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Biological Science Symposia

B01 The R.P. Apkarian Memorial Symposium on Cryo-HRSEM

Ongoing Developments of the Cryo-SEM/STEM Technique; AC Robins; E A Fischione Instruments, Inc [24]

Imaging Macromolecules and Viruses in a Hydrated State Using a Field Emission Scanning Electron Microscope (FESEM); CA Ackerley, C Nielsen; Hospital for Sick Children; G Rodek; SPI Supplies [25]

Field Emission In-Lens Scanning Electron Microscopy of the Nuclear Envelope and Other Cellular Structures; MW Goldberg, J Fi_erov‡; Durham University; TD Allen; The Institute of Cancer Studies, University of Manchester [26]

375

Cryo-EM Methods for the Structural Analysis of Biomimetic Materials based on Peptides and Proteins;VP Conticello; Emory University [188]

377

Cryo-Planing Vs Freeze Fracture: Sample Preparation for Cryo-HRSEM; IY Chang, D Joester; Northwestern University [189]

379

Serial Block Face-Scanning Electron Microscopy of the Developing Rat Brain Exposed to Ketamine Reveals Changes in Mitochondrial Ultrastructure; T Eustaquio, C Wang, CK Dugard; U S Food and Drug Administration, Center for Devices and Radiological Health; F Liu, W Slikker, MG Paule,P Howard, AM Paredes; U S Food and Drug Administration, National Center for Toxicological Research et al [190]

381

Architecture of Pancreatic Islets Imaged by Serial Block Face SEM; RD Leapman, MA Aronova,A Shomorony, CR Pfeifer, JD Hoyne, GN Calco, BC Kuo, G Zhang; National Institute of Biomedical Imaging and Bioengineering, NIH et al [191]

383

Correlating Cryo-Electron Microscopy Methods for Structural Studies of Bacteria; ER Wright, H Yi, JV Taylor, RC Guerrero-Ferreira, CM Hampton; Emory University [192]

181

Characterization of a Crude Oil Emulsion by Cryo Electron Microscopies; AR Behzad, DH Anjum; King Abdullah University of Science and Technology [91]

183

Cryo-SEM and Raman Spectroscopy Study of the Involvement of Polyhydroxyalkanoates in Stress Response of Bacteria; V Krzyzanek, K Hrubanova, O Samek; Institute of Scientific Instruments of the ASCR; S Obruca, I Marova; Brno University of Technology; S Bernatova, M Siler, P Zemanek; Institute of Scientific Instruments of the ASCR [92]

185

Comparison of Sample Preparation Methods for Analysis of Mucus-Secreting Colon Cancer Cells by Scanning Electron Microscopy; X Wang, R Bleher, V Backman, GS Shekhawat, VP Dravid; Northwestern University [93]

187

Monitoring of Multilayered Bacterial Biofilm Morphology by Cryo-SEM for Raman Spectroscopy Measurements; K Hrubanova, S Bernatova, O Samek, M Sery, P Zemanek; Institute of Scientific Instruments of the ASCR; J Nebesarova; Biology center ASCR; F Ruzicka; Masaryk University, V Krzyzanek; Institute of Scientific Instruments of the ASCR [94]

189

Morphology of Organic Matrix of Human Enamel; VM Dusevich, JD McGuire, JP Gorski, Y Wang, MP Walker; UMKC School of Dentistry [95]

B02 To the Rhizosphere - and Beyond!

707

The use of Microcontact Printing of Thiols to Evaluate Attachment of Xylella fastidiosa Under Distinct Conditions of Calcium Availability; B Leite, L De La Fuente; JEOL USA Inc [354]

709

Rhizomes and Roots of Rare Arctic-Alpine Snowfield Plants on the Edges of Retreating Snowfields at Glacier National Park, Montana; ME Apple, MK Ricketts, LG Carlson; Montana Tech of the University of Montana [355]

711

Impact of Phenazine-1-carboxylic acid upon Biofilm Development in the Rhizosphere of Dryland and Irrigated Wheat; MK LeTourneau, MM Marshall; Washington State University; LS Thomashow; United States Department of Agriculture Agricultural Research Service; JB Harsh; Washington State University [356]

713

Imaging Ventral Cell Plate Formation in Guard Cells; X Qi, KU Torii; University of Washington [357]

1485

Further Differences in Biochemical Composition of Roots of Ni-Hyperaccumulating and Non- Hyperaccumulating Genotypes of Senecio coronatus; AD Barnabas, J Mesjasz-Przybylowicz; IThemba LABS, National Research Foundation; I Yousef; SESAME Synchrotron; P Dumas;SOLEIL Synchrotron; F Jamme; SOLEIL Synchrotron; PT Sechogela; iThemba LABS, National Research Foundation; WJ Przybylowicz; iThemba LABS, National Research Foundation [741]

1487

The Use of Fluorescent In Situ Hybridization in Bacterial Detection in Mycorrhizal Networks;W Chrisler, G Orr, A Dohnalkova, L Pa_a-Toli_; Pacific Northwest National Laboratory; G Stacey; University of Missouri; C Chun, M Sadowsky; University of Minnesota [742]

1489

A Multiscale Approach to Understanding Calcium Toxicity in Australian Proteaceae; PL Clode, P Hayes; University of Western Australia; N Honvault; Institut Polytechnique Lasalle Beauvais; H Lambers; University of Western Australia [743]

1491

Investigation of structure-function relationship of long-distance transport in plants: New imaging tools to answer old questions; M Knoblauch, D Mullendore, D Froelich, S Beecher, TJ Ross-Elliott,J Knoblauch; Washington State University [744]

