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| kanzure | beep. | 05:50 |
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| streety | nmz787: thanks for the pointer, I've been considering starting a project recently that the esp8266 looked good for | 07:11 |
| archels | nmz787: are you driving Peltiers or motors? | 07:24 |
| archels | in case of the former I can give you an efficiency report in a few weeks | 07:24 |
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| kanzure | .title http://www.bionet.ee.columbia.edu/projects/neurokernel | 08:22 |
| yoleaux | Neurokernel: Emulating the Fruit Fly Brain | Bionet | 08:22 |
| kanzure | (same as the last time neurokernel was mentioned in here) | 08:22 |
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| chris_99 | noones ever seen anything about a homemade mass spectrometer out of interest? | 10:14 |
| streety | chris_99: seen a few papers on minimal mass spec but nothing homemade as such | 10:23 |
| streety | most of the ones I've seen weren't viable for proteomics so I didn't pay them too much attention | 10:23 |
| chris_99 | ah, cheers for the heads up, yeah i haven't seen anything from a little googling either | 10:24 |
| streety | it would be extremely nice | 10:25 |
| streety | thinking of working on one? | 10:25 |
| chris_99 | well i saw a 2nd hand one that would have been cool, but i don't have the space for one atm alas | 10:26 |
| streety | they are big beasts | 10:26 |
| chris_99 | mmm | 10:26 |
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| chris_99 | check this one! - http://www.purdue.edu/uns/x/2009a/090121CooksMelamine.html | 10:30 |
| streety | http://pubs.rsc.org/en/content/articlelanding/2010/an/b923427f#!divAbstract is their publication | 10:35 |
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| chris_99 | cheers lets see if paperbot can get it | 10:36 |
| chris_99 | paperbot: http://pubs.rsc.org/en/content/articlelanding/2010/an/b923427f#!divAbstract | 10:36 |
| paperbot | http://diyhpl.us/~bryan/papers2/paperbot/Direct%20analysis%20of%20melamine%20in%20complex%20matrices%20using%20a%20handheld%20mass%20spectrometer.pdf | 10:36 |
| chris_99 | yay well done paperbot | 10:37 |
| streety | I didn't realize paperbot was working again | 10:37 |
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| nmz787 | archels: got some motors and peltiers... have had some projects in mind but finding the right project to start on and finding the right drivers has been something I've been working on for a while | 10:45 |
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| nmz787 | chris_99: i've /thought/ of what a home-made mass-spec might be... but the ion/electron optics are a huge undertaking I'm pretty sure. SEMs from like 30-50 years ago couldn't get much past ~3500X magnification from what I've heard... so if the column was applied to a mass-spec I think that measn the mass-to-charge ratio would have limited resolution. That said, I was thinking a SEM or FIB column might be applicable to a hacked together ... | 10:48 |
| nmz787 | ... instrument. | 10:48 |
| nmz787 | not sure if the quadrapol/octopole would be easier to make on a MEMS scale... I've known there have been efforts for MEMS mass-spec for a few years, not sure how long those efforts have been underway in general though. | 10:49 |
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| chris_99 | that sounds interesting | 10:53 |
| chris_99 | theres more variants than i thought | 10:54 |
| nmz787 | the mass-spec is one thing... the way to get the sample in a gas-phase is another (someone mentioned proteomics, which needs a much more advanced 'atomizer'/'gasifier' (not sure the general term for this piece)) | 10:57 |
| chris_99 | could you use ultrasound to create a vapour or is that not good enough for some reason | 10:58 |
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| thundara | chris_99: Most modern mass spec instruments also nee a fair bit of electrical power / pumps to maintain the vacuum, along with liquid nitrogen to keep things cool | 11:02 |
