinneret stuff somewhere | 13:38 |
| kanzure | delinquentme: you should probably not use -i for now, without -i sed will show you what the modifications will look like (without editing the file) | 13:38 |
| diginet | okaym that'll take awhile | 13:38 |
| F71 | or on a flash drive or soemthing, this project is badass | 13:38 |
| Steel_ | right, diginet, but I mean is there a manufacturing use for them in that form | 13:39 |
| diginet | F71, thanks :) | 13:39 |
| kanzure | diginet: yeah if you put your files on dropbox i'll upload to the http paper server | 13:39 |
| diginet | okay, got it | 13:39 |
| delinquentme | check | 13:39 |
| diginet | Steel_ sure, you can make them into films or anything you want | 13:39 |
| Steel_ | hmm | 13:39 |
| diginet | I need to read up on transgenic plants | 13:39 |
| delinquentme | kanzure, what do the carats do? | 13:40 |
| delinquentme | ^ | 13:40 |
| diginet | all I know right now is that putting the genes into the chloroplasts gives like 100-fold better yields | 13:40 |
| diginet | however, most of the research is on doing so in the tobacco plant | 13:41 |
| F71 | yeah but can you express these specific genes in a chloroplast? | 13:41 |
| F71 | Like I doubt it's a one-step | 13:41 |
| F71 | Is it even a single protein? | 13:41 |
| diginet | there's not too much PTM | 13:41 |
| diginet | there's two proteins | 13:41 |
| F71 | or does it agglomerate later? | 13:41 |
| F71 | okay | 13:41 |
| diginet | yeah | 13:41 |
| F71 | Do you have a paper on how it's made? | 13:42 |
| diginet | it turns into a fiber when the pH is lowered, and it is exposed to shearing | 13:42 |
| diginet | i.e. squeezing it/extruding it causes it to self assemble | 13:42 |
| diginet | it's actually rather easy to make | 13:42 |
| F71 | Like, there might me some utilities that a chloroplast doesn't have | 13:42 |
| diginet | the hard part is extracting the protein | 13:42 |
| kanzure | delinquentme: in regular expressions, ^ just means "match beginning of line" and $ means "match end of line | 13:42 |
| kanzure | " | 13:42 |
| diginet | no, the chloroplast doesn't make the protein, it works just like nuclear expression, the ribosomes assemble the peptide chain | 13:43 |
| F71 | also, chloroplasts don't have a golgi apparatus | 13:43 |
| diginet | it's just like a plasmid | 13:43 |
| F71 | oh I see | 13:43 |
| diginet | F71, no the gene goes to the same ribosomes that nuclear genes do | 13:43 |
| F71 | I was like WTF, I didn't know chloroplasts had good expression systems, haha | 13:43 |
| diginet | hahaha yeah | 13:43 |
| diginet | it's rather than there are tons of them in every plant cell, and the plant seems to naturally favour genes in the chloroplast, so you get lots of expression | 13:44 |
| diginet | another nice thing is that the gene is confined to the plant, it cant spread via pollen | 13:44 |
| diginet | (so this would be great for GMOs) | 13:44 |
| diginet | okay, let's do the math here, approx. 2500 silk cocoons equals one pound of silk. One coccoon yields almost a kilometer of silk. so, 2500 kilometers. What we need to know is how many meters, say, a mg of silk would yield | 13:48 |
| diginet | oh, another note: I might've said this before, but I have name for the spider silk fiber: serylar how does that sound? | 13:49 |
| kanzure | find the molecular weight of the protein | 13:49 |
| kanzure | and the length | 13:49 |
| diginet | 300 kDa | 13:49 |
| diginet | no idea on length though | 13:49 |
| kanzure | ok. how many angstroms or nm. | 13:49 |
| F71 | take the cross section of the silk and you can figure volumetrically | 13:49 |
| kanzure | the size should be on ncbi somewhere | 13:49 |
| diginet | okay, I'll look | 13:49 |
| F71 | also, another reason to grab local spiders | 13:49 |
| F71 | chemistry and othersuch experiments | 13:50 |
| diginet | indeed | 13:50 |
| F71 | My best scale is a milligram, and I'm too tied up to build a microgram | 13:50 |
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| diginet | there is, supposedly, one company already commercializing duckweed for the production of protein pharmaceuticals | 13:50 |
| F71 | interesting | 13:51 |
| diginet | meaning it is at least possible | 13:52 |
| diginet | I haven't been able to get any data on how much protein tobacco contains by dry weight, as that is apparently the easiest to modify | 13:52 |
| diginet | oh good, there's some articles in the literature on transformation of duckweed | 13:54 |
| delinquentme | diginet, you're coming up with ways to use modified tobacco so that you're both combining the social good of dis-incentivising tobacco growers to adict people but still giving them a reasonable way out by providing some use for their crop? | 14:01 |
| delinquentme | bc that would b e a goo idea | 14:01 |
| kanzure | no he just wants spider silk | 14:02 |
| F71 | he'll take credit for that though | 14:02 |
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| diginet | well heh, there's a lot in the literature on that very subject | 14:05 |
| delinquentme | f me in the a | 14:05 |
| delinquentme | there is seriously no tool for multi-line search and replace | 14:05 |
| diginet | one paper argues that by 2030, Tobacco could be more important as a means of producing pharmaceuticals than smoking tobacco | 14:06 |
| diginet | the question of how to transform the duckweed chloroplasts does remain, however | 14:06 |
| kanzure | delinquentme: i just showed you how to do it | 14:09 |
| kanzure | regular expressions are 100% compatible with multi-line search and replace | 14:09 |
| delinquentme | diginet, id hope so | 14:09 |
| diginet | delinquentme, yeah me too | 14:10 |
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| delinquentme | http://www.telegraph.co.uk/news/features/9193015/Healthy-competition-in-the-NHS-is-a-sick-joke.html | 14:25 |
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| delinquentme | too many ideas damn | 14:25 |
| delinquentme | also richard branson has awesome genes | 14:25 |
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| kanzure | it's true, pulseaudio ain't fun | 14:27 |
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| delinquentme | kanzure, you didnt happen to notice if there is a way to get rendered XML for the elsevier RSS feeeeeeds | 14:43 |
| delinquentme | did u? | 14:43 |