861

The Importance of Location, Location, Location: As True for Rhodococcus fascians as for Real Estate; T Sawyer, M Putnam; Oregon State University [431]

863

Investigation into the Stabilization of Soil Organic Matter by Microbes; AC Dohnalkova; Pacific Northwest National Laboratory [432]

865

The Development and Structure of Cornalean Flowers and Fruits; T Sawyer, B Atkinson, G Rothwell, R Stockey; Oregon State University [433]

867

Motphology, Anatomy, and Development of Cunninghamia lanceolata (Cupressaceae) Pollen Cones;T Sawyer, B Atkinson, G Rothwell, R Stockey; Oregon State University [434]

869

Why Phragmites australis canes grown in Udono reed bed are the best materials for the reeds of Japanese wind instrument ÔhichirikiÕ ? -A structural and biomechanical study-; M Kawasaki,T Nobuchi; JEOL USA Inc; Y Nakafushi, M Nose; University of Toyama; M Shiojiri; Kyoto Institute of Technology [435]

871

Analysis of Plant Responses to Titanium Dioxide (TiO2) Nanoparticles; K Smith, K Ghoshroy, S Ghoshroy; University of South Carolina [436]

B04 Advances in Specimen Preparation and Correlative LM-EM (CLEM) of Biological Samples

385

Placing Molecules in a Cellular Context Using Light, Electron and X-ray Microscopy; LM Collinson; Francis Crick Institute [193]

387

Important steps in a Correlative Light Electron Microscopy Experiment; P Verkade; Wolfson Bioimaging Facility, Schools of Biochemistry and Physiology Pharmacology [194]

389

Correlating Microscopies From Differing Imaging Modalities: From Experimental Design to Alignment and Overlay of Images; DR Keene, SF Tufa; Shriners Hospital for Children [195]

531

Detection of GFP-labeled Proteins by Electron Microscopy; RG Parton, T Hall, N Ariotti; The University of Queensland [266]

533

Systematic characterization of fluorophore behavior in the presence of electron microscopy sample preparation reagents; H Li, DM Jorgens; Oregon Health Science University; L Wang, RM Strongin; Portland State University; JW Gray, SL Gibbs; Oregon Health Science University [267]

535

Studying Membrane Trafficking in Toxoplasma gondii Using Correlative Light and Electron Microscopy (CLEM); R-C Hsia, JE Strong; University of Maryland Baltimore; JD Romano, I Coppens; Johns Hopkins University [268]

537

New CLEM Method to Reveal Ultrastructural Reorganization in the Host Cell during Coronavirus Infection; G Kiss, C Bouchet-Marquis, L Pullan; FEI Company; D Keene; Shriners Hospital, Micro- Imaging Center; BW Neuman; University of Reading [269]

539

On the road to large volumes in LM and SEM: New tools for Array Tomography; I Wacker, W Spomer; Cryo EM, Centre for Advanced Materials, UniversitŠt Heidelberg; A Hofmann, U Gengenbach; Institute for Applied Computer Science, Karlsruhe Institute of Technology; M Thaler; Carl Zeiss Microscopy GmbH; L Ness, P Brey; Boeckeler Instruments, Inc, R Schršder; Cryo EM, CellNetworks, BioQuant, UniversitŠtsklinikum Heidelberg [270]

1115

Quick Freeze Substitution Processing of Biological Samples for Serial Block-face Scanning Electron Microscopy; RI Webb, RE Webb; University of Queensland [557]

1117

The Acquisition of large datasets with serial-block face scanning electron microscopy following light microscopy image acquisition: workflows from sample preparation to image acquisition; C Genoud, AA Wanner, RW Friedrich; Friedrich Miescher Institute for Biomedical Research [558]

1119

In Situ Tomography of Membrane Proteins Enabled by Advanced Cryo-FIB Sample Preparation and Phase Plate Imaging; M Schaffer, BD Engel, M Wehmer, T Gupta, E Sakata, T Laugks, J Mahamid, W Baumeister; Max Planck Institute of Biochemistry et al [559]

1121

Imaging of Vitrified Biological Specimens by Confocal Cryo-Fluorescence Microscopy and Cryo-FIB/ SEM Tomography; A Rigort, R Kirmse, V Doering; Carl Zeiss Microscopy; M Schwertner; Linkam Scientific Instruments Ltd; R Wolleschensky; Carl Zeiss Microscopy [560]

1271

Correlative Ultrastructural Analysis of Functionally Modulated Synapses Using Automatic Tape-Collecting Ultramicrotome - SEM Array Tomography; N Kamasawa, Y Sun, T Mikuni, D Guerrero-Given, R Yasuda; Max Planck Florida Institute for Neuroscience [635]

1273

Conjugate Immunofluorescence Ð SEM Array Tomography for Studying Synapses and Other Subcellular Structures in the Brain; KD Micheva; Stanford University School of Medicine [636]

1275

Protocol Development for 3D Reconstructions: Combining In Vivo Imaging, APEX2 and En Bloc Staining of Mouse Visual Cortex; J Buchanan, M Takeno, T Daigle, A Bleckert, D Bumbarger, A Bodor, D Brittain, N Maarico da Costa; Allen Institute for Brain Science et al [637]