| thundara | Would be pretty hard to do cheaply in a diy setting | 11:02 |
| nmz787 | yeah they sell foggers for frogs and stuff at pet stores | 11:02 |
| nmz787 | thundara: newish FIB/SEM can run on 110V 15A circuits | 11:02 |
| chris_99 | i didn't realise they needed liquid nitrogen | 11:02 |
| nmz787 | I don't remember the mass-spec I used recently needing any LN2 | 11:03 |
| thundara | This is for orbitrap ones | 11:03 |
| thundara | Depends on what you need it for though, proteomics would be pretty hard to do cheaply | 11:04 |
| thundara | But if it's just testing 1 sample's mass, easier | 11:05 |
| thundara | A lot of the HT proteomics stuff works with MS^2 though, which needs a gas (He / N) for ion fragmentation | 11:06 |
| thundara | There's also ECD (electron bombardment), but I'm told that's even more expensive (in terms of instrument cost) | 11:06 |
| thundara | I've hear word of a microfluidics device for measuring molecules' mass/charge, but I think it was more a proof-of-concept than as a fully-featured device with high sensitivity / speed / MS^n | 11:08 |
| thundara | Oh, you linked that above >_< | 11:11 |
| streety | when I've done mass spec proteomics in the past it was MALDI or electrospray ionisation | 11:29 |
| streety | The apparatus didn't seem that complex | 11:29 |
| streety | FT-ICR instruments need liquid nitrogen and helium - orbitraps seem to work similarly so it would make sense they also need cryogenic cooling | 11:33 |
| streety | useful for discovery but if you know what you are looking for simpler instruments should be viable | 11:33 |
| thundara | That's only part of the method though, you have sample prep -> chromatography -> ionization (ESI/MALDI) -> filtering -> fragmentation -> measurement | 11:34 |
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| thundara | Yeah, if you have a simple sample or know what you're looking for, it's easier | 11:35 |
| delinquentme | HOY. | 11:35 |
| thundara | But would be hard to replicate a full proteomics setup in a diy setting | 11:36 |
| thundara | Or at least, you'd want a different approach (Quadrupoles / TOF removes the need for cooling, iirc, unsure what is cheap / good for fragmentation though) | 11:37 |
| t12 | orbitraps dont need cooling | 11:38 |
| streety | the chromatography would be the most challenging part prior to the MS | 11:38 |
| thundara | t12: Erm, yeah, you're right, N gas is for the collision gas and some other parts | 11:40 |
| t12 | fancy mass specs are | 11:40 |
| thundara | http://planetorbitrap.com/download.php?filename=4fb4392c31382.pdf | 11:40 |
| delinquentme | TIL orbitrap | 11:40 |
| t12 | pretty painful to keep going | 11:40 |
| delinquentme | UGH. I love this channel | 11:40 |
| t12 | easier to deal with a core facility | 11:40 |
| thundara | Yep | 11:41 |
| t12 | acqual quadrapoles arent TOO complicated | 11:41 |
| t12 | but way untrivial | 11:41 |
| t12 | easiest diy bet is to frakenstein up various junked mass specs | 11:42 |
| thundara | I'm told the strict tolerances of the machine make it expensive, since having magnetic fields out of alignment gives you incorrect measurements / ions banging into walls | 11:42 |
| t12 | well you calibrate all that out | 11:42 |
| t12 | at some point mechanical becomes limiting i believe | 11:42 |
| t12 | i have a scrap quadrapole around here somewhere | 11:43 |
| thundara | Only within some tolerance, no? Shielding / vacuum are issues too | 11:43 |
| thundara | But maybe this is all propaganda to convince me that mass specs should cost six-seven figures >_> | 11:44 |
| delinquentme | t12 thundara where are you guys geographically | 11:44 |
| delinquentme | ? | 11:44 |
| t12 | https://www.dropbox.com/sc/28n3xpwjdvg93hb/AADuxPlutvSv2sLsG0_rj-r0a | 11:44 |
| t12 | theres a triple quad | 11:44 |
| t12 | in a bathtub | 11:44 |
| t12 | sf | 11:45 |