| delinquentme | NM! | 14:43 |
| delinquentme | GOT IT | 14:43 |
| kanzure | rss is xml.. | 14:44 |
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| delinquentme | kanzure, did you hit any max # of request / second on elsevier? | 14:58 |
| delinquentme | i was thinking about threading it .. but the safe thing to do is let it be slow | 14:58 |
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| nmz787 | http://arxiv.org/abs/1204.1719 | 14:59 |
| kanzure | yes they throttle | 14:59 |
| delinquentme | do you know what the max is? | 14:59 |
| kanzure | no | 15:01 |
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| diginet | ugh, elsevier | 15:10 |
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| kanzure | delinquentme: ok we're moving to github | 15:44 |
| delinquentme | too close for guns? | 15:45 |
| kanzure | what | 15:46 |
| kanzure | someone is setting up the private repo for us | 15:46 |
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| delinquentme | cool | 15:47 |
| delinquentme | but um | 15:47 |
| delinquentme | ones ruby | 15:47 |
| delinquentme | ones python | 15:47 |
| kanzure | not a problem | 15:47 |
| kanzure | it'll work | 15:47 |
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| hmatlock | jrayhawk: the server is slow?.. | 15:55 |
| hmatlock | impossible to do anything on it | 15:55 |
| hmatlock | might be my system, but i'm not going to close everything to find out right now | 15:56 |
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| kanzure | hmatlock: seems fine? | 16:02 |
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| hmatlock | i'll take your word, it's odd that everything else seems to be working fine here | 16:06 |
| kanzure | what? http://ipython.org/ipython-doc/stable/parallel/parallel_multiengine.html | 16:06 |
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| jrayhawk | not seeing any obvious problems | 16:13 |
| jrayhawk | load average: 0.25, 0.91, 1.63 huh, never seen it that high before | 16:14 |
| jrayhawk | feel free to text me if it misbehaves again; i would like to catch it in action | 16:14 |
| ybit | it was yakuake | 16:15 |
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| jrayhawk | oh, right, you don't actually have my number. +1 503.869.8658 | 16:17 |
| ybit | jrayhawk: i'm in 150 irc channels.. would that contribute much to that load average? | 16:17 |
| ybit | 160+ something | 16:17 |
| jrayhawk | Maaaaybe if you're logging and aggressively syncing the logs. | 16:18 |
| kanzure | yeah i might be doing that too | 16:20 |
| kanzure | :) | 16:20 |
| kanzure | delinquentme: commit | 16:21 |
| jrayhawk | My quick google searches make no mention of that being an issue, so irssi is probably letting the filesystem/block layers determine transaction grouping. | 16:22 |
| jrayhawk | So yeah, you're probably fine. | 16:22 |
| kanzure | if doc brown was to store a delorean then i think he'd store it in a u-haul storage shed | 16:24 |
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| kanzure | delinquentme: dunno what you're doing | 16:52 |
| kanzure | but if you were grabbing rss | 16:52 |
| kanzure | apparently i did that in 2009 http://diyhpl.us/~bryan/sciencedirect/sciencedirect-rss-urls.txt | 16:52 |
| kanzure | so have fun with that.. | 16:52 |
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| delinquentme | the list seems short no? | 16:53 |
| kanzure | *shrug* this is why verification is important | 16:54 |
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| diginet | oh my ****ing god! look at this: http://www.plantadvanced.com/friday.php | 17:04 |
| delinquentme | diginet, those are nice looking plants | 17:05 |
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| delinquentme | diginet, i see a penis | 17:05 |
| diginet | no, read the page | 17:05 |
| delinquentme | so they modify the plant to make | 17:06 |
| delinquentme | syrums? | 17:06 |
| diginet | no, they modify the plant to express proteins, except it is excreted into the sterile pitcher, basically all you have to do to "purify" it is empty the pitcher | 17:07 |
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| delinquentme | well along with ensuring that theres no crazy particulates in the air | 17:11 |
| delinquentme | and I guess they feed the plant simply by feeding other pitchers? | 17:11 |
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| nmz787 | kanzure: simon says to try using a blu-ray drive to etch PDMS, but he avoided talking about custom optics | 17:15 |
| kanzure | meh blu-ray diode is fun but let's just use a laser | 17:17 |
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| kanzure | pfft "scienceexchange is the airbnb of science" | 17:20 |
| nmz787 | kanzure: he mentioned blu-ray because its got a spot size of less than 1 micron already (405nm laser), and he said it /could/ be better quality spot than $1000s in custom optics (but stayed away from answering my questions about custom stuff, which should amount to about 3 lenses) | 17:22 |
| kanzure | 405nm is the wavelength, not the spot size? | 17:22 |
| nmz787 | right | 17:22 |
| kanzure | are you sure the bluray spots ize is <1 micron? | 17:23 |
| nmz787 | spot size is around 580nm, according to one paper cited by wikipedia bluray page | 17:23 |
| kanzure | *spot size | 17:23 |
| kanzure | hmm | 17:23 |
| kanzure | win 4 | 17:32 |
| kanzure | fdaskfjsakldfa | 17:32 |
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| kanzure | http://syntheticgenomics.com/media/press/020212.html | 17:38 |
| kanzure | idt+sgi partnership | 17:38 |
| kanzure | "Today, IDT synthesizes and ships an average of 36,000 custom oligos per day to more than 86,000 customers worldwide." | 17:38 |
| delinquentme | so whats the subtraction process like kanzure nmz787 | 17:42 |
| delinquentme | are you burning plastic off? | 17:42 |
| delinquentme | PDMS * | 17:42 |
| nmz787 | kanzure: i think someone in here was saying CO2 laser is cleaner signal anyway | 17:42 |
| nmz787 | and i think we can control it with analog | 17:42 |
| nmz787 | instead of PWM... which i think will result in smoother cuts | 17:43 |
| nmz787 | delinquentme: I imagine about 50 microns of PDMS and 50 microns of acrylic | 17:43 |
| nmz787 | the reason so the PDMS is definitely cut through all the way | 17:43 |
