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maaku | kanzure: anyone to see in boston? | 00:40 |
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maaku | boston/cambridge | 00:40 |
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abetusk | I'm in Somerville, not that I'm worth seeing | 05:54 |
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kanzure | maaku: djat, abetusk, ed boyden, adam marblestone, david dalrymple (although he might have moved to the bay area because he's a traitor), heath matlock (ybit in here), david vorick (taek), bosslab folks, fablab folks, mit media lab folks, http://syntheticneurobiology.org/ these people, mit dci people if you can tolerate them (perhaps joi ito at least). | 06:45 |
kanzure | taek will know more people on the cryptography and computation side of things. | 06:47 |
kanzure | neil gershenfeld i guess. | 06:52 |
kanzure | george church if you can flag him down ever. | 06:52 |
kanzure | not sure about connectomics people but there should be at least two or three of those wandering around boston somewhere | 06:53 |
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kanzure | need some name suggestions for the electroporator (if i was to pick the name, that is). "cell membrane disruptor" "membrane scrambler" "transgenetic doohickey".. meh. | 07:50 |
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kanzure | "cultural shocker" | 07:52 |
kanzure | "brane piercer".. | 07:52 |
kanzure | damn, probably not going to find much better than "culture shock" | 07:53 |
kanzure | "culture wand" | 07:55 |
JayDugger | electro-dialator? | 08:02 |
JayDugger | Yeah, I'll stop. All the rest of my ideas are starting to sound like sex toys. | 08:02 |
JayDugger | Electric key? SFW, and accordingly puny. | 08:03 |
JayDugger | Transfection funnel? (Not too sure about that verb-turned-adjective. Might lack accuracy and euphony.) | 08:05 |
JayDugger | <ADJECTIVE> <NOUN>, where <NOUN> ∈ {disruptor, doohickey, key, nozzle, scrambler, shocker, shooter, wand} and <ADJECTIVE> ∈ {brane, cell membrane, cultural, culture, electro, electric, membrane, transgenic, transfection}. | 08:09 |
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maaku | this adam marblestone character looks interesting. | 09:56 |
kanzure | maaku: http://diyhpl.us/~bryan/papers2/neuro/Designing%20scalable%20biological%20interfaces%20-%20Marblestone.pdf | 09:57 |
maaku | was just reading that | 09:57 |
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maaku | catern: trying to decide if I should make the jump and get a pebble time 2, for the purpose of hacking to 24/7 record sensors | 10:23 |
maaku | at the same time for $24 this device has extremely good audio quality : https://www.amazon.com/Weefun-Wristband-Rechargeable-Reduction-Interview/dp/B01B79K0ZW/ref=sr_1_5?ie=UTF8&qid=1465752208&sr=8-5&keywords=voice+record+wrist | 10:24 |
maaku | it just doesn't sync over BLE :( | 10:24 |
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jrayhawk | http://www.tandfonline.com/doi/full/10.1080/15592294.2015.1062207 attn: fenn and other larmarckian denialists | 10:29 |
jrayhawk | er, lamarckian | 10:30 |
catern | maaku: that looks perfect! | 10:47 |
catern | why is BLE needed, since it has storage capacity for 48 hours and battery capacity for 20 hours? | 10:48 |
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maaku | catern: I'm looking to the more ambitious goal of analyzing this in real time | 11:30 |
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maaku | catern: i ordered one of these weefun devices | 12:05 |
catern | ah, that is ambitious, but interesting | 12:06 |
maaku | i do eventually want to get this stuff processed in real time and use some sort of feedback to the wearer | 12:06 |
catern | it's unfortunate... i don't think i'll actually be able to do this once out of university | 12:06 |
catern | since i don't think my job would look kindly upon recording everything being said around me | 12:07 |
kanzure | speak for yourself | 12:07 |
kanzure | high-resolution logs from meetings and phone calls has come in handy a number of times on my end | 12:07 |
maaku | if nothing else it would be nice to have on at home recording all the cute things my kids say | 12:07 |
maaku | kanzure: what we need is some way of automating extraction of information from that though | 12:08 |
catern | i guess if i had separate devices recording at work and at home | 12:08 |
catern | maaku: i think grep in itself is pretty handy | 12:08 |
kanzure | before it can be automated there must be a manual method, and i don't think there's a manual method for that either.. | 12:08 |
maaku | agreed. your information is not sufficiently structured | 12:09 |
kanzure | speakers are also bad at structuring information, fwiw :) | 12:09 |
maaku | not a criticism obviosuly as you are doing a better job than any of us, but you're lacking a higher level abstraction on top of that information | 12:10 |
maaku | which would be used to decide when to prompt you with info, or 'remind this person about x,y,z' | 12:10 |