| t12 | mass specs are pretty impressively built | 11:45 |
| t12 | no single part is really THAT expensive | 11:46 |
| t12 | except maybe an orbitrap itself | 11:46 |
| t12 | or the magnet in an ft | 11:46 |
| t12 | but theres just lots of stuff | 11:46 |
| kanzure | have you seen nmz787's spectrometer design? | 11:46 |
| t12 | neg | 11:47 |
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| thundara | Yeah, the core device is mostly machined metal and electronics | 11:48 |
| thundara | Neg^^ | 11:48 |
| t12 | i have a junk triple quad i need to put somewhere | 11:48 |
| t12 | it might work | 11:48 |
| t12 | needs turbo/controller rework | 11:49 |
| t12 | been offline at at atmosphere for a few years though | 11:49 |
| thundara | Lab has an old one lying around that I was planning to dissect over the summer | 11:49 |
| streety | do you have a link kanzure? | 11:49 |
| kanzure | https://github.com/nmz787/open-spectrometer | 11:50 |
| streety | thanks | 11:50 |
| delinquentme | t12 you're in sf? how don't I know you irl? | 11:50 |
| t12 | unknown | 11:50 |
| delinquentme | core speciality? | 11:50 |
| t12 | god i dunno at this point | 11:51 |
| delinquentme | << servers, web bits of rbotics, python talking shit | 11:51 |
| t12 | previously hpc stuff | 11:51 |
| delinquentme | hentai | 11:51 |
| t12 | right now i do microscope/lab automation | 11:51 |
| t12 | some engineering | 11:51 |
| streety | ah, that's a spectrophotometer, not a mass spectrometer | 11:51 |
| t12 | some machining | 11:51 |
| delinquentme | t12 can I ask for who? | 11:51 |
| t12 | a little electronics/reverseengineering | 11:51 |
| thundara | Automation at a company? or academic | 11:51 |
| t12 | company | 11:51 |
| delinquentme | im currently working in battery / energy grid space ... automation + bidding | 11:52 |
| t12 | holding back name for now | 11:52 |
| t12 | ahh like | 11:52 |
| delinquentme | t12 you wanna come hangout? | 11:52 |
| t12 | that weird ca energy storage mandate thing | 11:52 |
| delinquentme | smoke some crack ? | 11:52 |
| t12 | lol | 11:52 |
| delinquentme | chill out ? | 11:52 |
| delinquentme | cool! | 11:52 |
| delinquentme | t12 if you do the FB thing | 11:52 |
| t12 | ya sure sometime | 11:52 |
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| delinquentme | hmm im not on native irc client ... | 11:53 |
| delinquentme | https://www.facebook.com/delinquentme | 11:53 |
| delinquentme | there we go | 11:53 |
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| t12 | https://www.youtube.com/watch?v=0_x73hpl6io&t=25 | 11:53 |
| delinquentme | yeah add me up if you want. | 11:54 |
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| kanzure | .title | 11:55 |
| yoleaux | Wolf of Wall St - Smoking Crack - YouTube | 11:55 |
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| thundara | t12: I'm curious if you could ECD cheaply for the fragmentation step | 11:56 |
| thundara | Since it's expensive on for-sale instruments, but could just be a patent thing | 11:56 |
| thundara | Unsure about upkeep / eletricity costs for ECD | 11:57 |
| t12 | i've seen installs of some custom ones | 11:57 |
| t12 | i dont think its that bad, its more a matter of having the rest of the stuff to install it in | 11:58 |
| delinquentme | ha! can I like things over IRC? not yet hahaha | 11:58 |
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| thundara | Ah, yeah, I see "electrons" and think "those are cheap!", but I don't really know what actually goes into the method | 11:59 |
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| nmz787 | haha, quadrapole in a bathtub | 12:53 |
| * nmz787 imagines the white-rabbit scene of fear and loathing except with a quadrupole instead of a radio | 12:54 | |
| chris_99 | haha | 12:55 |
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| chris_99 | i'm just looking at http://en.wikipedia.org/wiki/Coulter_counter again, could you possible just use a microfluidic slide to do it | 13:04 |
| nmz787 | yep | 13:06 |