1277

Advanced substrate holder and multi-axis manipulation tool for ultramicrotomy; W Spomer,A Hofmann; Institute for Applied Computer Science, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany; I Wacker; Cryo EM, Centre for Advanced Materials, UniversitŠt Heidelberg, Heidelberg, Germany; L Ness, P Brey; RMC Boeckeler Instruments; RR Schršder; Cryo EM, CellNetworks, BioQuant, UniversitŠtsklinikum Heidelberg, Heidelberg, Germany; U Gengenbach; Institute for Applied Computer Science, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany [638]

1493

Dynamics of Thrombus Formation in Mouse Testicular Surface Vein Visualized by Newly Devised ÒVascular MappingÓ Method for Live-CLEM Imaging in vivo; A Sawaguchi, S Nishimura; University of Miyazaki [745]

1495

Cryo-Planing of Frozen Hydrated Samples by Triple Ion Gun Milling (CryoTIGM); IY Chang, D Joester; Northwestern University [746]

1497

Correlative iPALM and Platinum Replica Electron Tomography to Highlight Single Molecules on Clathrin Endocytic Structures in 3D; KA Sochacki, G Shtengel, A Dickey; National Heart Lung and Blood Institute; H Hess; Janelia Farm Research Campus; J Taraska; National Heart Lung and Blood Institute [747]

1499

Correlative Electron and Fluorescence Microscopy of Magnetotactic Bacteria in Liquid: Toward In Vivo Imaging; TJ Woehl, S Kashyap, E Firlar; Ames Laboratory; T Perez-Gonzalez, D Faivre; Max Planck Institute of Colloids and Interfaces; D Trubitsyn, D Bazylinski; University of Nevada at Las Vegas,T Prozorov; Ames Laboratory [748]

1501

Indium-Tin-Oxide (ITO) as Stable and Effective Coating Material for Correlative Confocal and Immuno-Scanning Electron Microscopy Studies; A Falqui, S Rodighiero; King Abdullah University for Science and Technology (KAUST) - Biological and Environmental Sciences and Engineering Division; E Sogne; Fondazione Filarete; B Torre; King Abdullah University for Science and Technology (KAUST) - Physical Sciences and Engineering Division; R Ruffilli; CEMES/CNRS;M Francolini, C Cagnoli; Fondazione Filarete, E Di Fabrizio; King Abdullah University for Science and Technology (KAUST) - Physical Sciences and Engineering Division [749]

1503

Wide Area Observation of Fully Hydrophilic Tissue Achieved by Sliding It on the Dish of the Atmospheric Scanning Electron Microscope (ASEM); C Sato, M Sato, T Ebihara; Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST); H Nishiyama, M Suga; Advanced Technology Division, JEOL Ltd; N Memtily; Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST) [750]

873

Correlative Light and Electron Microscopy Techniques: Challenges and Successes; ES Stempinski, XS Wu; NIH, NHLBI; CA Brantner; The George Washington University [437]

875

Characterizing Microtubule Organization in the Arabidopsis Thaliana Root Apical Meristem via Correlative Microscopy; K Celler, C Ambrose, Y Ruan, B Ross, G Wasteneys; University of British Columbia [438]

877

Cryogenic Sample Preparation Preserves Elemental Composition for Correlative Light and X-ray Fluorescence Microscopy; Q Jin, B Lai; Northwestern University; S chen; Argonne national Lab; T Paunesku; Northwestern University; SC Gleber, L Finney, D vine; argonne national lab,G Woloschak; northwestern university et al [439]

879

Methodology Development at NYULMC Microscopy Core - Correlative Light and Electron Microscopy Applications; F Liang, C Petzold, K Dancel-Manning, Y Deng, M Cammer; New York University Langone Medical Center [440]

881

Investigating Kinetochore Structural Dynamics during Mitosis by Employing Combinations of Several Microscopic Techniques; V Magidson, J He, JG Ault, CB OÕConnell, N Yang, H Sui, BF McEwen,A Khodjakov; Wadsworth Center, New York State Department of Health [441]

883

Cryo-Ultramicrotomy of Complex Molecular Fluids; M Gao; Liquid Crystal Institute, Kent State University [442]

885

Optimized and Improved Immunogold Labeling on Ultrathin Sections of Nervous Tissue Following High Pressure Freezing, Freeze Substitution and Low Temperature Embedding; W-P Li, S Viswanathan, Y Wang; Electron Microscopy Shared Resource, Howard Hughes Medical Institute/ Janelia Research Campus; LL Looger; Looger Laboratory, Howard Hughes Medical Institute/Janelia Research Campus; PK Rivlin; Electron Microscopy Shared Resource, Howard Hughes Medical Institute/Janelia Research Campus [443]

887

Agar Assisted Embedding: Visualizing Plant Pathogen Interactions at The Root Ð Soil Interface;J Kilcrease, J Hammond; United States Department of Agriculture/ORISE, Agricultural Research Service, U S National Arboretum Floral and Nursery Plants Research Unit [444]

889

The Atmospheric Scanning Electron Microscope (ASEM) observes the Cultured Fluorescent Neuron; T Ebihara, M Suga; National Institute of Industrial Science and Technology (AIST); H Nishiyama; JEOL Ltd; C Sato; National Institute of Industrial Science and Technology (AIST) [445]

891

Radiation Damage of Biological Specimen in Environmental Electron Microscopy; J Hermannsdšrfer, N de Jonge; Leibniz Institute for New Materials [446]

B05 3D Structures of Macromolecular Assemblies, Cellular Organelles, and Whole Cells

Heterogeneity in the pre-40S ribosomal subunit reveals two distinct regions of variation at functionally important structural elements; MC Johnson, H Ghalei; Florida State University; K Karbstein; The Scripps Research Institute; ME Stroupe; Florida State University [27]