kanzure | often to me it sounds like endles strings of word spaghetti. took me a while to realize that lots of people don't communicate to inform but rather for uh other reasons.. | 12:10 |
maaku | going down this path it quickly becomes an ai-complete problem, which is not surprising since what you're really doing is automating a personal secretary | 12:11 |
kanzure | haha secretary.... maybe. that's kind of overstating the job of a secretary though. | 12:11 |
maaku | personal assistant? | 12:12 |
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kanzure | btw you should also put some thought into my meetlog problems (described in recent backlog) http://gnusha.org/logs/2016-06-10.log (see near 15:55) | 12:12 |
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kanzure | compounding benefits of transcripts vs. compounding benefits of correctly (and more timely) routing information and knowledge to various people | 12:13 |
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kanzure | yashgaroth: gimme a creative and crazy name for an open-source electroporator device project thing | 12:15 |
kanzure | candidates so far are "open electroporator", "culture wand", "shock wand", "culture shock", "membrane disruptor", "bishop cell zapper". | 12:16 |
yashgaroth | if only "shock level 1" worked, but it doesn't really | 12:17 |
yoleaux | 31 May 2016 17:17Z <nmz787_i> yashgaroth: you could have an identical set of wells below the wells you're talking about, with gel sandwiched between, vertically... then electrophoresis from the top well down to the bottom | 12:17 |
yashgaroth | may 31st?! | 12:18 |
kanzure | http://gnusha.org/logs/2016-05-31.log | 12:19 |
yashgaroth | I can't speculate on how the electronics on that would work, it's like that field of infinite resistors problem; but if none of the proteins co-migrate with the DNA, or have an acidic isoelectric point, mmmmaybe | 12:23 |
kanzure | infinite field of resistors, rather :) | 12:23 |
yashgaroth | well yes, I suppose infinite resistors are easier to calculate for | 12:24 |
yashgaroth | also I realized before that with the TdT method you're synthesizing 5'-3' which makes it really annoying to generate a template for nicking + polymerase since the template is in the wrong orientation | 12:24 |
yashgaroth | unless you're not amplifying off the template but that's also a huge pain, oh well | 12:25 |
yashgaroth | I mean you could make the synthetic long enough to hairpin and wrap back around and then hit it with a couple of nicking enzymes first, but that's like quintupling the length you need to synthesize | 12:26 |
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kanzure | john likes the name "shock wand" | 14:04 |
kanzure | yashgaroth: any reasons to not go with that? | 14:04 |
yashgaroth | of those listed I prefer that one, should be fine | 14:04 |
kanzure | not too similar to *ahem* the magic wand? | 14:06 |
yashgaroth | I think "shock" is enough to block mental associations but there's no real way of telling | 14:07 |
kanzure | i'm pretty sure people call that "electricity play" or something | 14:07 |
yashgaroth | oh yeah check the first google result, nm | 14:08 |
kanzure | hahah | 14:08 |
yashgaroth | "open electroporator" might be okay, plus if you preface 'open' onto any lab equipment you're guaranteed six figures on kickstarter | 14:10 |
kanzure | not this guy though. he is not good at marketing at all. and kickstarter is mostly about marketing. | 14:10 |
kanzure | i could pay a firm to do his kickstarter campaign i guess, but basically i have struck an agreement with him where i'm funding the project so that he doesn't have to bother with crowdwhatever | 14:11 |
yashgaroth | so is it like cuvette-based or what? | 14:11 |
kanzure | http://cibolo.us/pipermail/open_electroporator/2016-June/000071.html | 14:12 |
kanzure | image http://cibolo.us/pipermail/open_electroporator/attachments/20160602/40066a2a/attachment-0001.jpg | 14:12 |
kanzure | yashgaroth: also check the long-form proposal sent yesterday http://cibolo.us/pipermail/open_electroporator/attachments/20160611/03ec46ed/attachment-0001.odt | 14:13 |
kanzure | it was also discussed recently in here http://gnusha.org/logs/2016-06-03.log | 14:14 |
yashgaroth | hmm never seen a pipetrode-based one before | 14:18 |
kanzure | http://www.tritechresearch.com/shop/items/CG-1.JPG | 14:18 |
kanzure | http://www.tritechresearch.com/CG-2.html | 14:19 |
kanzure | "mammozapper" alright well whatever name gets picked, it needs to be better than "mammozapper" | 14:19 |
yashgaroth | and not look like a purple alien dildo | 14:20 |
yashgaroth | but yeah if the pipet-based system already works then it's just electrons and stuff...wait did he mention anything about the actual pipetting action, like drawing and dispensing the cells? | 14:24 |
yashgaroth | oh that's the pull lever thing, okay | 14:26 |
kanzure | "Soon after this is done as a full manual machine, (see the lever on the grip?), I'll be searching for teeny gear motors to push on a length of tubing for the pipette expel and draw in functions. The it will be an automation tool also." | 14:26 |