| nmz787 | using those integrated spectrum analyzer chips | 13:06 |
| nmz787 | this or one of it's similar variants (found on ebay in modules, but can have cheap non-working crystals sometimes) http://www.analog.com/en/digital-to-analog-converters/direct-digital-synthesis-dds/ad5933/products/product.html | 13:07 |
| nmz787 | we got someone in here who was working on them | 13:07 |
| nmz787 | i can't remember their name | 13:07 |
| chris_99 | i'm confused, how does a spec analyser relate to this? or is that a response to something else | 13:07 |
| nmz787 | http://gnusha.org/logs/2014-11-21.log | 13:08 |
| nmz787 | nickjohnson: ^ | 13:08 |
| nmz787 | you were there chris_99 :P | 13:09 |
| nmz787 | you get a bode plot | 13:09 |
| nmz787 | which is your 'fingerprint' | 13:09 |
| nmz787 | consider your sample an antenna | 13:09 |
| nmz787 | or a signal filter | 13:09 |
| nmz787 | you get the response/lack-thereof of it | 13:09 |
| nmz787 | and for faster detection, you don't scan all the freqs, you use one or a range that is indicative/unique enough for your target of interest | 13:11 |
| delinquentme | kanzuragi dataframe = convert_start_time(dataframe) | 13:11 |
| nmz787 | and the common signal noises that can influence other freqs response | 13:11 |
| delinquentme | dis. does this rewrite that object ... ? | 13:11 |
| chris_99 | i thought you just detect 'pulses' nmz787, which are created as a change in resistance | 13:12 |
| nmz787 | you could probably also do something with fourier transforms, to use only square waves with diff slew-rate as input to the sample... since square waves are composed of infinite-series of sines | 13:14 |
| nickjohnson | That's me, but doing a bode plot is different from having a spectrum analyzer. | 13:14 |
| nickjohnson | nmz787: Square waves only have odd harmonics. A noise source is a more common approach. | 13:14 |
| nmz787 | chris_99: yeah but impedance is resistance but across more freqs | 13:14 |
| nmz787 | resistance is impedance at 0Hz | 13:14 |
| chris_99 | ah oh yeah it's impedance | 13:15 |
| nmz787 | nickjohnson: it was just an idea that came to mind... i'm no expert, but thanks for the tip for more investigation! | 13:15 |
| nmz787 | g2g drive into town | 13:15 |
| nickjohnson | nmz787: In theory if you feed in white noise, what you get out of the FFT is your response graph | 13:15 |
| chris_99 | nickjohnson, i see your project is funded :) | 13:22 |
| chris_99 | *product | 13:22 |
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| streety | the millipore scepter is not much more than a disposable microfluidic chip | 13:25 |
| streety | nice concept, I really should try to get our device up and running again | 13:26 |
| chris_99 | oh not seen that | 13:27 |
| chris_99 | so it sucks up the cells and does the coulter counting thing? | 13:27 |
| streety | yeah | 13:27 |
| chris_99 | neat | 13:27 |
| streety | our device doesn't suck though which is "apparently" fixed by a firmware update but we've lost the "usb2" cable that isn't like any usb cable I have | 13:28 |
| fenn | chris_99: the closest i've seen is brian hemond's paper "Development and performance of a miniature, low cost mass spectrometer" http://dspace.mit.edu/handle/1721.1/67584 http://fennetic.net/irc/mass_spectrometer_miniature_low_cost_brian_hemond.pdf although he doesn't provide any code or actual schematics (despite them existing in a non-public git repo here: http://web.mit.edu/bhemond/git/ | 13:29 |
| fenn | i'm guessing glass-blowing would be a main technology for the DIYer without a full machine shop | 13:30 |
| fenn | i dunno why the hell everyone immediately assumed you would be using a low cost DIY mass spec for proteomics | 13:33 |
| chris_99 | cheers fenn | 13:34 |
| chris_99 | that seems really interesting! | 13:35 |
| streety | I think I brought up proteomics - just wishful thinking | 13:37 |
| nickjohnson | chris_99: Yup! :) | 13:38 |
| fenn | one nice thing a mass spec can do is unambiguous detection of elements | 13:38 |