Determining a Sub-nanometer Resolution Structure of a Helicobacter pylori VacA Toxin Oligomer; TM Pyburn, NJ Foegeding, TL Cover, MD Ohi; Vanderbilt University Medical Center [28]

Sar1 Forms Ordered Arrays That Can Facilitate Vesicle Scission in the COPII Pathway; H Hariri, A Noble, A Omran, S Stagg; Florida State University [29]

Regulation of cytoplasmic dynein by Lis1; K Toropova, S Zou; Harvard University; AJ Roberts,SL Reck-Peterson; Harvard Medical School; AE Leschziner; Department of Molecular and Cellular Biology [30]

541

Modeling Protein Structure in Macromolecular Assemblies at Near Atomic Resolutions; ML Baker, M Chen, PR Baldwin, SJ Ludtke, W Chiu; Baylor College of Medicine [271]

543

IP3R1 Ð Assessing Map Interpretability at Near Atomic Resolution; SJ Ludtke, G Fan, ML Baker, Z Wang; Baylor College of Medicine; MR Baker; University of Texas at Houston Medical School; SC Murray, W Chiu; Baylor College of Medicine, II Serysheva; University of Texas at Houston Medical School [272]

545

Exploiting the Susceptibility of HIV-1 Nucleocapsid Protein to Radiation Damage in Tomo- Bubblegram Imaging; J Fontana; Laboratory of Structural Biology Research, National Institute of Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health; KA Jurado, N Cheng; National Institute of Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health; A Engelman; Dana-Farber Cancer Institute; AC Steven; National Institute of Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health [273]

547

Affinity Cryo-Electron Microscopy Studies of Viral Particles Captured Directly From Cell Culture; C Zhang, F Vago, F Guo, Z Liu, G Yu; Purdue University; P Serwer; University of Texas Health Science Center; W Jiang; Purdue University [274]

1279

Complementary Approaches to Dissecting Mechanisms of Protein-mediated Membrane Fusion;KK Lee, L Gui, J Ebner, NK Garcia, M Guttman; University of Washington [639]

1281

Characterization of Outer Membrane Vesicle Release in Vibrio vulnificus; CM Hampton, RC Guerrero-Ferreira, ER Wright; Emory University [640]

1283

Molecular Characterization of Leading Edge Protrusions in the Absence of Arp2/3 Complex; KL Anderson, C Page; sanford Burnham Medical research Institute; P Suraneni, R Li; Stowers Institute for Medical Research; N Volkmann, D Hanein; SBMRI [641]

1285

_-Synuclein Amyloid Fibrils Formed of Two Protofibrils; AD Dearborn, N Cheng, JB Heymann; Laboratory of Structural Biology Research, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health; JS Wall; Department of Biology, Brookhaven National Laboratory; J Varkey, R Langen; Zilkha Neurogenetic Institute, University of Southern California; AC Steven; Laboratory of Structural Biology Research, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health [642]

1287

Bright-Field STEM Tomography of Blood Platelets in Thick Sections; RD Leapman, GN Calco,JD Hoyne, MM Aronova, BC Kuo, Q He; National Institute of Biomedical Imaging and Bioengineering, NIH; ID Pokrovskaya, LJ MacDonald; University of Arkansas for Medical Sciences et al [643]

893

The 3D-structure analysis of spermatids in the seminiferous epithelia by the serial block-face SEM method; Y Hasebe, T Haruta; JEOL Ltd; N Erdman; JEOL USA inc; M Suga, H Nishioka, T Suzuki; JEOL Ltd [447]

895

Crustacean cuticle: synthesis and remodeling of a dynamic extracellular matrix during molt cycle; J Strus, N Znidarsic; University of Ljubljana; A Blejec, M Tusek Znidaric; National Institute of Biology [448]

897

Structure and Assembly of the Bacillus anthracis Exosporium; CM Rodenburg, SA McPherson, CL Turnbough, T Dokland; University of Alabama at Birmingham [449]

899

Tomographic Analysis of EmaA Adhesin Glycosylation in Aggregatibacter actinomycetemcomitans; A Watson, H Naughton, M Radermacher, KP Mintz, T Ruiz; University of Vermont [450]

901

3D Reconstruction of Mitochondrial Complex I Analyzed After Biogenesis in the Absence of Assembly Factor NB7ML (NDUFAF2); CT Nolan, K Kmita; University of Vermont; V Zickemann; University of Frankfurt/Main; T Ruiz, M Radermacher; University of Vermont [451]

903

Three-Dimensional Visualization of Herpesvirus Envelopment at High Resolution Using STEM Tomography and Serial Sectioning on High Pressure Frozen/Freeze-Substituted Cells; M Schauflinger, C Villinger; Electron Microscopy Core Facility, University of Missouri; J von Einem; Institute of Virology, University Medical Center Ulm; P Walther; Central Facility for Electron Microscopy, Ulm University [452]

905

Ebola virus reconstruction using compressed sensing cryo-electron tomography; A AlAfeef, WP Cockshott; University of Glasgow; IM MacLaren; University of Glasgow [453]

907

Whole-Cell Tomography Using a Conventional Scanning Electron Microscope; T Okumura, M Shoji, A Hisada; Hitachi, Ltd , Central Research Laboratory; Y Ominami; Hitachi High-Technologies Corporation; T Ushiki, M Nakajima; Graduate School of Medical and Dental Sciences, Niigata University; T Ohshima; Hitachi, Ltd , Central Research Laboratory [454]