| kanzure | it's sometimes melting, burning, or vaporizing | 17:44 |
| delinquentme | nmz787, I dont follow | 17:44 |
| delinquentme | well melting you're going to need to clean it | 17:44 |
| delinquentme | I guess you could put it under some kind of heavy airflow | 17:44 |
| nmz787 | like spread peanut butter on a toast | 17:44 |
| nmz787 | say you want to remove lines of peanut butter | 17:44 |
| delinquentme | adhoc analogies | 17:44 |
| nmz787 | instead of scraping the pb off the toast | 17:45 |
| delinquentme | lol | 17:45 |
| delinquentme | yeahh... | 17:45 |
| nmz787 | you take a little toast with you for good measure | 17:45 |
| nmz787 | sacrificial toast layer | 17:45 |
| delinquentme | lol | 17:45 |
| nmz787 | mmmmmmm | 17:45 |
| delinquentme | but that still doesnt tell me how the PB is coming off | 17:45 |
| nmz787 | ahh | 17:45 |
| nmz787 | burning | 17:45 |
| delinquentme | so vaporizing it | 17:45 |
| nmz787 | prob turning into silanes and CO2 and CO | 17:45 |
| nmz787 | hopefully fully vaping | 17:46 |
| delinquentme | heat distortion in the material? | 17:46 |
| kanzure | "In vaporization cutting the focused beam heats the surface of the material to boiling point and generates a keyhole. The keyhole leads to a sudden increase in absorptivity quickly deepening the hole. As the hole deepens and the material boils, vapor generated erodes the molten walls blowing ejecta out and further enlarging the hole." | 17:46 |
| delinquentme | periodic cooling? | 17:46 |
| kanzure | "Non melting material such as wood, carbon and thermoset plastics are usually cut by this method." | 17:46 |
| delinquentme | whelp | 17:46 |
| nmz787 | I don't think its vaporizing then, its plain burning | 17:46 |
| delinquentme | this is not wewd | 17:46 |
| nmz787 | combusting | 17:46 |
| nmz787 | well he makes a good point, phase change is a thing | 17:47 |
| nmz787 | i'd say wood burns | 17:47 |
| nmz787 | vapor to me means soluble in gas | 17:47 |
| nmz787 | i.e. pahse change occurs | 17:47 |
| kanzure | "The energy delivered by the laser changes the surface of the material under the focal point." | 17:48 |
| kanzure | "It may heat up the surface and subsequently vaporize the material, or perhaps the material may fracture (known as "glass" or "glass up") and flake off the surface. This is how material is removed from the surface to create an engraving." | 17:48 |
| nmz787 | I dont think PDMS will warp from heat | 17:48 |
| nmz787 | its basically glass with some carbon added for stickiness | 17:48 |
| nmz787 | hmm | 17:48 |
| delinquentme | nmz787, then what about material running | 17:49 |
| nmz787 | running? | 17:49 |
| delinquentme | as it heats is becomes less viscous | 17:49 |
| nmz787 | its crosslinked | 17:49 |
| nmz787 | no phase changing for it | 17:49 |
| delinquentme | you're telling me that you're hitting it with sufficient energy to vaporize it | 17:49 |
| nmz787 | hmm | 17:49 |
| delinquentme | but theres no phase change... | 17:49 |
| delinquentme | channels being cut how far apart? | 17:49 |
| nmz787 | well combustion is a phase change, but its also a chemical change | 17:49 |
| nmz787 | it doesnt go back to PDMS on cooling | 17:50 |
| delinquentme | true | 17:50 |
| kanzure | delinquentme: channel separation distance is not decided yet. but it can't be too thin otherwise the channels will blow out.. | 17:50 |
| delinquentme | yeah thats what I was worried about | 17:50 |
| kanzure | 10-25 microns should be good | 17:50 |
| delinquentme | thats a testing thing | 17:50 |
| nmz787 | right | 17:50 |
| nmz787 | we'll have a lot of pieces that test simple devices and ideas | 17:51 |
| delinquentme | i was gonna say stick it in a water bath but the water would diffract the beam | 17:51 |
| nmz787 | like channel wall variation | 17:51 |
| kanzure | hah a channel wall test chip.. alright | 17:51 |
| delinquentme | what if you cooled the shit out of it? | 17:51 |
| kanzure | nmz787: it's probably a function of the swelling of the channel | 17:51 |
| nmz787 | might do something | 17:51 |
| nmz787 | i dunno | 17:51 |
| nmz787 | i think the microness of the CNC will help a lot | 17:52 |
| delinquentme | you could also just periodically submerge it | 17:52 |
| kanzure | why would we need to submerge it? | 17:52 |
| delinquentme | cooling | 17:52 |
| kanzure | why do we need to cool it | 17:52 |
| delinquentme | you want fast prints no? | 17:52 |
| nmz787 | i cut channels before with a desktop laser cutter then viewed them with an interferometer | 17:52 |
| delinquentme | minimize deformation | 17:52 |
| nmz787 | and they looked pretty jagged | 17:52 |
| nmz787 | I don't think it will get that hot | 17:52 |
| nmz787 | we're ablating a very small amount of material | 17:53 |
| delinquentme | another interesting thing would be if there are any advantages / smoothing effect from post cut heating the chip | 17:53 |
| nmz787 | i could be wrong | 17:53 |
| delinquentme | it might smooth down the channels | 17:53 |
| nmz787 | but i think a peltier underneat should be good enough | 17:53 |
| nmz787 | I dont think rebaking will do anything | 17:53 |
| nmz787 | its a thermoset compound | 17:53 |
| delinquentme | ohhh | 17:54 |
| nmz787 | i seem to remember them baking around 60 or 80 C | 17:54 |
| delinquentme | so its manufactured as a chunk then fired to set? | 17:54 |
| delinquentme | fucking materials science u so crazy | 17:54 |
| delinquentme | plastic that thinks its ceramic | 17:54 |
| nmz787 | well it starts as a polymer of PDMS, then you add a crosslinking agent and set it... then you ablating the matrix | 17:57 |
| delinquentme | oh | 17:57 |
| delinquentme | i learned about ablation from MassEffec 2 | 17:57 |
| nmz787 | PDMS without the crosslinking agent is a silicone oil | 17:57 |
| delinquentme | #TrueStory | 17:57 |
| nmz787 | that a movie or game? | 17:57 |
| delinquentme | game | 17:58 |
| nmz787 | ahh | 17:58 |
| d3nd3 | what are your opinions on drug use as enhancing the human experience ? | 18:00 |
| delinquentme | quickest way to clearn "\r\n\t\t Showing 1-100 of 165\r\n\t\t " | 18:00 |
| delinquentme | PHOR IT | 18:00 |
| delinquentme | I mean is your brain usable afterwards | 18:01 |
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| nmz787 | everyone uses drugs | 18:01 |
| d3nd3 | so these drugs should be made legal ? | 18:01 |