| nickjohnson | chris_99: Just finished redesigning part of the input frontend to support higher counting rates. :) | 13:38 |
| streety | what's the product? | 13:39 |
| chris_99 | whatcha mean by counting rate | 13:39 |
| nickjohnson | streety: kickstarter.com/projects/nickjohnson/tsunami | 13:40 |
| nickjohnson | chris_99: For frequency counting on the input | 13:40 |
| chris_99 | oh gotcha, cool | 13:40 |
| nickjohnson | The comparator is still limited - theoretically - to 4MHz, though I'm fairly sure it goes faster | 13:41 |
| nickjohnson | But I replaced the XOR IC with a greenpak (tiny little programmable gate array from silego) for an extra $0.08, and was able to add a 2 bit selectable divider to the frontend | 13:41 |
| nickjohnson | So the AVR pin gets the input frequency divided by 1, 16, 256, or 4096 | 13:41 |
| chris_99 | XOR IC, as in logical xor, or something else | 13:42 |
| nickjohnson | Logical xor | 13:43 |
| nickjohnson | The Tsunami does phase detection by XORing the input signal with the generated signal, then using an RC filter to take the average | 13:43 |
| chris_99 | dumb question, what does phase detection mean exactly | 13:45 |
| streety | looks like an interesting project - congrats on your funding success | 13:46 |
| nickjohnson | streety: Thanks :) | 13:46 |
| nickjohnson | chris_99: So, you generate a signal, feed it through some stuff (say, an audio amp), and get it back | 13:46 |
| nickjohnson | It'll be attenuated somewhat, and it'll also be delayed | 13:46 |
| nickjohnson | The delay, as a proportion of the waveform, is the phase shift | 13:46 |
| streety | would I be right in thinking it has some of the same use cases as the red pitaya that was on kickstarter as while back? | 13:47 |
| chris_99 | aha that makes sense | 13:47 |
| nickjohnson | Yes, but this is cheaper, lower frequency, and easier to program. | 13:47 |
| chris_99 | you could release a high freq. one too ;) | 13:47 |
| nickjohnson | chris_99: So, I was paying $0.42 for a dedicated single gate XOR IC. But I realised I could swap it out for a greenpak3 for $0.50, and have a little programmable logic array | 13:48 |
| nickjohnson | chris_99: I've thought about it. I know someone who's working on something similar in fact. But then you're competing with the red pitaya. | 13:48 |
| chris_99 | ahh | 13:48 |
| chris_99 | neat, what kind of stuff can you do with that programmable chip | 13:48 |
| chris_99 | is it similar to an FPGA | 13:48 |
| nickjohnson | Lots. It's got a bunch of lookup tables, a bunch of counters, some d-flipflops, some analog comparators | 13:48 |
| nickjohnson | Yes, just much smaller | 13:48 |
| nickjohnson | And one-time programmable | 13:48 |
| chris_99 | oh, it has eeprom then? | 13:49 |
| nickjohnson | I think of them as modern versions of GALs/PALs | 13:49 |
| nickjohnson | Yeah. All you need is the chip itself and one bypass cap. | 13:49 |
| chris_99 | that sounds really interesting | 13:49 |
| chris_99 | i'll check that out | 13:49 |
| nickjohnson | Here's the design I put together for the Tsunami: http://i.imgur.com/L6qsitL.png | 13:49 |
| nickjohnson | Oh, and the dev kit is $60, and comes with a bunch of samples. It's really easy to work with, too | 13:49 |
| chris_99 | oh is that the way you program it via a flow chart kind of thing? | 13:49 |
| nickjohnson | Yeah, pretty much | 13:50 |
| nickjohnson | One day when I have spare time, I'm going to write a VHDL compiler for it :) | 13:50 |
| nickjohnson | The registers are extensively documented | 13:50 |
| chris_99 | heh that'd be neat | 13:50 |
| chris_99 | what pin package does it have | 13:51 |
| nickjohnson | That's the only downside: they're tiny | 13:51 |
| chris_99 | bugger :( | 13:51 |
| nickjohnson | 0.4mm QFNs | 13:51 |
| chris_99 | i only have a normal soldering iron | 13:51 |
| nickjohnson | Do you have a hot air gun? | 13:51 |
| nickjohnson | (If not, why not? They're $20 on ebay :P) | 13:52 |