909

3D Imaging of the Early Embryonic Chicken Heart After Altered Blood Flow with Focused Ion Beam Scanning Electron Microscopy; M Midgett, CS L—pez, S Rugonyi; Biomedical Engineering, Oregon Health Science University [455]

911

Boosting Contrast of Cryo-EM Images Without a Phase Plate; MS Spilman, H Guo, BE Bammes, L Jin, R Bilhorn; Direct Electron, LP [456]

913

The best of both world, how a ÒChristmas treeÓ TEM can please biologist and material scientists; E Hanssen, MP Watts; The University of Melbourne; ER Smith; The Royal Melbourne; P Gosavi, PA Gleeson, JW Moreau; The University of Melbourne [457]

915

Adhesion Pili from Enterotoxigenic Escherichia coli Share Similar Biophysical Properties Despite Their Different Assembly Pathways; N Mortezaei, CR Epler; UmeŒ University; PP Shao; Princeton Univ;M Shirdel, B Singh, BE Uhlin; UmeŒ University; SJ Savarino; Naval Med Research Center, M Andersson; UmeŒ University et al [458]

B06 Deep Tissue Imaging and Light Sheet Microscopy

715

Scanless lattice light sheet microscopy; B-C Chen; Research Center for Applied Science, Academia Sinica [358]

717

Rapid High-resolution Brain Mapping with CLARITY Optimized Light Sheet Microscopy (COLM);R Tomer, K Deisseroth; Howard Hughes Medical Institute [359]

719

Three-color two-photon three-axis digital scanned light-sheet microscopy (3c2p3a-DSLM); L Chen, W Zong; Institute of Molecule Medicine, Peking University; A Wang; State Key Laboratory of Advanced Optical Communication System and Networks, School of Electronics Engineering and Computer Science, Peking University [360]

863

ÔRuptureÕ type thrombopoiesis from bone megakaryocyte is regulated by IL-1alpha: visualization by two photon microscope and software analysis; S Nishimura, A Sawaguchi; Jichi Med Univ, and the Univ of Tokyo [857]

1719

In vivo imaging and software analysis revealed the contribution of endothelial damage to thrombus development processes; S Nishimura, A Sawaguchi; Jichi Med Univ and the Univ of Tokyo [858]

1721

In vivo Three Photon Imaging of Neuronal Activities from Hippocampus in Intact Mouse Brain; T Wang, D Ouzounov, NG Horton, JC Cruz Hern‡ndez, D Feng, N Nishimura; Cornell University [859]

1723

Airy Beams for Light-sheet Microscopy; J Nylk, Z Yang, M Preciado, M Mazilu; University of St Andrews; T Vettenburg; Univ Carlos III de Madrid; C Coll-Llado, DE Ferrier; University of St Andrews, T Cizmar; University of Dundee et al [860]

B07 Microscopy, Microanalysis and Image Cytometry in the Pharmaceutical Sciences

Microscopy, Microbiology and Regulations of Paper and Paperboard Utilized in Pharmaceutical Packaging; JH Woodward; Buckman [31]

Microscopic Techniques for Sterility Assurance Support in the Medical Products Industry; JR Mantei, MA Murphy, L Stojanovic, L Wahlen, M Pasmore, J DiOrio; Baxter Healthcare [32]

Electron Microscopy as an Emerging Analytical Tool for Characterizing Biopharmaceuticals;B Carragher, A Schneemann, JJ Sung, SK Mulligan, JA Speir, K On, J Quispe, CS Potter; NanoImaging Services [33]

391

Simultaneously Measuring Red Blood Cell Flux in vivo for a Large Number of Retinal Capillary Vessels Using Optical Coherence Tomography; RK Wang, Z Zhi; University of Washington [196]

393

Efficient and Documented Preparation of Pharmaceutical Particles for Correlative Microscopy Analyses using mPrepTM Capsule Processing; SL Goodman; Microscopy Innovations LLC [197]

395

Characterization of Glass Delamination by TEM: Results from a New Sample Preparation Technique;EF Schumacher, HM Talesky, KJ Diebold; McCrone Associates, Inc [198]

397

Prospects for High Resolution Analytical Electron Microscopy of Organic Crystalline Particles; J Cattle, M SÕari; University of Leeds; N Wilkinson; Gatan UK; N Hondow, A Brown, R Brydson; University of Leeds [199]

549

Using Microscopy to Qualitatively and Quantitatively Assess Crystalline Content in Amorphous Active Pharmaceutical Ingredients and Drug Product; A Vogt, J Neilly; AbbVie Inc [275]

551

Advantages of Using Low Voltage for Elemental Mapping by Energy Dispersive X-Ray Spectroscopy in Pharmaceutical Systems; JP Neilly, JR Roth; AbbVie, Inc [276]

553

Streamlining Identification of Compounds in Pharmaceutical Drug Products; T Nylese, J Rafaelsen, M Bolorizadeh; EDAX, A Division of Ametek, Materials Analysis Division; D Edwards; JEOL [277]

191

Analysis of Glass Vial Interior Surfaces in Parenteral Drug Stability Studies; RA Carlton; GlaxoSmithKline [96]

193

ESEM Study of Magnetic Bubbles Ð Surface Morphological and Fluidic Aspects; Z Barkay, Y Gao; Wolfson Applied Materials Research Center, Tel-Aviv University; C Xu; Division of Bioengineering, Nanyang Technological University; C-D Ohl; Division of Physics and Applied Physics, Nanyang Technological University [97]

195

Measurement of radiation-induced autophagic flux in human brain cancer cells; LS Yasui, M Johnson, H Savage, A Baker, E Baker, C Burns; Northern Illinois University [98]