| nmz787 | many drugs are legal | 18:01 |
| d3nd3 | the ones which seem to have effects on the minds experience seem to be heavily legalized | 18:02 |
| d3nd3 | this is a bad move by governments?? if its a change in experience which we seek ? | 18:02 |
| kanzure | legalized means "it's legal" | 18:03 |
| delinquentme | I like the part where I lost a chunk of skin but bc proper care | 18:04 |
| delinquentme | I KEPT IT! | 18:04 |
| delinquentme | and its growing back | 18:04 |
| delinquentme | aweshum. | 18:04 |
| d3nd3 | so do you take drugs to have an enhanced experience in life? if so which ones do you recommend | 18:08 |
| nmz787 | the good ones | 18:09 |
| d3nd3 | i am seeking enhanced experience of life and consciousness, how my mind is working, drugs is one way to have a different, perhaps better experience , or more intense, how ever you want to say it... do you know any other alternatives to drugs for a similar effect ? | 18:09 |
| nmz787 | yoga | 18:09 |
| nmz787 | meditation | 18:09 |
| nmz787 | read DMT: the spirit molecule by dr rick strassman | 18:10 |
| nmz787 | says those states could very well be the same as those experienced when deep in meditation | 18:10 |
| nmz787 | cool idea, haven't heard much about this stuff recently | 18:11 |
| d3nd3 | where is dmt leagal do you know, i really do want to experience this wonderful thing, i would not have said i lived until i experienced it | 18:11 |
| nmz787 | not a lot goin on in this country re: fixing psychological or physical medical probs | 18:11 |
| nmz787 | south america | 18:11 |
| nmz787 | there are churches in the U.S. but I don't think you can join the religion | 18:12 |
| nmz787 | http://www.ncbi.nlm.nih.gov/pubmed/15163593 | 18:13 |
| nmz787 | that writer's been at this topic for years | 18:13 |
| nmz787 | but even some of this old-world stuff is exploited in modern pharmacopeia that doctors in hospitals prescribe | 18:14 |
| delinquentme | d3nd3, do you excercise ? | 18:15 |
| delinquentme | when was the last time you were gasping for breath outside of a sex encounter | 18:15 |
| d3nd3 | terrence mckenna he died of brain cancer, he also took much drugs during his life, which put chemicals in the brain and alter his perception of life? ... surely there is connection, surely this is an indication that its perhaps a dangerous route to take drugs on a regular basis it causes damage in the head ? of the cells ? cancerous ? | 18:16 |
| delinquentme | i love the fact that logic is exposed in the URL | 18:17 |
| delinquentme | HAX on the IEEE | 18:17 |
| delinquentme | d3nd3, the issue with many drugs is they're not so thouroughly tested | 18:17 |
| nmz787 | kanzure: http://www.instructables.com/id/Burning-visible-images-onto-CD-Rs-with-data-beta/ | 18:18 |
| nmz787 | good way to test if a blu-ray will etch PDMS? | 18:19 |
| nmz787 | it would be raster, not vector, but maybe a good test of laser power? | 18:19 |
| d3nd3 | Non drug-users die EVERY DAY! | 18:23 |
| d3nd3 | this is someone's point ... | 18:23 |
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| d3nd3 | i am curious to ask what are you talking about above, a project of some sort? what are you trying to achieve with a blu ray ? | 18:27 |
| d3nd3 | Is anyone working on an exciting project? or are you mostly all learning through study/reading? ... The purpose of your existences so far is to gain as much knowledge in this century? to be on top of the board game ready to do the ground-breaking moves towards a changed reality ? | 18:30 |
| d3nd3 | what are your goals, what are you working towards | 18:31 |
| d3nd3 | :P | 18:31 |
| kanzure | no, we're building projects | 18:33 |
| kanzure | spiderman is breeding silk webs, yash is doing human muscle enhancement, nmz787 is building a laser cutter and dna synthesizer | 18:34 |
| kanzure | delinquentme is stealing all known science | 18:34 |
| kanzure | etc. etc. | 18:34 |
| diginet | speaking of me: does anyone have information on suppressing competing proteins in recombitant expression systems? | 18:36 |
| delinquentme | etc etc | 18:36 |
| delinquentme | diginet, what is a recombitant * * | 18:37 |
| kanzure | delinquentme: well there's like 60 people in here, i can't possibly summarize all the projects in one sentence | 18:37 |
| delinquentme | kanzure, ohh you're talking w d3nd3 | 18:37 |
| kanzure | delinquentme: recombinant expression system is just how you get cells to grow proteins for you | 18:37 |
| delinquentme | d3nd3, havnt you been in here before? | 18:37 |
| delinquentme | so to answer diginet wouldn't he be looking for "promoters" ? | 18:38 |
| delinquentme | basically add more introns | 18:38 |
| diginet | no, I know about promoters of course, I'm saying would also suppressing other proteins which aren't essential help things | 18:39 |
| d3nd3 | i am always here | 18:39 |
| d3nd3 | but i know so little... | 18:40 |
| delinquentme | diginet, It might be a good bet to leave it be if you're not sure | 18:41 |
| delinquentme | like we're stupidly complex | 18:41 |
| delinquentme | now there ARE existing metabolic charts which map out connections and influences | 18:41 |
| delinquentme | but! | 18:41 |
| delinquentme | futzing with more stuff means you're dealing with more variables | 18:41 |
| delinquentme | BUT! | 18:41 |
| delinquentme | that might also be untrue | 18:41 |
| diginet | true | 18:41 |
| diginet | I could stunt growth and obviate any gains I might've made in the first place | 18:42 |
| delinquentme | I can tell you that lots of cellular processes depend on the concept of thresholds | 18:42 |
| delinquentme | has anyone researched into getting tits to grow? | 18:42 |
| delinquentme | bc seriously that would be a massive business | 18:42 |
| delinquentme | ( if its not obv already ) | 18:43 |
| d3nd3 | hehe | 18:43 |
| delinquentme | injection > surgery | 18:43 |
| delinquentme | id be really curious to see what the market for implants does | 18:43 |
| diginet | isn't "tits" metonymy? I thought a "tit" was a nipple, not the breast | 18:44 |
| d3nd3 | how would this be possible? the skin would to also get bigger aswell as the organ inside the breast ? | 18:44 |
| diginet | I hate that word, it's so puerile and just immature | 18:44 |
| kanzure | so apparently none of you know how a breast works | 18:45 |