| chris_99 | no i don't heh, i should buy one | 13:52 |
| nickjohnson | You could do it with an iron, but a hot air gun would be easier | 13:52 |
| nickjohnson | I've been thinking about putting together little dip breakouts | 13:52 |
| chris_99 | with QFN don't you need to apply pasted | 13:52 |
| chris_99 | *paste | 13:52 |
| chris_99 | and reflow? | 13:52 |
| nickjohnson | They could be awesome for retro computing enthusiasts - emulate any <18 pin 7400 series IC. | 13:52 |
| chris_99 | heh that'd be cool | 13:52 |
| nickjohnson | Well, it helps. But you can instead tin the pads with your regular iron, add some flux, then heat it up with the hot air. | 13:52 |
| chris_99 | ah hadn't thought of that | 13:53 |
| chris_99 | i got a qfn adapter for an ADC i got | 13:53 |
| fenn | emulate any retro computer | 13:53 |
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| streety | are the cheap hot air guns worth it? how good are they for SMD? | 14:01 |
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| nickjohnson | streety: They're absolutely fine for SMD. | 14:02 |
| nickjohnson | I use mine all the time and feel no need to upgrade, personally. | 14:02 |
| nickjohnson | I noted with amusement that dave from eevblog uses one too :P | 14:02 |
| chris_99 | i just thought i do have one of those air guns we use for a BBQ sometimes, i dunno if i could use that though | 14:02 |
| streety | I might buy one and some cheap chips, give it a try | 14:03 |
| chris_99 | sounds like a good plan | 14:04 |
| streety | is it just take PCB, apply solder paste, place chip, apply heat? | 14:04 |
| nickjohnson | chris_99: I wouldn't risk it. Temperature control is vital. | 14:06 |
| nickjohnson | streety: Yes, though you can use regular solder and an iron, followed by flux, in place of paste | 14:06 |
| chris_99 | yeah i don't really want BBQ'd chips | 14:06 |
| streety | are there advantages to either approach? | 14:07 |
| nickjohnson | streety: Solder paste, IME, works best with a stencil, though you can get those nice and cheap, or with very careful dispensing. | 14:09 |
| nickjohnson | Solder and flux is self-limiting - you can't add too much solder - but it's slower and requires more attention than using a stencil | 14:10 |
| * nickjohnson -> bed | 14:10 | |
| chris_99 | i saw something really neat recently that autodispenced paste | 14:10 |
| chris_99 | night | 14:10 |
| streety | thanks nickjohnson, sleep well | 14:10 |
| nickjohnson | 'night | 14:10 |
| streety | I saw on dirtypcb that they offer stencils as an option, now I know why | 14:11 |
| chris_99 | https://www.kickstarter.com/projects/voltera/voltera-your-circuit-board-prototyping-machine/description | 14:11 |
| chris_99 | (not that i could afford to spend that much, but pretty cool) | 14:12 |
| streety | there was another one I saw at the maker faire in New York last year, pick and place together with applying solder paste | 14:14 |
| streety | again, not cheap | 14:14 |
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| streety | would love to see one at a local makerspace though | 14:14 |
| nickjohnson | chris_99: https://www.tindie.com/products/Pieco/paste-press/ | 14:15 |
| chris_99 | yeah i've seen the guns :) | 14:15 |
| fenn | this looks like the sort of thing one could easily make on a 3d printer | 14:17 |
| chris_99 | mmm | 14:17 |
| chris_99 | it does | 14:17 |
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| t12 | here have some troll bait | 14:26 |
| t12 | http://amormundi.blogspot.com/2012/12/is-transhumanism-racist.html | 14:26 |
| andytoshi | whois carissa | 14:29 |
| andytoshi | oops, i didn't mean to draw attention to quiet people | 14:29 |
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| carissa | I am Bryan's sister, just here lurking. | 14:35 |
| andytoshi | hi carissa. i was at lunch with bryan today, he mentioned you were here | 14:36 |
| andytoshi | we have met before, i'm andrew, i had thanksgiving with your family | 14:36 |