B08 Dynamic Fluorescence Microscopy

Imaging the dynamic release and capture of vesicle membrane proteins in mammalian cells;JW Taraska, KA Sochacki, BT Larson; NHLBI, NIH [34]

Focal Adhesion Kinase Anchoring Kinetics and Regulatory Interactions Quantified by Total Internal Reflection Fluorescence Microscopy; T Zak, AM Samarel, SL Robia; Loyola University Chicago [35]

The Biogenesis of Endophilin B1 Containing Vesicles; Y Chen, J li, JD Mueller; University of Minnesota; B Barylko, JP Albanesi; UT Southwestern Medical Center [36]

197

Sparse Feature Selection Identifies H2A.Z as a Novel, Pattern-Specific Biomarker for Asymmetrically Self-Renewing Distributed Stem Cells; YH Huh, M Noh; Korea Basic Science Institute; FR Burden; CSIRO Manufacturing Flagship; JC Chen; The Senator Paul D Wellstone Muscular Dystrophy Cooperative Research Center; DA Winkler; CSIRO Manufacturing Flagship; JL Sherley; Asymmetrex; H-S Kweon; Korea Basic Science Institute [99]

199

Novel Regulated Secretion Mechanism For a Nerve-Secreted Cell Signaling Molecule in C. elegans; RD Schultz, K Beifuss, S Bageshwar; Texas AM University Health Science Center; EA Ellis; Texas AM University; TL Gumienny; Texas WomanÕs University [100]

201

Mechanics of Interdigitating Morphogenesis in Pavement Cells; A Jafari Bidhendi, B Altartouri, A Geitmann; Institut de Recherche en Biologie VŽgŽtale, University of Montreal [101]

B09 Utilizing Microscopy for Research and Diagnosis of Diseases in Humans, Plants, and Animals

Ebola Virus Hemorrhagic Fever; CS Goldsmith; Centers for Disease Control and Prevention [37]

Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by TEM; B Zechmann, G Zellnig; Baylor University [38]

Immunoelectron Microscopy Reveals Varied Surface Expression of Potential Vaccine Targets of Chlamydia trachomatis; R-C Hsia, JM Sotiris, PM Bavoil; University of Maryland Baltimore [39]

EM Detection of Viruses in Organ Transplant Patients; SE Miller, DN Howell; Duke University [40]

399

Quick Observation of Tissues in Solution by Atmospheric Scanning Electron Microscope (ASEM); N Memtily, M Sato; Graduate School of Comprehensive Human Sciences, University of Tsukuba; T Okada; Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST); T Ebihara; Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST); K Mio; Graduate School of ComprehensiveHuman Sciences, University of Tsukuba; M Suga, N Hiditoshi; Advanced Technology Division, JEOL Ltd., C Sato; Graduate School of Comprehensive Human Sciences, University of Tsukuba [200]

401

Elemental Analysis of RatÕs Femoral Neck with Experimental Diabetes by means of Scanning Electron Microscopy; XS Ram’rez-G—mez, E Rodr’guez-Miranda; Universidad de Guanajuato Campus Celaya; R Vargas-Bernal, G Herrera-PŽrez; Instituto Tecnol—gico Superior de Irapuato [201]

403

MacCallumÕs Triangle Ð Is It Rheumatic? Is It Traumatic? Or Is It Both?; S Siew; Michigan State University [202]

405

Nanoscale 3D Refractive Indices Mapping on Native Cheek Cells by Axial Scanning Transmission Electron Tomography; Y Li, J Wu, R Bleher, V Dravid, V Backman; Northwestern University [203]

407

Is Electron Microscopy Relevant Anymore in Diagnosis of Disease?; WT Ginning; University of Toledo [204]

555

Mitochondrial fission arrest phenotype in brain tissue of patients and animal models of familial AlzheimerÕs Disease revealed with 3D EM; E Trushina, TA Christensen, L Zhang, S Trushin,JL Salisbury; Mayo Clinic [278]

557

A Tunable Approach to Visualize BRCA1 Assemblies in Hereditary Breast Cancer; CE Winton, BL Gilmore, AC Demmert, Z Sheng, DF Kelly; Virginia Tech Carilion School of Medicine and Research Institute [279]

559

mApoE-Functionalized Nanoliposomes Delivering Doxorubicin and Ultrasmall Superparamagnetic Iron Oxide to Glioblastoma Cells Characterized by TEM and Confocal Microscopy; S Rodighiero, M Gregori; Fondazione Filarete; M Francolini, E Vezzoli, M Tamborini; University of Milan;M Masserini; University of Milano-Bicocca; M Matteoli; Humanitas Clinical and Research Center, L Passoni; University of Milan [280]

561

Type VII Collagen: From Discovery of the Anchoring Fibril Protein to Clinical Trials Ameliorating the Human Blistering Disease Epidermolysis Bullosa; DR Keene, SF Tufa; Shriners Hospital for Children [281]

203

Loss of STARD7 Expression Compromises Epithelial Barrier Integrity in Airway of the Shh+/-Cre/ STARD7-/- Mice; C-L Na, L Yang, TE Weaver; Cincinnati ChildrenÕs Hospital Med Ctr [102]

205

FIB/SEM Tomography of Wound Biofilm; B Deng, KG Barki, S Ghatak, S Roy, DM McComb, CK Sen; The Ohio State University [103]

207

Visualization of Plaque/Vessel Interfaces using FIB-SEM; G Wetzel, SM Lessner; Clemson University [104]