yashgaroth | would be nice if there was a version with the same electronics but worked with standard cuvettes, since those are infinitely more popular with everyone | 14:31 |
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yashgaroth | ah good he mentions starting with regular cuvettes, especially if tritech is the only people who do the pipetrodes | 14:35 |
kanzure | apparently the tritech person has been responsive and cooperative | 14:37 |
yashgaroth | "hi I'd like to make a lower-priced version of your product, will you be the sole existing supplier of the consumables? great" | 14:38 |
kanzure | low price is not always something to worry about | 14:39 |
kanzure | usually folks who are doing low price work will not be great at marketing because they can't afford it | 14:39 |
kanzure | or, the low price folks wont be seeking the big customers who are incapable of buying things under $5000 | 14:39 |
yashgaroth | depends what proportion of your user base will be diybio people, versus poor labs, of which there are more every day | 14:42 |
yashgaroth | the open qPCR machine has been popping up in academic labs when they actually manage to manufacture one, and it apparently works fine for $3k vs the usual $30k floor for qPCR | 14:43 |
kanzure | they are lethargic? or manufacturing problems? | 14:43 |
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yashgaroth | I'll give them the benefit of the doubt, but they claim QC issues in china what a surprise | 14:44 |
kanzure | well we have someone in china now as soon as that ultrasound project starts up (i have a call on monday with shanghai about their team) | 14:45 |
yashgaroth | I'm not saying they can't do good stuff, but I've had reliability issues even with new qPCR setups, it needs a lot of precision | 14:46 |
kanzure | what's the precision for? | 14:47 |
kanzure | photon counting? | 14:47 |
yashgaroth | looking for very small variations in fluorescence in real time with a lot of noise, some temperature variability on the reactions | 14:47 |
kanzure | hmm. | 14:48 |
yashgaroth | even the expensive machines are just LED + CCD with peltiers for temperature, so it can be done | 14:48 |
kanzure | what about better fluorophores | 14:48 |
kanzure | i guess that's victim blaming, heh | 14:48 |
yashgaroth | trading off noise for signal there, modern ones are quite bright and photostable | 14:48 |
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kanzure | emailing jojack about "but what about the sex toys" was a mistake, his response is completely predictable | 14:59 |
yashgaroth | why, was it just "everything is a sex toy now, just roll with it"? | 14:59 |
kanzure | pretty much | 15:00 |
kanzure | "Lol you should embrace the sex toy connotations" | 15:01 |
yashgaroth | well, if you consider it from the perspective of genetic transfer, the parallels write themselves | 15:01 |
kanzure | and then he listed out some names, so there's that at least. | 15:01 |
kanzure | "Bio blaster genezapper" | 15:02 |
kanzure | "shockswapper" | 15:02 |
yashgaroth | ooh bio blaster | 15:02 |
kanzure | jojackulator | 15:02 |
kanzure | plasmidifier? | 15:03 |
yashgaroth | also good | 15:04 |
kanzure | rotpatcher | 15:05 |
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maaku | kanzure: fenn was right. "you eventually need to actually do a calculation | 15:13 |
maaku | i mean right now you're doing that in your head -- see person, look up what you need to talk about or whatever, and use your index to acquire info | 15:14 |
maaku | i don't really know what algorithm you're using or wanting to use with this data, but right now it's you that's doing it | 15:14 |
maaku | so some clarity on the goal here would better inform how it can be automated | 15:14 |
maaku | (and it is actually a project i'm interested in, related to why i'm looking into this pervasive personal recording gear) | 15:15 |
kanzure | well i think at the moment i'm out of ideas because of a failure to think of a relevant metric | 15:16 |
kanzure | it's not easy to write a precise definition of "correctly routing information and knowledge to the right people at the right time (or earlier)" | 15:16 |
kanzure | and perhaps that's too ambiguous | 15:16 |
maaku | yeah that's just hiding all the complexity in "correctly" | 15:18 |
maaku | If you have the past conversational history with a person, you should be able to build a model of "relevance" to that person for other ideas | 15:20 |
maaku | then sort all ideas by relevance, filter out those already discussed, and start from the top | 15:21 |
maaku | As to how you actually do that, it sounds like some deep learning NLP, and some sort of complicated topology comparisons in thought space ... maybe pasky has some ideas | 15:22 |
maaku | or jcorgen... where'd he go anyway? | 15:22 |
kanzure | hm i guess we should ping him. i was going ot ask if you had done a hangout with him now that you're back stateside. | 15:23 |