| d3nd3 | =) | 18:45 |
| d3nd3 | it looks like an intestine, yes ? | 18:45 |
| d3nd3 | so its a tubular organ? | 18:45 |
| d3nd3 | size of an organ .. growth of an organ, thats the topic yes ? | 18:46 |
| d3nd3 | has it ever been possible ? | 18:46 |
| * delinquentme keeps the mysogny to himself | 18:48 | |
| delinquentme | umm if you're stimulating fat deposits over time | 18:49 |
| delinquentme | the skin will grow to accommodate | 18:49 |
| delinquentme | OH YOU SILLY ENGINEERS | 18:53 |
| delinquentme | YOUR WEBSITE IS SO CONSISTENT | 18:53 |
| delinquentme | SCRAPEABILITY SCORE OF 10+ | 18:53 |
| delinquentme | BRB! | 19:01 |
| diginet | also, what about protease inhibitors where the genome isn't known? I.e. I can't just suppres the protease gene, are there transgenic anti-proteases? | 19:01 |
| ParahSailin | if there is a specific protease you are trying to inhibit, you can do that | 19:02 |
| diginet | ah okay | 19:02 |
| diginet | I probably don't need to worry about it at this point | 19:03 |
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| diginet | uh oh | 19:07 |
| diginet | I exceeded the maximum download for my library account :( | 19:07 |
| joshcryer | There is a confluence of nicknames that start with 'D' which is fascinating to observe but not engage. | 19:08 |
| diginet | should I be flattered or insulted | 19:09 |
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| joshcryer | Both. | 19:20 |
| delinquentme | Neither. | 19:24 |
| delinquentme | im all fuck yehhh the shits verified! | 19:33 |
| kanzure | nmz787: ping | 19:35 |
| kanzure | http://code.google.com/p/lemoncurry/wiki/main | 19:35 |
| kanzure | "Open Source UV Photopolymer DLP 3D Printer" | 19:35 |
| kanzure | see #lemoncurry | 19:35 |
| joshcryer | Nice. | 19:36 |
| nmz787 | kanzure: email again | 19:36 |
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| kanzure | http://www.youtube.com/watch?v=qKhit2nsoq4 | 19:37 |
| nmz787 | ! oO | 19:37 |
| kanzure | firmware: https://github.com/kliment/Sprinter | 19:37 |
| kanzure | stepper driver: http://www.pololu.com/catalog/product/1183 | 19:37 |
| nmz787 | we should talk to him | 19:38 |
| nmz787 | yeah thats the one fenn was talking abouth | 19:38 |
| nmz787 | well almost, thats the one i think we should get | 19:39 |
| nmz787 | its $7 more but has voltage regulation | 19:39 |
| nmz787 | i'm not sure what resolution this could give us though | 19:40 |
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| nmz787 | each pixel is already 4-40 microns | 19:40 |
| nmz787 | (not sure) | 19:40 |
| nmz787 | in this case it looks like he's enlarging the image too | 19:40 |
| kanzure | i wonder why they are using dlp to do 3d printing | 19:40 |
| joshcryer | diginet, I was just teasing btw. | 19:40 |
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| nmz787 | instead of LCD? | 19:41 |
| kanzure | well i guess they want small features | 19:42 |
| delinquentme | http://www.jstor.org/action/showPublication?journalCode=blackcamera | 19:42 |
| delinquentme | #trueStory | 19:42 |
| delinquentme | #ThisIsWhyRacism | 19:42 |
| kanzure | why the hell are you using twitter hashtags | 19:43 |
| kanzure | this isn't twitter | 19:43 |
| kanzure | blah | 19:43 |
| nmz787 | http://www.youtube.com/watch?v=HL3Wmg7N9V8 | 19:44 |
| nmz787 | so we need to hack a bd-r | 19:44 |
| kanzure | music is awesome | 19:44 |
| nmz787 | newegg $80-$100 | 19:44 |
| nmz787 | says 450mW | 19:44 |
| kanzure | electrical tape at 9 ft.. hahah | 19:45 |
| nmz787 | ya | 19:45 |
| nmz787 | if he can do that, maybe PDMS is no prob | 19:45 |
| kanzure | looks like he's carving wood no problem | 19:45 |
| kanzure | hmm | 19:48 |
| kanzure | well that significantly cuts down on the price | 19:48 |
| diginet | haha, is anyone here interested in gene guns/biolistics? because it looks like I'm going to have to be building one | 19:49 |
| kanzure | i think you should use an electroporator instead | 19:50 |
| kanzure | thomasegi was designing one with yashgaroth | 19:50 |
| audy | kanzure for tissue? | 19:50 |
| kanzure | iirc it was like a $30 circuit | 19:50 |
| kanzure | oh right. well anyway, it's a very similar circuit either way | 19:50 |
| audy | you can gene gun DNA into a plant or your little brother | 19:50 |
| audy | very difficult to electroporate your little brother much less make him competent | 19:51 |
| diginet | mentally, or biologically for that matter | 19:51 |
| delinquentme | ZOMG GENE GUN | 19:51 |
| delinquentme | BAZOOM | 19:51 |
| delinquentme | Also. | 19:51 |
| audy | I might be as simple as modifying a shotgun shell | 19:51 |
| delinquentme | Kara No Kyoukai | 19:51 |
| diginet | I dunno about that | 19:51 |
| diginet | maybe though | 19:51 |
| delinquentme | audy, you're qualified | 19:52 |
| nmz787 | http://www.rolanddga.com/products/milling/imodela/ | 19:52 |
| nmz787 | Software resolution0.00039 inches/step (0.01 mm/step; RML-1),0.000039 inches/step (0.001 mm/step: NC code)Mechanical resolution0.00000732 inches/step (0.000186 mm/step: micro steps) | 19:52 |
| kanzure | delinquentme: commit the scrapers | 19:52 |
| diginet | lol, gene guns | 19:52 |
| delinquentme | the barnacles are too hard kanzure | 19:52 |
| audy | *BOOM* now you're expressing GFP in your butt | 19:52 |
| kanzure | delinquentme: what | 19:52 |
| diginet | YES | 19:52 |
| kanzure | delinquentme: if you commit the files, i will do the search-and-replace thing | 19:52 |
| delinquentme | lol i already did that | 19:53 |
| diginet | electroporation is hard for plant cells, and not very useful for chloroplast transformation | 19:53 |
| audy | eeek yeah how do you get DNA into the choloroplasts? | 19:53 |
| kanzure | diginet: for plants you should just use agrobacterium | 19:54 |
| diginet | basically, luck | 19:54 |
| audy | culture them separately? | 19:54 |
| diginet | kanzure, useless for chloroplasts | 19:54 |
| kanzure | ParahSailin: do you have any agrobacterium experience | 19:54 |
| audy | you do triparental mating | 19:54 |
| ParahSailin | i have a friend in lubbock who is working with them and can get me bugs and plasmids | 19:54 |
| diginet | audy, basically you use a cell type which has fewer chloroplasts, insert a marker, and select for it very aggressively until you get a homoplastid cell | 19:55 |