209

Enhanced Large-Area TEM Analysis on Mitochondrial Alteration in Brain Neuronal Cell of ParkinsonÕs Disease Model Mouse Using Montage Function Installed in JEM 1400-Plus System;H-J Kim, AR Je, MJ Kim, J-K Hyun; Korea Basic Science Institute; H-G Kim; Dankook University; YH Huh, H-S Kweon; Korea Basic Science Institute [105]

211

Renal Biopsy Diagnosis of IGA Nephropathy in Chin Refugees living in the United States; N Rush, MP Goheen; Indiana University School of Medicine/Pathology; CL Phillips; Indiana University School of Medicine/Pathology [106]

213

Visualizing the Distribution and Stoichiometry of Growth Factor Receptors in Intact Cells in Liquid Phase with Correlative Fluorescence and Scanning Transmission Electron Microscopy; DB Peckys, J Hermannsdšrfer, V Tinnemann; Leibniz Institute for New Materials; U Korf; German Cancer Research Center; N de Jonge; Leibniz Institute for New Materials [107]

215

Centrin Phosphorylation and Localization Dynamics During The Centriole Cycle; TA Christensen, JL Salisbury; Mayo Clinic [108]

217

Immunofluorescence Microscopy As a Critical Confirmation Technique in Cell Cycle-Related Proteomics Studies; SL Faitar; DÕYouville College [109]

219

Using Optical Tweezers to Quantify the Interaction Force of Dengue Virus with Host Cellular Receptors; B Tsai; Institute of Biophotonics, National Yang-Ming University [110]

221

Application of FRET microscopy for visualizing the uncoating process of a single Dengue virus in living cells; L-W Chu, Y-H Lin; National Yang-Ming University; C-H Lee; Research Center for Applied Sciences, Academia Sinica; Y-H Ping; National Yang-Ming University [111]

223

The Observation of Saccharomyces cerevisiae Ultrastructure Changes under Proline Limitation;H Chen, X Liang; Microscopy Core Facility, Center of Biotechnology, University of Nebraska Lincoln; D Becker; University of Nebraska Lincoln [112]

225

The Wisconsin Electron Microscopy Diagnostic Proficiency Program; CA Radi, SE Miller; Wisconsin Veterinary Diagnostic Laboratory; C Goldsmith; Centers for Disease Control and Prevention;K Toohey-Kurth; Wisconsin Veterinary Diagnostic Laboratory [113]

227

Wheat Leaf Rust Fungus: RIMAPS Analysis to Detect Germ-tube Orientation Pattern; LM Setten, N Lendoiro, EA Favret; Instituto Nacional de Tecnolog’a Agropecuaria (INTA) [114]

229

Ice Contamination Issues in the Visualization of the Ultrastructure of the Nuclear Envelope by Freeze-Fracture Technique; N Vaskovicova, V Krzyzanek, A Patak; Institute of Scientific Instruments of the ASCR [115]

231

Imaging of Polyethylene Glycol Layers on Nanoparticles; SR Anderson, MC Smith; Electron Microscopy Laboratory, Cancer Research Technology Program, Leidos Biomedical Research, Inc, Frederick National Laboratory for Cancer Research; JD Clogston, AK Patri, SE McNeil; Nanotechnology Characterization Laboratory, Cancer Research Technology Program, Leidos Biomedical Research, Inc, Frederick National Laboratory for Cancer Research; U Baxa; ElectronMicroscopy Laboratory, Cancer Research Technology Program, Leidos Biomedical Research, Inc, Frederick National Laboratory for Cancer Research [116]

233

Treatment of Influenza-Induced Acute Lung Injury with Iron Oxide Nanoparticles using an Ischemic- Reperfusion Model; K Chew, A Waldron, C Queenan, R Pergolizzi; Bergen County Academies;T Hoffmann; Englewood Hospital and Medical Center [117]

235

Structure of Microtubule-Based Microtentacles; AN Killilea, R Csencsits; Lawrence Berkeley National Laboratory; S Kenny, K Xu; University of California Berkeley; KH Downing; Lawrence Berkeley National Laboratory [118]

237

Bartonella spp. biofilm identified in patients; ME Ericson, M Weber-Sanders; University of Minnesota; M Neves da SIlva; State University of Campinas (UNICAMP) Medical Sciences School; VB Pelegati,CL Cesar; National Institute of Science and Technology on Photonics Applied to Cell Biology (INFABIC); G Vieira-Damiani, PE Velho; State University of Campinas (UNICAMP) Medical Sciences School [119]

B10 Multiscale Biological Imaging: From Micro to Macro - Animal to Clinical Models

In-situ Topographical and Elemental Characterisation of Biological Soft Matter; CL Collins, C McCarthy; Oxford Instruments NanoAnalysis [41]

Biomineralization at Interfaces Revealed with 4D Electron and Atom Probe Tomographies; X Wang, B Langelier; McMaster University; A Palmquist; University of Gothenburg; K Grandfield; McMaster University [42]

Magnetotactic Bacteria and Honey Bees: Model Systems for Characterising an Iron Oxide Mediated Magnetoreceptor; J Shaw, A Boyd; Centre for Microscopy, Characterisation and Analysis, The University of Western Australia; M House; Biomagnetics Group, The University of Western Australia; M Saunders; Centre for Microscopy, Characterisation and Analysis, The University of Western Australia; F Mathes; School of Earth and Environment, The University of Western Australia; B Baer; Centre for Integrative Bee Research (CIBER), The University of Western Australia [43]