maaku | not yet, i should | 15:24 |
kanzure | here is a brief sketch of one possible direction | 15:24 |
kanzure | there are two phases | 15:24 |
kanzure | one is manually specifically marking individuals as subscribed to certain overarching topic feeds, with relevancy weights or something (yes there are ways to do auto classification here, sure) | 15:25 |
kanzure | second is to daily run something that monitors for discussed tags/conversations that seem like they are relevant to each topic/stream, and then put those topics into a queue and select some people subscribed to that stream who have not been talked iwth recently on those subjets | 15:25 |
kanzure | *subjects | 15:26 |
maaku | makes sense | 15:26 |
kanzure | another thing i would like eventually is something where i could "pin" two people together so that i could say "i talk with maaku a lot about neuroscience, and to the extent that i do, it would be good to be reminded to also equally talk with notmaaku about those same topics" | 15:28 |
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pasky | i tried to find the context for this question but got lost in the backlog | 15:29 |
kanzure | pasky: i have a giant database of every conversation i've had since 2009. each conversation has at least 5 to 10 tags, sometimes more. the goal is to come up with some metric and then automation around "helping kanzure figure out which people to be speaking with and which things to be informing them about" e.g. because it's helpful to synchronize sooner rather than later due to forgetfulness or whatever. | 15:31 |
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maaku | pasky: and I've taken this as a special case of a larger problem -- making sure the right information is available at the right moment to keep us all productive | 15:41 |
maaku | i had some musings above about using NLP to assign relevance scores for (person, info) pairings | 15:41 |
kanzure | i want to avoid false negatives like "oops i forgot to tell andytoshi about this 32-byte infinite multisig scheme that i was talking with some random person about" | 15:55 |
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justanotheruser | is pyquante the best chemical simulation library for python? | 17:42 |
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kanzure | justanotheruser: i think the blueobelisk people would have very strong opinions about that (and they would probably be accurate) | 19:25 |
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justanotheruser | o_o https://upload.wikimedia.org/wikipedia/commons/a/a6/BlueoneliskDeps_CCBY2.0.jpg | 19:29 |
kanzure | oh yeah it's a bit of a spaghetti hunt, have fun with that | 19:30 |
kanzure | if your hunt leads back to me, then all i can suggest is start over from the beginning | 19:30 |
justanotheruser | hah | 19:30 |
justanotheruser | they should have come up with a better name so my results don't keep going to a fictional dorm in yu-gi-oh | 19:31 |
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maaku | justanotheruser: depends on the chemical simulation you are doing | 19:59 |
maaku | none are actually implemented in Python though | 19:59 |
kanzure | some of the CHARMM stuff and whatever probably have python bindings, though | 20:01 |
justanotheruser | maaku: it's all cython in pyquante. I assume you've used it? | 20:01 |
kanzure | nanoengineer had lots of python bindings for various molecular dynamics things | 20:01 |
kanzure | yes, yes, everyone has used cython (and i'm technically a cython author i think) | 20:02 |
kanzure | https://github.com/cython/cython/commits?author=kanzure | 20:02 |
kanzure | i guess not. well anyway... | 20:02 |
justanotheruser | no I mean I assume he's used pyquante | 20:03 |
* kanzure sleeps | 20:05 | |
justanotheruser | What does NE do? n-body simulations I assume? calculating the potential energy surface? | 20:07 |
justanotheruser | problem with pyquante is it seems to not be actively developed is written in python2 https://github.com/rpmuller/pyquante2 | 20:08 |
maaku | justanotheruser: never have used pyquante | 20:11 |
maaku | justanotheruser: really depends on what you are looking to simulate | 20:12 |
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justanotheruser | energy minimization, binding affinity, reaction simulations | 20:16 |
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fenn | .title https://www.youtube.com/embed/Ypb7jG3FgWA | 20:49 |
yoleaux | Open qPCR Kickstarter Video - YouTube | 20:49 |
fenn | they did a pretty good job on the video at least | 20:49 |
fenn | https://angel.co/chai | 20:53 |
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nmz787 | sup | 22:37 |
nmz787 | .tell chris_99 hey do you know any good chips with a Programmable Gain Amplifier (PGA) for pre-ADC? | 22:39 |
yoleaux | nmz787: I'll pass your message to chris_99. | 22:39 |
nmz787 | .tell chris_99 preferably something that can run micropython :/ | 22:41 |
yoleaux | nmz787: I'll pass your message to chris_99. | 22:41 |
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