| diginet | kanzure, ParahSailin, actually that may be useful, as I need two different strains of the target, one for each protein and was thinking of using GFP as a visual marker to differentiate each | 19:56 |
| diginet | but that will be later on | 19:56 |
| ParahSailin | this friend is actually the one who told me about this channel but i dont know what name he goes under here | 19:56 |
| diginet | audy, if you're interested I can upload a few papers I have on plastid transformation | 19:57 |
| audy | diginet I got google scholar thanks :) | 19:57 |
| diginet | no problem | 19:57 |
| kanzure | diginet: i'm still waiting on your paper dump :) | 19:58 |
| diginet | kanzure, I know I know, I have so many folders to go through, it's gonna take awhile | 19:58 |
| diginet | I have to take beaks every now and then | 19:58 |
| diginet | *breaks | 19:58 |
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| * diginet is confused | 20:02 | |
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| ybit | diginet: on occassion i change my name to the word i'm unintentionally highlighted with, if that's what your confusion is about | 20:09 |
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| diginet | haha | 20:12 |
| delinquentme | LOLOL | 20:13 |
| delinquentme | how long does google keep emails? | 20:26 |
| kanzure | forever | 20:26 |
| delinquentme | nah | 20:27 |
| delinquentme | unless i cant search forever back | 20:27 |
| delinquentme | PS why does gmail search suck balls? | 20:27 |
| delinquentme | im looking for a password I saved and I specifically recall a portion of the term | 20:27 |
| kanzure | gmail search takes 30-45sec each time for me | 20:28 |
| delinquentme | but gmail search for some reason only searches whole words | 20:28 |
| kanzure | granted, it has hundreds of thousands of emails to search | 20:28 |
| delinquentme | archives are searched by default right? | 20:28 |
| delinquentme | does it search reliably for you? | 20:28 |
| delinquentme | bc mine is shitty | 20:28 |
| nmz787 | i periodically zombify and go through old emails, its boring but it doesn get rid of some clutter | 20:28 |
| kanzure | nope it takes forever, so if i typo i have to wait another minute or two | 20:28 |
| delinquentme | kanzure, u got a new email in ur inbox | 20:32 |
| kanzure | hmm | 20:32 |
| kanzure | loading | 20:32 |
| kanzure | was this sent to you? | 20:33 |
| kanzure | or did you send this | 20:33 |
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| delinquentme | anyone know of a quick way to break into a dlink router without having to reset it? | 20:36 |
| diginet | default password? | 20:36 |
| delinquentme | yeah i changed it | 20:38 |
| delinquentme | OK 1 more then break time | 20:45 |
| delinquentme | ["Asian Journal of Mathematics", "http://www.intlpress.com/AJM/"] | 20:53 |
| delinquentme | you know thats a good ass math journal | 20:53 |
| _Sketch_ | Heh. | 20:53 |
| _Sketch_ | ass-math. | 20:53 |
| delinquentme | _Sketch_, dont be racist. | 20:53 |
| _Sketch_ | http://xkcd.com/37/ | 20:53 |
| ParahSailin | difficulty level asian | 20:55 |
| delinquentme | <adjective>-ass <noun> better? | 20:55 |
| nmz787 | kanzure: http://www.repairfaq.org/sam/laserhtr.htm#htrichl | 21:00 |
| kanzure | sam's laser faq is the best | 21:01 |
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| nmz787 | kanzure: "CO2 lasers are generally used for cutting materials like stainless steel because they can, in general, be focused to smaller spots, which improves cut quality. You can focus a 1 kW CO2 laser to a 100 micron spot. A 1 kW YAG is generally used with fiber optics for beam delivery and can't be focused smaller than 400 microns or so." | 21:03 |
| kanzure | hrm | 21:03 |
| kanzure | yep! that's the solution, we'll cut our chips out of stainless steel. haha | 21:06 |
| kanzure | and then arc weld the two layers together | 21:06 |
| nmz787 | too bad we can't do 3D channels with SLS or that yellow curry thing | 21:08 |
| nmz787 | but fill the last cut layer with like, wax or something | 21:08 |
| nmz787 | so we can just keep adding layers | 21:08 |
| nmz787 | then melt the wax out at the end | 21:08 |
| nmz787 | :/ | 21:09 |
| kanzure | you could probably boil the wax or acid etch it out | 21:09 |
| nmz787 | then it would be more automated, no probs bonding layers | 21:10 |
| kanzure | we should probably just design for a bluray diode | 21:12 |
| kanzure | but also design the structure to accomodate a giant laser | 21:12 |
| kanzure | and a co2 tube | 21:12 |
| kanzure | and those crazy additional optics | 21:12 |
| * delinquentme found a bug in ruby | 21:13 | |
| kanzure | commit yer code | 21:14 |
| nmz787 | what if bluray takes too long | 21:16 |
| nmz787 | hrmm | 21:16 |
| delinquentme | i thought | 21:20 |
| delinquentme | '/asdf/'.split('/').count == 2 | 21:20 |
| delinquentme | shouldn't it be 3? | 21:20 |
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| kanzure | delinquentme: no because there's no content after the last one | 21:26 |
| Moksparagus | does anybody know why embedded device manufacturers obfuscate the kernel/drivers of their devices? | 21:26 |
| delinquentme | kanzure, theres no content for the first one! | 21:27 |
| * delinquentme mindplodes | 21:28 | |
| kanzure | >> '/a/b/c/d/e'.split('/') | 21:28 |
| kanzure | => ["", "a", "b", "c", "d", "e"] | 21:28 |
| kanzure | try lsplit and rsplit | 21:28 |
| kanzure | oh ruby doesn't have that | 21:29 |
| kanzure | well. python does. | 21:29 |
| kanzure | nmz787: check this out.. | 21:30 |
| kanzure | my grandma emailed me this a few months ago | 21:30 |
| kanzure | "Steve Jurvetson, a director of Synthetic Genomics, is part of a group of very rich, very bright Singularity observers who end up somewhere in the middle on the philosophy’s merits — optimistic about the growing powers of technology but pessimistic about humankind’s ability to reach a point where those forces can actually be harnessed." | 21:30 |
| kanzure | "Mr. Jurvetson, a venture capitalist and managing director of the firm Draper Fisher Jurvetson, says the advances of companies like Synthetic Genomics give him confidence that we will witness great progress in areas like biofuels and vaccines." | 21:30 |