| kanzure | t12: please no | 14:38 |
| kanzure | dae carrico is a known moron and must be avoided at all costs | 14:39 |
| kanzure | *dale carrico | 14:39 |
| kanzure | t12: and also i'll kickban you, so that is extra incentive to not bother us with dale carrico things | 14:40 |
| t12 | sorry was just going for entertainment! | 14:41 |
| kanzure | that's some weird entertainment man | 14:41 |
| kanzure | you should also avoid james hughes | 14:42 |
| t12 | you could make tumblr meme generator out of | 14:47 |
| t12 | http://upload.wikimedia.org/wikipedia/commons/thumb/c/cd/James-Hughes---rays.jpg/1024px-James-Hughes---rays.jpg | 14:47 |
| t12 | very the correct aesthetic | 14:47 |
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| chris_99 | .title https://www.youtube.com/watch?v=1gu3z7w4Vc8 | 14:55 |
| yoleaux | Festo – eMotionButterflies (English) - YouTube | 14:55 |
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| fenn | very appropriate that the image has gratuitious noise added to it | 15:02 |
| fenn | t12 i want to frame that photo of the mass spec core in the bath tub | 15:04 |
| t12 | i can take a better one | 15:05 |
| fenn | tell people it's high concept art photography | 15:05 |
| t12 | though i kinda cleaned the bathtub up | 15:05 |
| t12 | i have some nice pictures of it somewhere | 15:06 |
| t12 | hum no full length ones | 15:18 |
| t12 | https://www.dropbox.com/sc/0augzr1j7q5tfhb/AACjOrQ4smQzyX8lta3qR5d3a | 15:18 |
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| nmz787 | that tri-quad-pole in a bathtub might go on a trial run as my work desktop background | 15:46 |
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| t12 | https://www.dropbox.com/s/j06rfhyi1el1r5x/20150329-20150329-_3290007.jpg?dl=0 | 16:34 |
| t12 | heres a better picture | 16:34 |
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| thundara | t12: https://lh5.googleusercontent.com/-o0OhYkHi55k/VMHvlQRqsNI/AAAAAAAABis/avWx66KVVyM/w2960-h1665-no/20150121_162914.jpg The one I want to dissect | 16:36 |
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| t12 | qstar? | 16:42 |
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| t12 | they're fun to take apart | 16:44 |
| t12 | if its destined to the trash save the stainless main chamber | 16:44 |
| t12 | they scrap out for alot | 16:44 |
| t12 | also the electronics are likely resaleable to a 3rd party repair shop | 16:45 |
| t12 | i never had luck actually doing that, but also didnt try too hard | 16:45 |
| t12 | rebuild houses will buy turbos | 16:48 |
| t12 | and the controllers | 16:48 |
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| kiba-immle | hi | 16:56 |
| kiba-immle | how to request a paper from the paper bot? | 16:56 |
| kiba-immle | !request https://www.thieme-connect.com/DOI/DOI?10.1055/s-2000-9236 | 16:56 |
| kiba-immle | .request https://www.thieme-connect.com/DOI/DOI?10.1055/s-2000-9236 | 16:56 |
| kiba-immle | :-/ | 16:56 |
| kiba-immle | !paperbot https://www.thieme-connect.com/DOI/DOI?10.1055/s-2000-9236 | 16:57 |
| kiba-immle | .paperbot https://www.thieme-connect.com/DOI/DOI?10.1055/s-2000-9236 | 16:58 |
| kiba-immle | !fetch https://www.thieme-connect.com/DOI/DOI?10.1055/s-2000-9236 | 16:58 |
| kiba-immle | shiiiit | 16:58 |
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| fenn | paperbot: https://www.thieme-connect.com/DOI/DOI?10.1055/s-2000-9236 | 17:03 |
| paperbot | http://diyhpl.us/~bryan/papers2/paperbot/afd2fb9d816093c8e49aaf45ef2b9d66.txt | 17:04 |
| n_bentha | thanks fenn! it's been a while and i had forgotten >.< | 17:04 |
| n_bentha | paperbot: https://www.thieme-connect.com/products/ejournals/html/10.1055/s-2000-9236 | 17:05 |
| paperbot | http://diyhpl.us/~bryan/papers2/paperbot/1236ceffe65c63713f1e0ec8ef68729c.txt | 17:06 |
| fenn | paperbot: http://www.thieme-connect.com/products/ejournals/pdf/10.1055%2Fs-2000-9236.pdf | 17:06 |
| paperbot | http://diyhpl.us/~bryan/papers2/paperbot/4153005c741d4f711f445a1a45370b09.txt | 17:07 |