Development of a Bioinspired Stroma Model to Study the Role of Collagen Topology in Pancreatic Ductal Adenocarcinoma; C Drifka, A Loeffler, K Eliceiri, J Kao; University of Wisconsin-Madison [44]

From Image Tiles to Web-Based Interactive Measurements in One Stop; A Vandecreme, T Blattner, M Majurski, P Bajcsy, K Scott, JHJ Scott; NIST [45]

409

Imaging the alphavirus exit pathway; MG Martinez, E-L Snapp; Department of Cell Biology, Albert Einstein College of Medicine; GS Perumal, FP Macaluso; Analytical Imaging Facility, Albert Einstein College of Medicine; M Kielian; Department of Cell Biology, Albert Einstein College of Medicine [205]

411

Localization and Number of Au Nanoparticles in Optically Indexed Cells Using FIB Tomography;AW Sanders, KM Jeerage, AE Curtin, AN Chiaramonti; NIST [206]

413

Swept Confocally-Aligned Planar Excitation (SCAPE) Microscopy for High Speed Volumetric Imaging in Behaving Animals; EM Hillman; Columbia University [207]

415

Correlative large volume imaging across scales; RR Schroeder, H Blank; UniversitŠtsklinikum Heidelberg, UniversitŠt Heidelberg; A Schertel, M Thaler, A Orchowski; Carl Zeiss Microscopy GmbH; I Wacker; UniversitŠt Heidelberg [208]

917

X-ray Tomographic Microscopy of Drosophila Brain Network and Skeletonized Model Building in the Three-Dimensional Image; R Mizutani, R Saiga; Tokai University; K Uesugi, A Takeuchi, Y Terada, Y Suzuki; Japan Synchrotron Radiation Research Institute (JASRI/SPring-8) [459]

919

Three-Dimensional Neuronal Structure of Human Cerebral Cortex Determined by Synchrotron- Radiation Microtomography; R Saiga, S Takekoshi; Tokai University; C Inomoto, N Nakamura,A Tsuboi, M Osawa; Tokai University School of Medicine; M Arai, K Oshima; Tokyo Metropolitan Institute of Medical Science et al [460]

921

A Reverse Engineering Approach for Imaging Spinal Cord Architecture Ð Large Area High-Resolution SEM Imaging; CA Brantner, M Rasche; The George Washington University, GW Center for Nanofabrication and Imaging; KE Burcham, J Klingfus, JE Sanabia; International Applications Center, Raith America, Inc; CE Korman, A Popratiloff; The George Washington University, GW Center for Nanofabrication and Imaging [461]

923

Simplified Biological Tissue Analysis with Combination of Atmospheric Scanning Electron Microscope and Light Microscope; A Hisada, Y Ominami; Hitachi Central Research Laboratory [462]

925

3D X-ray Microscopy: A New High Resolution Tomographic Technology for Biological Specimens;L Lavery, A Merkle; Carl Zeiss X-ray Microscopy [463]

B11 Specimen Preparation for Biological Sciences

721

Easier and Safer Biological Staining: High Contrast UranyLess Staining of TEM Grids using mPrep/g Capsules; N Benmeradi, B Payre; Delta Microscopies; SL Goodman; Microscopy Innovations LLC [361]

723

HSV-1 Scaffolding Protein Bubbles Readily in the Absence or Presence of DNA, Allowing its Localization in Immature and Mature Nucleocapsids; DC Winkler, WW Newcomb, A Aksyuk, W Wu, N Cheng, AC Steven; National Institutes of Health [362]

725

Improving the Reliability, Ease, and Efficiency of Section Staining in a Diagnostic Laboratory with the mPrep/g System for TEM Grid Processing; CA Radi; Wisconsin Veterinary Diagnostic Laboratory [363]

727

Capabilities of a New Sample Preparation Method for Probing Polymer Penetration and Localization into Wood Without Embedding; B Dorvel, P Boopalachandran; Dow Chemical Company- Analytical Sciences; A Bowling; Dow Agrosciences- Advanced Technology Development; X Chen, S King; Dow Chemical Company- Industrials RD [364]

927

Cryo Transmission Electron Microscopy of Three Research-Grade Liposomal Formulations of Doxorubicin; W Monroe, A Paredes; U S Food and Drug Administration, National Center for Toxicological Research; P Sisco; U S Food and Drug Administration, Office of Regulatory Affairs; K Ahlschwede; U S Food and Drug Administration, National Center for Toxicological Research; S Linder; U S Food and Drug Administration, Office of Regulatory Affairs [464]

929

Using a White Light Confocal Profiler for Ancient Diet Reconstruction; ARJ Remy, C Schmidt; University of Indianapolis; R DÕAnastasio; Museo Universitario Universitˆ [465]

931

Testate Amoebae Diversity, from the Atlantic Forest Aquatic and Edaphic Environments, Collected Within the Rio de Janeiro State, Brazil; ID da Silva-Neto, B Leite; Dep de Zoologia, Inst de Biologia [466]

933

Inactivation of Bacterial Endospores in an Artificial Tissue for Electron Microscopy Analysis;RM Hannah, BM Leroux, RK Pope; NBACC [467]

935

Aspergillus fumigatus Biofilms: a Comparison of Processing Techniques for Scanning Electron Microscopy of Fungal Mycelium and Extracellular Matrix; L-M Joubert, JA Ferreira, DA Stevens, L Cegelski; Stanford University [468]

937

Sure, We Can Image That! An Attempt to Image the Edge of a Growing Bacterial Colony; GD Martens, K Hodgson; University of British Columbia [469]