| kanzure | "Still, he fears that such technology could also be used maliciously — and he has a pantry filled with products like Spam and honey in case his family has to hunker down during a viral outbreak or attack." | 21:30 |
| kanzure | '“Thank God we have a swimming pool,” he says, noting that it gives him a large store of potentially potable water.' | 21:30 |
| kanzure | i think that's hilarious | 21:31 |
| delinquentme | kanzure, '/asdf/'.split('/',-1) | 21:31 |
| delinquentme | extensible and consistend | 21:31 |
| delinquentme | unlike me spulling | 21:32 |
| kanzure | "Also, in the interest of making sure the record is straight, I do believe that Steve is not the only person who originated the idea of adding artificial chromosome add-ons to the human genome. I had the inklings of the idea in the mid-1990's [2]." | 21:32 |
| kanzure | "I founded a company, Robiobotics, circa 2001 to pursue that objective but its life was cut short by the dot-com bust. The idea was however presented to Steve Jurvetson at DFJ and I believe it subsequently led to the funding of Synthetic Genomics [3]." | 21:32 |
| delinquentme | is it evil to threaten to hack girls FBs | 21:33 |
| delinquentme | lololol | 21:33 |
| kanzure | delinquentme: as long as you don't leave a baby on the profile | 21:33 |
| delinquentme | LOL! | 21:33 |
| kanzure | aww shit i have his email from 2007 | 21:34 |
| kanzure | Steve Jurvetson <steve@dfj.com> | 21:34 |
| kanzure | also this guy: sj@dfj.com | 21:35 |
| kanzure | Timothy Draper <tim@dfj.com> | 21:35 |
| kanzure | Warren Packard <warren@dfj.com> | 21:35 |
| kanzure | "Space Investment Summit 5... The Keynote Speaker will be Steve Jurvetson, Managing Director at Draper Fisher Jurvetson, and founding VC investor in Hotmail." | 21:36 |
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| delinquentme | warren packard | 21:38 |
| delinquentme | thats an astronaut name | 21:38 |
| kanzure | http://www.dfj.com/team/teamdetail.php?10155 | 21:38 |
| kanzure | eww "Intellectual Ventures" | 21:38 |
| delinquentme | so is shepard | 21:38 |
| delinquentme | real time sports discovery | 21:39 |
| delinquentme | oh | 21:39 |
| delinquentme | wow | 21:39 |
| delinquentme | yip | 21:39 |
| delinquentme | ee | 21:39 |
| kanzure | http://www.techstars.org/mentors/bbryant/ i think this guy is also from dfj | 21:40 |
| nmz787 | kanzure: | 21:42 |
| nmz787 | http://onlinelibrary.wiley.com/doi/10.1002/lapl.200710123/abstract | 21:42 |
| kanzure | neat | 21:42 |
| nmz787 | that says for soft tissue they used 1735 W/cm sq | 21:42 |
| nmz787 | i have calculated (which Simon didn't completely straightforward say was correct) 27 million W/cm sq | 21:43 |
| nmz787 | for 1W @ 6 micron spot | 21:43 |
| nmz787 | that sounds like a hell of a lot | 21:44 |
| kanzure | sounds like we're going to explode our pdms :) | 21:44 |
| delinquentme | http://www.iospress.nl/journal/ai-communications/ | 21:44 |
| delinquentme | Impact factor of ... 0.837 | 21:44 |
| delinquentme | do you even advertise that? | 21:44 |
| kanzure | haha | 21:45 |
| kanzure | "Impact factor of zero, yo" | 21:45 |
| delinquentme | "were underground" | 21:45 |
| kanzure | hipster impact factor | 21:46 |
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| nmz787 | kanzure: BH12LS38 | 21:56 |
| nmz787 | whoops | 21:56 |
| nmz787 | http://www.ebay.com/itm/LG-Internal-Blu-ray-Drive-BH12LS38-12x-BD-DVD-Rewriter-Super-Multi-Blue-/160782110321?pt=PCC_Drives_Storage_Internal&hash=item256f5c4e71#ht_500wt_1287 | 21:56 |
| Guest73883 | lemon curry dlp? | 21:56 |
| * kanzure nods | 21:56 | |
| Guest73883 | why use a blue ray drive instead of a regular dlp? | 21:57 |
| kanzure | this is just for our laser cutter | 21:57 |
| Guest73883 | ohhhh | 21:58 |
| Guest73883 | will that be the actual laser diode for it? | 21:59 |
| kanzure | probably, still deciding.. | 22:00 |
| kanzure | a co2 laser would give us more options | 22:00 |
| kanzure | but a bluray diode would be super cheap | 22:01 |
| Guest73883 | how much power does bluray provide? | 22:01 |
| nmz787 | not just cheaper, but easier to make small | 22:01 |
| nmz787 | 700mW | 22:01 |
| nmz787 | supposedly focused to <1micron | 22:01 |
| nmz787 | i guess they spin the hell outta those discs to keep them from burning | 22:02 |
| nmz787 | ".or, knowing that the track length for CDs is 5,378m, the track length for DVDs is roughly: | 22:03 |
| nmz787 | 5,378 * CD Track Pitch / DVD Track Pitch = 5,378 * 1.60/0.74 metres | 22:03 |
| nmz787 | and the track length for BDs is: | 22:03 |
| nmz787 | 5,378 * CD Track Pitch / BD Track Pitch = 5,378 * 1.60/0.32 metres" | 22:03 |
| nmz787 | and we have 31 meters to cut | 22:04 |
| nmz787 | (bd has 26890 meters of writable track) | 22:04 |
| nmz787 | "The 12x BD burner that delivers a perfect 25GB disk in under | 22:06 |
| nmz787 | 12 minutes" | 22:06 |
| nmz787 | so i think with that ratio of their lengths, and since the power is constant... i think a bluray drive might actually work nicely | 22:06 |
| delinquentme | kanzure, xpath search for a href value | 22:12 |
| delinquentme | .search('/a[contains(@href,"http://")]') | 22:13 |
| delinquentme | not // bc thats the whole document and I'm pretty sure href is the right selector | 22:13 |
| kanzure | eh | 22:14 |
| kanzure | //a[@href!=''] | 22:14 |
| delinquentme | thats just not empty right | 22:14 |
| kanzure | yes | 22:14 |
| delinquentme | yeah I need to find the one with http :D | 22:14 |
| kanzure | just look at all of them and check for http in ruby | 22:15 |
| kanzure | mylink.include? "http" | 22:15 |
| delinquentme | thatll do | 22:16 |
| kanzure | http://www.gizmag.com/magnifi-iphone-adapter-microscope/22116/ | 22:20 |
| kanzure | http://www.kickstarter.com/projects/637232010/magnifi-the-worlds-first-iphone-photoadapter-case | 22:20 |
| kanzure | http://www.arcturuslabs.com/ | 22:20 |
| kanzure | iphone/microscope fixture. nothing to see here, carry on.. | 22:20 |
| delinquentme | that is pretty fucking sexy | 22:23 |
| delinquentme | wah wah wee wah | 22:23 |
| nmz787 | pretty cool | 22:24 |
| delinquentme | it doesnt show how the clasp works for different size lenses! | 22:27 |
| delinquentme | LIESSSSSSSSSSSSSs | 22:27 |
| kanzure | man, i was totally going to write some pokemon code tonight | 22:28 |
| delinquentme | for integration w google maps NES? | 22:28 |