| fenn | paperbot: http://www.thieme-connect.com/products/ejournals/pdf/10.1055/s-2000-9236.pdf | 17:07 |
| paperbot | http://diyhpl.us/~bryan/papers2/paperbot/952c5c05aa68b0065fcfa2d681bad700.txt | 17:08 |
| fenn | i guess that's a bad url | 17:08 |
| fenn | also paperbot can't get it, sorry | 17:09 |
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| n_bentha | ok thanks | 17:11 |
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| thundara | t12: Good to know, haha | 17:16 |
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| n_bentha | paperbot: http://www.sciencemag.org/cgi/pmidlookup?view=long&pmid=19892975 | 19:31 |
| n_bentha | hey nootropics are in the discussion! awesome :D | 19:32 |
| paperbot | http://diyhpl.us/~bryan/papers2/paperbot/Hematopoietic%20Stem%20Cell%20Gene%20Therapy%20with%20a%20Lentiviral%20Vector%20in%20X-Linked%20Adrenoleukodystrophy.pdf | 19:32 |
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| nmz787 | met this guy's son tonight http://www.mse.ncsu.edu/research/labean/ | 20:29 |
| kanzure | .title | 20:40 |
| yoleaux | LaBean Research Group | 20:40 |
| kanzure | "We use biopolymers" | 20:40 |
| kanzure | looks like lot of winfree/rothemund stuff | 20:41 |
| nmz787 | paperbot: http://link.springer.com/chapter/10.1007/978-3-540-24635-0_12 | 20:42 |
| paperbot | http://diyhpl.us/~bryan/papers2/paperbot/b2f98ddd0b9c24c5a5528fe660670b32.txt | 20:43 |
| nmz787 | yeah, you recognize any of the papers? | 20:43 |
| kanzure | not exactly, but the topics are quite similar, just more heavy metals i guess | 20:44 |
| nmz787 | http://diyhpl.us/~nmz787/pdf/Aspects_Of_Molecular_Computing.pdf | 20:48 |
| nmz787 | DNA-crypto chapter therewithin | 20:48 |
| nmz787 | one-time pads | 20:52 |
| nmz787 | hmm | 20:52 |
| kanzure | not really one-time, you probably have millions of dna molecules floating around with the same sequence | 20:53 |
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| nmz787 | "Finally, adding ligase enzyme results in a continuous reporter strand R that runs through the entire assembly. If bi = ai ⊕ a i, for i = 1,...,n, then the reporter R = a1a2 ...an.a 1a 2 ...a n.b1b2 ...bn. The reporter strand is shown as a dotted line in Figure 3. This strand may be extracted by first melting apart the hydrogen bonding between strands and then purifying by polyacrylamide gel electrophoresis. It contains the input ... | 21:01 |
| nmz787 | ... message, the encryption key, and the ciphertext all linearly concatenated. The ciphertext can be excised using a restriction endonuclease if a cleavage site is encoded between the a0 and b1 tiles. Alternatively the reporter strand could incorporate a nick at that point by using an unphosphorylated oligo between those tiles. The ciphertext could then be gel purified since its length would be half that of the remaining sequence. This may ... | 21:01 |
| nmz787 | ... then be stored in a compact form and sent to a destination." | 21:01 |
| nmz787 | Fig 4 is pretty cool | 21:04 |
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| n_bentha | paperbot: http://onlinelibrary.wiley.com/wol1/doi/10.1111/cen.12730/full | 22:03 |
| paperbot | http://diyhpl.us/~bryan/papers2/paperbot/d2f63091c82e303d33e97b9356f12ac3.txt | 22:04 |
| n_bentha | paperbot: http://onlinelibrary.wiley.com/doi/10.1111/cen.12730/epdf | 22:05 |
| paperbot | http://diyhpl.us/~bryan/papers2/paperbot/7cd38a745c8de3f2459dc73dae607a57.txt | 22:05 |
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| nmz787 | .tell chris_99 congrats man! http://hackaday.com/2015/03/29/measuring-alcohol-content-with-time-of-flight-sensors/ | 22:20 |
| yoleaux | nmz787: I'll pass your message to chris_99. | 22:20 |
| nmz787 | .tell chris_99 this seems like the best/most-constructive comment http://en.wikipedia.org/wiki/Oscillating_U-tube | 22:23 |
| yoleaux | nmz787: I'll pass your message to chris_99. | 22:23 |
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