| kanzure | delinquentme: nope. i've been writing source code to pokemon red | 22:28 |
| kanzure | http://bitbucket.org/kanzure/pokered | 22:29 |
| kanzure | http://bitbucket.org/kanzure/pokecrystal | 22:29 |
| nmz787 | you just need to buy me a big kit 'o lenses to play with | 22:31 |
| nmz787 | and some lasers too | 22:31 |
| nmz787 | gotta have lasers | 22:31 |
| nmz787 | more lasers | 22:31 |
| nmz787 | is teh pokemon kid still Ash? | 22:32 |
| delinquentme | pew pew | 22:32 |
| kanzure | nmz787: in the game? | 22:32 |
| nmz787 | ya | 22:32 |
| delinquentme | I think i have a celeb crush on sarah underwood | 22:34 |
| kanzure | nmz787: nope it's choose-your-own-name or something | 22:36 |
| delinquentme | japan society of applied physics | 22:41 |
| delinquentme | something was lost in translation | 22:41 |
| kanzure | its real name is "japan society of applied giant robots" | 22:47 |
| delinquentme | http://annex.jsap.or.jp/OSJ/opticalreview/ | 22:48 |
| delinquentme | OH man. | 22:48 |
| kanzure | hey man don't hate on SCIENCE and 1995-style HTML | 22:51 |
| kanzure | hah | 22:51 |
| delinquentme | I think a phillipino girl is trying to get me to import her | 22:55 |
| delinquentme | =/ shes like 45 | 22:55 |
| ybit | delinquentme: just one? | 22:55 |
| ybit | come on, you can do better than this | 22:56 |
| delinquentme | lol | 22:57 |
| delinquentme | well at their standard of living i could prob import a whole family | 22:57 |
| delinquentme | teach them to program u know | 22:57 |
| delinquentme | DAMN | 22:57 |
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| delinquentme | am i missing something here | 23:10 |
| delinquentme | " Applied Phsyics Express (APEX)".include? " Applied Physics Express" | 23:10 |
| delinquentme | FALSE? | 23:10 |
| kanzure | it says Phsyics | 23:10 |
| kanzure | not Physics | 23:10 |
| delinquentme | damn. | 23:11 |
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| delinquentme | http://i.imgur.com/ZH7qN.jpg | 23:25 |
| delinquentme | im out | 23:25 |
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| jrayhawk | why is the intake partially dismantled | 23:31 |
| -!- amphetamine is now known as AdrianG | 23:31 | |
| jrayhawk | i guess tubes are just too boring or something | 23:32 |
| katsmeow-afk | maybe they got the intercoolers, which are somewhere else | 23:34 |
| katsmeow-afk | got = gotot | 23:34 |
| katsmeow-afk | goto | 23:34 |
| jrayhawk | I guess that makes sense | 23:34 |
| jrayhawk | http://www.rx7.org/Robinette/images/hoses.gif tubes rule | 23:34 |
| yashgaroth | I just realized I have no idea where the intake even is | 23:35 |
| jrayhawk | it's INSIDE YOUR HOUSE | 23:35 |
| yashgaroth | oh nooooo | 23:35 |
| jrayhawk | RUN | 23:35 |
| yashgaroth | it's...taken me in! | 23:35 |
| diginet | LOUD NOISES | 23:35 |
| katsmeow-afk | BRIGHT FLASHES AND LASER SOUNDS | 23:36 |
| yashgaroth | don't worry guys I beat it away with the alternator or something | 23:36 |
| jrayhawk | http://image.fourwheeler.com/f/9046046+w750+st0/129_0710_10_z+gm_4l60e_transmission_fixes+valve_body.jpg also fluidics rule | 23:37 |
| jrayhawk | it's sorta like tubes, only even more terrifying | 23:38 |
| katsmeow-afk | you'll find plates like that in most automatics | 23:38 |
| yashgaroth | is that to keep the engine entertained on long drives? | 23:38 |
| katsmeow-afk | speaking of fluidics, why wold a gear pump be labeled right hand or left hand? | 23:39 |
| jrayhawk | maybe it's engineered to be more efficient in one direction | 23:39 |
| katsmeow-afk | it should be symetric | 23:39 |
| jrayhawk | I imagine you can do neat tricks with flow dynamics to that end, but I'm not much of an engineer. | 23:40 |
| jrayhawk | man, that last image makes me actually miss the cleansing feel of ATF | 23:43 |
| jrayhawk | i should rectify this by removing my power steering. fucking power steering. | 23:43 |
| jrayhawk | ##carplusroadmap | 23:45 |
| katsmeow-afk | nice thing about not having serpentine belts: when my ps pump sounded like a wood chipper, i dropped the belt and kept driving | 23:46 |
| diginet | you know, since it's going to take awhile to farm enough protein, I should resurect DIY diamonds in the interim | 23:46 |
| diginet | the question is how to go about building the CVD reactor | 23:46 |
| yashgaroth | why don't you ask the guys who made them oh wait they're in a shallow grave somewhere | 23:47 |
| diginet | SERIOUSLY | 23:47 |
| diginet | that's the one conspiracy I believe in, DeBeers is everywhere | 23:47 |
| katsmeow-afk | that sounds severe | 23:47 |
| yashgaroth | yeah well | 23:48 |
| jrayhawk | the killings aren't really going on anymore | 23:48 |
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| yashgaroth | then they had the intended effect | 23:48 |
| katsmeow-afk | ran out of people? | 23:48 |
| diginet | jrayhawk, how comforting :P | 23:49 |
| yashgaroth | people willing to mess with an industry that supplies a product that hitmen like to be paid in | 23:49 |
| jrayhawk | they soooortof delayed the first artificial diamond industry, though obviously the second world had them during that time | 23:49 |
| katsmeow-afk | inherent downside | 23:49 |
| jrayhawk | s/the first/the first world/ | 23:49 |
| yashgaroth | yeah they did alright until the mafia took over running the ussr | 23:50 |
| diginet | the Soviets didn't take their shit, then when the USSR collapsed, they just bought up their cache | 23:51 |
| jrayhawk | actually the USSR was selling diamonds to Oppenheimer the whole time; that's a big part of how they got foreign currency for their espionage efforts. | 23:51 |
| katsmeow-afk | so basically, make the lab portable, and move around, sell infrequently, randomly, different laces, small qtys | 23:51 |
| yashgaroth | hard to make it portable when it's a 20-ton hunk of metal | 23:52 |
| diginet | not interested in selling | 23:52 |
| diginet | I don't care about the money | 23:52 |
| * katsmeow-afk would look annoyed at her nose if she could,, it had a runny cold on the left last nite, and today the cold is on the right | 23:52 | |
| katsmeow-afk | 20 tons 1) floats 2) fits in a shipping container | 23:53 |
| ParahSailin | rothschild runs de beers | 23:55 |
| jrayhawk | what? | 23:55 |
| --- Log closed Thu Apr 12 00:00:32 2012 | ||
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